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Volume 4(3); August 2016

Review Article

Current Applicable DNA Markers for Marker Assisted Breeding in Abiotic and Biotic Stress Tolerance in Rice (Oryza sativa L.)
Franz Marielle Nogoy, Jae-Young Song, Sothea Ouk, Shadi Rahimi, Soon Wook Kwon, Kwon-Kyoo Kang, Yong-Gu Cho
Plant Breed. Biotech. 2016;4(3):271-284.   Published online August 31, 2016
DOI: https://doi.org/10.9787/PBB.2016.4.3.271

Abiotic and biotic stresses adversely affect rice (Oryza sativa L.) growth and yield. Conventional breeding is a very effective method to develop tolerant rice variety; however, it takes a decade long to establish a new rice variety. DNA-based markers have a huge potential to improve the efficiency and precision of conventional plant breeding via marker-assisted selection (MAS). The large number of quantitative trait loci (QTLs) mapping studies for rice has provided an abundance of DNA marker-trait associations. The limitations of conventional breeding such as linkage drag and lengthy time consumption can be overcome by utilizing DNA markers in plant breeding. The major applications of DNA markers such as MAS, QTL mapping and gene pyramiding have been surveyed. In this review, we presented the latest markers available for some of the most important abiotic and biotic stresses in rice breeding programs. Achieving a significant impact on crop improvement by marker assisted breeding (MAB) represents the great challenge for agricultural scientists in the next few decades.

Citations

Citations to this article as recorded by  
  • Efficiency of doubled haploid technology in mining of multiple BB resistance genes from indica rice hybrid
    Sudhansu Sekhar Bhuyan, Byomkesh Dash, Prachitara Rout, Nupur Naik, Manjusha Chandravani, Nibedita Swain, Ram Lakhan Verma, Jawahar Lal Katara, Arup Kumar Mukherjee, C. Parameswaran, BN. Devanna, Snigdha Samir Pattnaik, Sanghamitra Samantaray
    Cereal Research Communications.2025; 53(3): 1319.     CrossRef
  • The Development of Multi-Resistant Rice Restorer Lines and Hybrid Varieties by Pyramiding Resistance Genes against Blast and Brown Planthopper
    Zhao Deng, Peng Qin, Kaiyu Liu, Nan Jiang, Tianze Yan, Xuanwen Zhang, Chenjian Fu, Guangcun He, Kai Wang, Yuanzhu Yang
    Agronomy.2024; 14(5): 878.     CrossRef
  • Emerging Trends in Wheat (Triticum spp.) Breeding: Implications for the Future
    Mujahid Alam, P. Stephen Baenziger, Katherine Frels
    Frontiers in Bioscience-Elite.2024;[Epub]     CrossRef
  • Genomics and transcriptomics to protect rice (Oryza sativa. L.) from abiotic stressors: -pathways to achieving zero hunger
    Mushtaq Ahmad
    Frontiers in Plant Science.2022;[Epub]     CrossRef
  • Advances from Conventional to Modern Plant Breeding Methodologies
    Sashi Lamichhane, Sapana Thapa
    Plant Breeding and Biotechnology.2022; 10(1): 1.     CrossRef
  • Review on harnessing biotechnological tools for the development of stable bacterial wilt resistant solanaceous vegetable crops
    Shweta Sharma, Viveka Katoch, Devinder Kumar Banyal
    Scientia Horticulturae.2021; 285: 110158.     CrossRef
  • Genotyping for Blast (Pyricularia oryzae) Resistance Genes in F2 Population of Supa Aromatic Rice (Oryza sativa L.)
    L. Kanyange, J. Kamau, O. Ombori, A. Ndayiragije, M. Muthini
    International Journal of Genomics.2019; 2019: 1.     CrossRef
  • Breeding Hybrid Rice with Genes Resistant to Diseases and Insects Using Marker-Assisted Selection and Evaluation of Biological Assay
    Me-Sun Kim, Sothea Ouk, Kuk-Hyun Jung, Yoohan Song, Le Van Trang, Ju-Young Yang, Yong-Gu Cho
    Plant Breeding and Biotechnology.2019; 7(3): 272.     CrossRef
  • Insight into MAS: A Molecular Tool for Development of Stress Resistant and Quality of Rice through Gene Stacking
    Gitishree Das, Jayanta Kumar Patra, Kwang-Hyun Baek
    Frontiers in Plant Science.2017;[Epub]     CrossRef
  • Application and utilization of marker assisted selection for biotic stress resistance in hybrid rice (Oryza sativaL.)
    Jae-Young Song, Sothea Ouk, Franz Marielle Nogoy, Marjohn C. Niño, Soon Wook Kwon, Woongoo Ha, Kwon-Kyoo Kang, Yong-Gu Cho
    Journal of Plant Biotechnology.2016; 43(3): 317.     CrossRef
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Research Articles

Next-Generation Sequencing Based Transposon Display to Detect High-Throughput Insertion Polymorphism Markers in Brassica
Sampath Perumal, Nomar Espinosa Waminal, Jonghoon Lee, Nur Kholilatul Izzah, Mina Jin, Beom-Soon Choi, Tae-Jin Yang
Plant Breed. Biotech. 2016;4(3):285-296.   Published online August 31, 2016
DOI: https://doi.org/10.9787/PBB.2016.4.3.285

Miniature transposable elements (mTEs) such as miniature inverted-repeat transposable element (MITE), terminal repeat retrotransposon in miniature, and short interspersed element are exquisite sources for marker development. mTEs are short, non-autonomous and stably inherited. The high-copy members are widely distributed into the gene rich euchromatic regions. Here, we conducted a modified transposon display (TD) for a high-copy MITE family, BraSto-2 (Bs2). The Bs2-specific primers derived from conserved sequences of Bs2 members as well as MseI adapter primers were used for polymerase chain reaction (PCR) in two Brassica rapa accessions, ‘Chiifu’ and ‘Kenshin’. The pooled PCR products were sequenced by Illumina sequencing platform instead of high-resolution gel electrophoresis. Subsequent in silico-based insertion polymorphism (IP) analysis (next-generation sequencing [NGS]-based Bs2 transposon display) was conducted, which generated more than 99 putative polymorphic insertion sites between ‘Chiifu’ and ‘Kenshin’. Among 90 successful PCR amplification, 34 showed Bs2 IP (IP-Bs2) between ‘Chiifu’ and ‘Kenshin’ accessions, 27 and seven ‘Chiifu’- and ‘Kenshin’-unique insertions, respectively. When the 90 IP-Bs2 primer sets were applied to 10 Brassica accessions, including four additional B. rapa and B. oleracea accessions, 69 (76%) showed insertion olymorphism among accessions. The IP-Bs2 were evenly distributed through all the chromosomes and provide rich polymorphism among various B. rapa and B. oleracea accessions demonstrating the usefulness of these markers for various genetic diversity and molecular breeding studies in Brassica. In addition, NGS-based TD will be applicable to various high copy transposable elements family for high throughput and rapid polymorphic marker development which will be helpful for efficient plant genomics and breeding purposes.

Citations

Citations to this article as recorded by  
  • Nuclear and chloroplast genome diversity revealed by low-coverage whole-genome shotgun sequence in 44 Brassica oleracea breeding lines
    Sampath Perumal, Nomar Espinosa Waminal, Jonghoon Lee, Hyun-Jin Koo, Boem-soon Choi, Jee Young Park, Kyounggu Ahn, Tae-Jin Yang
    Horticultural Plant Journal.2021; 7(6): 539.     CrossRef
  • Miniature inverted-repeat transposable elements (MITEs), derived insertional polymorphism as a tool of marker systems for molecular plant breeding
    Venkatesh, B. Nandini
    Molecular Biology Reports.2020; 47(4): 3155.     CrossRef
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Characterization of Selected Rice Varieties Adapted in Africa
Abebe Megersa, Jeonghwan Seo, Joong Hyoun Chin, Backki Kim, Hee-Jong Koh
Plant Breed. Biotech. 2016;4(3):297-305.   Published online August 31, 2016
DOI: https://doi.org/10.9787/PBB.2016.4.3.297

Rice varieties developed through the inter-specific crosses between Asian (Oryza sativa L.) and African cultivated rice (O. glaberrima Steud.) have contributed to yield enhancement of African rice. However, the genetic diversity and genetic structure of the African varieties have rarely been reported. In this study, we characterized 40 rice varieties including rice released in Africa based on eleven yield related agronomic traits and 96 single nucleotide polymorphism (SNP) markers. They were grouped into three categories based on the cluster analysis using agronomic traits. Meanwhile, they were grouped into two distinct clusters, indica- and japonica-type, based on the genetic distance using 96 SNP markers. CG14, which belongs to O. glaberrima, was found to be an admixture type between indica and japonica. The number of secondary branches (SBs) was the only agronomic trait which was significantly different between two groups. The results of this study provide basic agronomic and genomic information of cultivated and tested rice varieties for Africa, that would be helpful for further rice varietal improvement in Africa.

Citations

Citations to this article as recorded by  
  • Analysis of Agricultural Traits of O. sativa and O. glaberrima under Korean Climatic Conditions
    Jae-Ryoung Park, Hyun-Su Park, Jeonghwan Seo, Chang-Min Lee, Songhee Park, Mina Jin, Keon Mi Lee, Keunpyo Lee, Sukyeung Lee, Ebrima Jallow, O-Young Jeong
    Korean Journal of Breeding Science.2024; 56(2): 97.     CrossRef
  • QTL Analysis of Rice Grain Size Using Segregating Populations Derived from the Large Grain Line
    Ja-Hong Lee, Jeonghwan Seo, San Mar Lar, Seong-Gyu Jang, Hongjia Zhang, Ah-Rim Lee, Fang-Yuan Cao, Na-Eun Kim, Joohyun Lee, Soon-Wook Kwon
    Agriculture.2021; 11(6): 565.     CrossRef
  • Development and application of indica–japonica SNP assays using the Fluidigm platform for rice genetic analysis and molecular breeding
    Jeonghwan Seo, Gileung Lee, Zhuo Jin, Backki Kim, Joong Hyoun Chin, Hee-Jong Koh
    Molecular Breeding.2020;[Epub]     CrossRef
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Genetic Diversity and Population Structure of Asian Tomato Accessions Based on Simple-Sequence Repeats
Sebastin Raveendar, Jong-Wook Chung, Gi-An Lee, Jung-Ro Lee, Kyung-Jun Lee, Myoung-Jae Shin, Yang-Hee Cho, Kyung-Ho Ma
Plant Breed. Biotech. 2016;4(3):306-314.   Published online August 31, 2016
DOI: https://doi.org/10.9787/PBB.2016.4.3.306

Tomato (Solanum lycopersicum L.) is one of the most economically important plants in the family Solanaceae. Understanding its genetic diversity of accessions is vital for additional collection of tomato germplasms. The
objective
of this study was to determine the genetic diversity and population structure of 355 tomato accessions from Asia using 18 simple-sequence repeats (SSRs). A total of 176 alleles were detected at an average of ten alleles per SSR locus. The average major allele frequency and polymorphic information content were 0.69 and 0.39, respectively. Model-based structure analysis revealed two subpopulations (88%), including admixtures (11%) in the 355 Asian tomato accessions, consistent with clustering results based on genetic distance. The overall FST value was 0.135, indicating a moderate differentiation between the inferred subpopulations. Analysis of molecular variance showed that the genetic variance among geographical groups was less than 6%, in contrast to 86% of genetic variance among individuals. The results from this study will provide important information for future germplasm conservation and improvement programs for tomato.

Citations

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  • Next generation sequencing technologies to explore the diversity of germplasm resources: Achievements and trends in tomato
    Pasquale Tripodi
    Computational and Structural Biotechnology Journal.2022; 20: 6250.     CrossRef
  • Genetic diversity, population structure and validation of SSR markers linked to Sw-5 and I-2 genes in tomato germplasm
    Saidaiah Pidigam, Vishnukiran Thuraga, Suchandranath Babu Munnam, Geetha Amarapalli, Gopal Kuraba, Someswara Rao Pandravada, Srinivas Nimmarajula, Hari Kishan Sudini
    Physiology and Molecular Biology of Plants.2021; 27(8): 1695.     CrossRef
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Developing PCR-Based SNP Markers for Distinguishing Korean Waxy Corn F1 Hybrids
Sang Gon Kim, Jin-Seok Lee, Seonghyu Shin, Hwan Hee Bae, Jung-Tae Kim, Beom-Young Son, Seong-Bum Baek
Plant Breed. Biotech. 2016;4(3):315-323.   Published online August 31, 2016
DOI: https://doi.org/10.9787/PBB.2016.4.3.315

Single nucleotide polymorphisms (SNPs) are abundantly and evenly distributed throughout the genomes of most plant species. These markers have become popular for use in genetic research in many crops. SNP markers can be used to screen maize cultivars rapidly during the early growth stages. In this study, to develop additional SNP markers for maize, we chose 20 SNP sites per chromosome from the maizeGDB website (www.maizegdb.org) and designed primers with two base pair mismatches using Primer Designer 4 based on putative SNP sites of the B73 genome sequence. The polymerase chain reaction (PCR) products ranged from 200 to 500 bp in size, whereas no PCR product was detected when the SNP site was present in Korean waxy corn. Using nine Korean commercial F1 hybrids of waxy corn, including Chalok 1, Chalok 4, Ilmichal, Eolrukchal 1, Heukjinjuchal, Hayanchal 95, Mibaekchal, Mibaek 2, and Miheukchal, we selected 16 primer sets showing clear bands or no bands. Based on cluster analysis, we confirmed that the nine Korean waxy corn hybrids could clearly be distinguished. The SNP marker sets are easy to utilize through simple PCR and agarose gel electrophoresis. These results suggest that analysis using the SNP marker set designed in this study would be faster, cheaper, and more reproducible than that using other genotyping tools, such as cleaved amplified polymorphic sequence markers, which require the use of restriction enzymes.

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Expression of Genes Related with Defense Responses against Pathogen Infections in Vitis flexuosa
Md Zaherul Islam, Soon Young Ahn, Seon Ae Kim, Yong-Bum Kwack, Hae Keun Yun
Plant Breed. Biotech. 2016;4(3):324-335.   Published online August 31, 2016
DOI: https://doi.org/10.9787/PBB.2016.4.3.324

Induced defense responses are regulated through a network of signal transduction pathways. Major signal transduction pathways in plants include salicylic acid (SA)- and jasmonic acid (JA)-mediated signaling pathways. In this study, we attempted to identify potential resistance mechanisms/pathways in Vitis flexuosa and the interaction between resistance and resistance related genes of V. flexuosa and several pathogens. To accomplish this, we investigated transcriptional expression of genes in the SA- and JA-mediated pathway and in the flavonoid biosynthesis pathway in V. flexuosa that had been infected by Elsinoë ampelina, Botrytis cinerea, Colletotrichum acutatum, Erysiphe necator, and Rhizobium vitis using real-time polymerase chain reaction (PCR) analysis. Quantitative real-time PCR revealed that R and R-related genes in V. flexuosa induced defense responses through expression of genes in SA-pathway rather than in the JA-pathway, and R-related genes were more closely correlated with lignin and phytoalexin biosynthesis than anthocyanin biosynthesis against different pathogen infections in V. flexuosa. Specifically, genes such as VfRLK586, VfRLK2422, VfRLK5099, VfRLK29610, VfRLK55257, VfRPS5-like4135, VfRPS5-like4832, VfRPS5-like20585, VfRPS5-like55532, VfEDL2, VfEDL3, VfCXE5585, VfCXE12827, and VfCXE13132 showed close correlation with induction of genes in the SA-mediated pathway, lignin, phytoalexin, and anthocyanin biosynthesis. Genes including VfRLK2422, VfRLK5099, VfRLK29610, VfRPS5-like55532, VfEDL3, and VfCXE12827 were also correlated with the JA-mediated signaling pathway in induction of defense responses.

Citations

Citations to this article as recorded by  
  • Mining of differentially expressed genes from Korean wild grapes responding to grapevine leaf rust pathogen (Phakopsora euvitis) infection
    Zar Le Myint, Srinivasan Ramalingam, Soon Young Ahn, Hae Keun Yun
    Horticulture, Environment, and Biotechnology.2024; 65(4): 607.     CrossRef
  • Induction of defense responses related to scavenging reactive oxygen species in Ampelopsis species inoculated with Rhizobium vitis
    Hae In Lee, Zar Le Myint, Soon Young Ahn, Seung Heui Kim, Hae Keun Yun
    Horticulture, Environment, and Biotechnology.2023; 64(4): 655.     CrossRef
  • Anatomical and biochemical changes in leaves of Vitis labrusca L. cv. Niagara Rosada in response to infection by Elsinoë ampelina Shear
    Zélia Valente Braga, Larissa Fernanda Muniz, Gislene Roberta Manarim, Claudio Lima de Aguiar, Beatriz Appezzato-da-Glória
    Brazilian Journal of Botany.2021; 44(1): 187.     CrossRef
  • Identification and functional characterisation of an allene oxide synthase from grapevine (Vitis vinifera L. Sauvignon blanc)
    Walftor Dumin, Michael Rostas, Christopher Winefield
    Molecular Biology Reports.2018; 45(3): 263.     CrossRef
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Efficiency of Sequence Characterized Amplified Region Markers for Selecting Non-Astringent Persimmon (Diospyros kaki Thunb.)
Yeo-Ok Park, Hee-Jeong Jae, Ji-Young Shon, Seong-Tae Choi, Sung-Chul Kim, Yong-Cho Cho, Kwang-Pyo Hong, Younghoon Park
Plant Breed. Biotech. 2016;4(3):336-344.   Published online August 31, 2016
DOI: https://doi.org/10.9787/PBB.2016.4.3.336

Persimmon (Diospyros kaki Thunb.) is classified into four types based on the fruit traits, astringency and flesh color. Of the four types, the pollination-constant non-astringent (PCNA) fruit is typically most desirable for consumption. In the present study, we used five sequence characterized amplified region (SCAR) markers associated with astringency in persimmon fruit, namely E4/E9r, E4/A2r, 7H9F/AST-R, AST-F/AST-R, and AST-F/PCNA-F/5R3R, to improve the efficiency of PCNA-type persimmon breeding via marker-assisted selection (MAS). A total of 84 cultivars of the four types and their segregating F1 progeny were used to evaluate the association of SCAR markers with the fruit astringency phenotype. Polymerase chain reaction evaluation of each SCAR marker showed that E4/E9r combined with AST-F/PCNA-F/5R3R was appropriate for selecting the ast allele responsible for PCNA-type fruit, as the phenotype-genotype match percentages of these two markers were 94% and 99%, respectively. This MAS was verified by the successful use of AST-F/PCNA-F/5R3R to select 107 PCNA-type individuals from 609 F1 hybrid progeny derived from various crosses.

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Genetic Diversity and Population Structure of Rubus Accessions Using Simple Sequence Repeat Markers
Kyung Jun Lee, Gi-An Lee, Hee-Kyoung Kang, Jung-Ro Lee, Sebastin Raveendar, Myoung-Jae Shin, Yang-Hee Cho, Kyung-Ho Ma
Plant Breed. Biotech. 2016;4(3):345-351.   Published online August 31, 2016
DOI: https://doi.org/10.9787/PBB.2016.4.3.345

Sixty-nine Rubus accessions were analyzed to determine the genetic relationships using simple sequence repeat (SSR) markers. Twenty-three SSR markers generated a total of 351 alleles from all accessions, with an average of 15.3 alleles per locus. The average value of polymorphism information content was 0.76, ranging from 0.52 to 0.91. As a result of population structure analysis, 69 Rubus accessions of six Rubus species were subdivided into six subpopulations. Four subpopulations included distinct Rubus species accessions; pop2 (Rubus crataegifolius var. subcuneatus, 2 accessions), pop3 (R. crataegifolius Bunge., 18 accessions), pop4 (R. fruticosus L., 3 accessions) and pop6 (R. coreanus Miq., 36 accessions) while The pop1 and pop5 mainly included R. idaeus L. and R. parvifolius L., respectively. In cluster analysis, 69 Rubus accessions were divided into three groups. Group I contained 35 Rubus accessions, which consisted of six Rubus species. Groups II and III had 30 and 4 Rubus accessions, respectively. They consisted of only R. coreanus. The uncertain diversity of species and artificial groups of the Rubus genus has created confusion with respect to the correct classification of the species at both commercial and scientific levels. The results of the present study will provide basic information for phylogeny, taxonomy and breeding programs of Rubus species.

Citations

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  • Genetic diversity and population structure of some blackberry genotypes collected from different parts of Türkiye using inter simple sequence repeat (ISSR) markers
    Fatma Alan, Aydın Uzun, Hasan Pınar
    Genetic Resources and Crop Evolution.2025; 72(7): 9001.     CrossRef
  • Cross-transferability of Rubus ellipticus EST–SSR markers for genetic diversity analysis of peach (Prunus persica)
    Samriti Sharma, Rajinder Kaur, Krishan Kumar, Heerendra Sagar
    Genetic Resources and Crop Evolution.2024; 71(4): 1615.     CrossRef
  • Genetic diversity and population relationships in wild Korean black raspberry (Rubus coreanus Miq.) based on microsatellite markers: establishing a fruit tree breeding strategy
    Sung-Kyung Han, Hanna Shin, Jei-Wan Lee, Kyung-Nak Hong, Ji-Young Ahn
    Horticulture, Environment, and Biotechnology.2024; 65(2): 293.     CrossRef
  • Genetic differentiation between Czech and Norwegian raspberry populations: new options for breeding
    Jiří Sedlák, Leona Leišová-Svobodová, Inger Martinussen, Vojtěch Holubec
    Euphytica.2022;[Epub]     CrossRef
  • Genetic variability in Rubus ellipticus collections assessed by morphological traits and EST-SSR markers
    Samriti Sharma, Rajinder Kaur, Krishan Kumar, Dinesh Kumar, Amol Kumar U. Solanke
    Journal of Plant Biochemistry and Biotechnology.2021; 30(1): 37.     CrossRef
  • Evaluación de marcadores microsatélites (SSRs) heterólogos en Rubus niveus para estudios de diversidad genética en las Islas Galápagos
    Pablo Alarcón Bolaños, María de Lourdes Torres, Gabriela Pozo, María Paula Erazo, Mayra Ortega, Estefanía Rojas, Noelia Barriga, Antonio Leon Reyes
    ACI Avances en Ciencias e Ingenierías.2021; 13(2): 20.     CrossRef
  • Recent Large-Scale Genotyping and Phenotyping of Plant Genetic Resources of Vegetatively Propagated Crops
    Hilde Nybom, Gunārs Lācis
    Plants.2021; 10(2): 415.     CrossRef
  • MODERN WAYS OF RASPBERRY BREEDING
    L. V. FROLOVA, T. A. HASHENKO, O. A. HASHENKO
    Fruit-Growing.2021; 33: 211.     CrossRef
  • Genetic diversity of the Andean blackberry (Rubus glaucusBenth.) in Ecuador assessed by AFLP markers
    Patricia Garrido, Eduardo Morillo, Wilson Vásquez-Castillo
    Plant Genetic Resources: Characterization and Utilization.2020; 18(4): 243.     CrossRef
  • Molecular markers in the genetic diversity studies of representatives of the genus Rubus L. and prospects of their application in breeding
    A. M. Kamnev, O. Yu. Antonova, S. E. Dunaeva, T. A. Gavrilenko, I. G. Chukhina
    Vavilov Journal of Genetics and Breeding.2020; 24(1): 20.     CrossRef
  • Genetic and genomic resources for Rubus breeding: a roadmap for the future
    Toshi M. Foster, Nahla V. Bassil, Michael Dossett, Margaret Leigh Worthington, Julie Graham
    Horticulture Research.2019;[Epub]     CrossRef
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Cold Stress Evaluation among Maize (Zea mays L.) Inbred Lines in Different Temperature Conditions
Muhammad Qudrat Ullah Farooqi, Ju Kyong Lee
Plant Breed. Biotech. 2016;4(3):352-361.   Published online August 31, 2016
DOI: https://doi.org/10.9787/PBB.2016.4.3.352

Maize (Zea mays L.) is a crop in a tropical region which resists growing under sensitive temperature. This study was conducted to evaluate the performance of Canadian maize inbred lines under controlled cold stress conditions (5°C, 10°C, and 23°C). Data were recorded by measuring germination rate, index, root length, and seed vigour index values. Five higher and three lower tolerant inbred lines were shortlisted. The data were analyzed using analysis of variance, while mean values were compared using Tukey’s Honest Significant Difference Test at α=0.05 and at α=0.01. Using Genstat software, correlation was done. A strong correlation (P<0.05) was found between germination rate and germination index under all stress conditions. Root length and vigour index were also strongly correlated with germination rate under 5°C stress condition and compared to 10°C and 23°C stress conditions. Our results suggested that five (CO439, CO438, CO450, CO435, and CO445) among 22 maize inbred lines performed better under 5°C cold stress condition and thus had the potential to develop maize hybrids to increase grain yield under environmentally stressful conditions in South Korea.

Citations

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  • The Effect of Plasma-Activated Water on Zea mays L. Landraces Under Abiotic Stress
    Paula-Maria Galan, Silvia Strajeru, Danela Murariu, Catalin-Ioan Enea, Denisa-Elena Petrescu, Alina-Carmen Tanasa, Dumitru-Dorel Blaga, Livia-Ioana Leti
    Agriculture.2025; 15(19): 2037.     CrossRef
  • Low temperature elicits differential biochemical and antioxidant responses in maize (Zea mays) genotypes with different susceptibility to low temperature stress
    Salika Ramazan, Hilal Ahmad Qazi, Zahoor Ahmad Dar, Riffat John
    Physiology and Molecular Biology of Plants.2021; 27(6): 1395.     CrossRef
  • Genome-wide association mapping in maize: status and prospects
    Kumari Shikha, J. P. Shahi, M. T. Vinayan, P. H. Zaidi, A. K. Singh, B. Sinha
    3 Biotech.2021;[Epub]     CrossRef
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Chemical Constitutions and Antioxidant Activities of Tomato Leaf Extracts
Kyung Jun Lee, Gi-An Lee, Kyung-Ho Ma, Sebastin Raveendar, Yang-Hee Cho, Jung-Ro Lee, Jong-Wook Chung
Plant Breed. Biotech. 2016;4(3):362-372.   Published online August 31, 2016
DOI: https://doi.org/10.9787/PBB.2016.4.3.362

The present study aimed to determine the contents of five flavonols and two glycoalkaloids as well as the antioxidant activities of leaf ethanol extracts of 50 tomato accessions. The antioxidant activity was assessed using different tests: 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), 2,2-diphenyl-1-picryl hydrazyl (DPPH), nitric oxide (NO), and total polyphenol content (TPC). Flavonols and glycoalkaloids contents were determined using a high performance liquid chromatography-diode array detector system. Among five flavonols and two glycoalkaloids, naringenin and tomatine were detected in tomato leaves at high concentrations. Of the 50 tomato accessions, IT 229711, IT2365203, and IT 207224 were found to have the highest contents of quercetin, kaempferol, and tomatine, respectively. Leaf extract of IT189949 exhibited the highest relative antioxidant capacity index (RACI). Among the five flavonols, myricetin showed positive correlations with DPPH, ABTS, and NO, while isorhamnetin had positive correlation with DPPH. These results will expand the chemical constitution database and provide information on tomato leaves. They are valuable for the development of functional foods or feed-additives.

Citations

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  • Protective role of α-tomatine against oxidative stress induced reactive oxygen species: In vitro radical scavenging assays

    International Journal of Biosciences (IJB).2025; : 123.     CrossRef
  • Genetic Response of Solanum lycopersicum L. (Tomato) to Phytophthora infestans and Aspergillus niger
    Tavershima Moses Anakaa, Odunayo Joseph Olawuyi, Segun Gbolagade Jonathan
    International Journal of Plant Biology.2025; 16(1): 35.     CrossRef
  • Extracts of tomatoes and potatoes as biopesticides: a review
    Joshua Ibukun Adebomi, Jianfeng Guo, Catherine Hui Niu
    Discover Agriculture.2025;[Epub]     CrossRef
  • Solanaceae Leaves as are Sources of Antioxidants and Vitamin D
    Ekaterina Borodina, Oksana Kozlova, Veronika Boger, Larisa Proskuryakova, Vladimir Yustratov
    Food Processing: Techniques and Technology.2025; 55(1): 197.     CrossRef
  • Bioformulation development via valorizing silica-rich spent mushroom substrate with Trichoderma asperellum for plant nutrient and disease management
    Garima Singh, Abhay Tiwari, Akansha Gupta, Ashwani Kumar, P. Hariprasad, Satyawati Sharma
    Journal of Environmental Management.2021; 297: 113278.     CrossRef
  • Acute and Subacute Toxicity Profiles of the Methanol Extract of Lycopersicon esculentum L. Leaves (Tomato), a Botanical with Promising In Vitro Anticancer Potential
    Gaëlle S. Nguenang, Arsène S. M. Ntyam, Victor Kuete, Armando Zarrelli
    Evidence-Based Complementary and Alternative Medicine.2020;[Epub]     CrossRef
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Method and Technology
A Quick and Simple In-house Screening Protocol for Cold-Tolerance at Seedling Stage in Rice
Hasina Khatun, Partha S Biswas, Hung Goo Hwang, Kyung-Min Kim
Plant Breed. Biotech. 2016;4(3):373-378.   Published online August 31, 2016
DOI: https://doi.org/10.9787/PBB.2016.4.3.373

Cold stress is an emerging threat for rice production in Bangladesh particularly in Boro season (winter rice) at seedling stage. Cold stress during seedbed stage or early establishment stage at the main field induces severe seedling mortality that increases cost cultivation and delays crop establishment and ultimately entails into low yield. Development of sustainable cold tolerant high yielding rice varieties warrants an efficient and economic screening technique of germplasms and breeding population. The protocols for cold screening that so far have been used by the breeders and reported in literature are generally dependent on natural cool temperature and/or expensive climate chamber. In this paper, we report an in-house screening protocol that requires less than three weeks to complete the screening cycle and can be used all year round for mass screening of breeding population.

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