Interspecific hybridization creates new genetic variants. Embryo formation and subsequently fertile seed development are the indicators of successful interspecific hybridization. Often interspecific hybridization is limited due to variations in genome and ploidy levels. The technique of embryo rescue is commonly used in interspecific hybridization to facilitate the survival of embryos from abortion. The effectiveness of an embryo rescue program in the
Citations
Morphological variation was investigated among 31 maize landrace accessions collected from the fields of various farmers in South Sudan by examining 7 quantitative agronomic characteristics. A significant positive correlation was found between days to tasseling (DT) and days to silking (DS), between plant height (PH) and ear height (EH) and stem width (SW), and between EH and SW and leaf length (LL). First and second principal components accounted for 69% of the total variance (40.9% and 28.6%, respectively). PH, EH, SW, LL, and leaf width (LW) were clearly characterized in a positive direction on the first axis. DT and DS were clearly characterized in a positive direction on the second axis. A scatter plot based on phenotypic data revealed the existence of 3 groups based on the most discriminating characteristics: Group I included 5 maize landrace accessions, Group II comprised 14 maize landrace accessions, and Group III included 10 maize landrace accessions. In principal component analysis, generally the investigated genotypes were not clearly grouped into their geographical origins owing to a weak geographic relationship among the accessions. In conclusion, even though the morphological characterization studies were conducted in the South Korean climate, this study revealed significant phenotypic variation among the explored maize landrace accessions collected from South Sudan. Therefore, this information about phenotypic divergence may be very useful for future breeding research programs as well as for genetic improvement of South Sudan maize accessions.
Citations
Submergence damage to rice was reported as one of the major problems in rainfed lowland areas where the water remains. This study assessed the submergence tolerance of core collection during the seedling stage of the rice using dry seeds. Also, genome-wide association study (GWAS) combined with principal component analysis (PCA) and kinship matrix analysis was performed to identify quantitative trait loci (QTL) for submergence tolerance. Through this GWAS analysis, nine lead SNPs were confirmed to be associated with submergence tolerance, and a linkage disequilibrium (LD) decay analysis identified the 230 kb exploratory range for the detection of QTLs and candidate genes. Nine QTL were detected, on chromosomes 3 (
Citations
Citations
The conventional soybean breeding program by single seed descent method required around 8 to 9 years to develop a cultivar. Through the advancement of breeding techniques, such as marker-assisted selection, required resources could be significantly saved, but the generation advancement step still slows down the entire soybean breeding program time course. In this study, 28 soybean varieties were tested to find the optimal speed breeding conditions for soybeans that could rapidly advance one generation with 2 light sources, 3 light conditions, and 2 planting densities. Plants were kept under short-day conditions (9 hours light/15 hours dark). We optimized the growth conditions for shortening the period of soybean generation progression based on speed breeding. The optimizing conditions are as follows. (1) Irradiation using LED light source for 9 hours, (2) 506 mmol/(m2∙s) of PPFD at 30 cm from the ground, (3) Planting density of 5 cm × 5 cm, (4) temperature of 25℃ ± 2℃ and (5) humidity of 50% ± 10%. If this condition is used, soybeans can be advanced by one generation within an average of 73 days. It is possible to advance five generations a year using only indoor speed-breeding system. Furthermore, if it includes the development of lines in the field, four generation per year, which is advance three generations using indoor speed-breeding system and one generation in the field, is allowed to increase soybean breeding speed with minimum input.
Citations
Amylose content is a key factor affecting the eating and cooking qualities of rice. In our previous study,