Glutinous rice is a key grain quality trait occupying an important part during rice processing in most rice growing areas. In this study, a gene pyramiding approach was used to introduce two dull genes, responsible for low amylose content, for glutinous rice breeding using marker assisted selection (MAS). The genotyping results revealed that rice cultivar Milyang319 carries both dull genes on chromosome 6, derived from cv. Milky-queen (Wx-mq) and cv. LGS-soft (du12(t)), respectively. Milyang319 had a significantly low amylose content (9.0%) compared to those observed in donor parents Milky-queen (15.7%) and LGS-soft (14.5%). In addition, the amylogram analysis of Milyang319 showed a similar pattern of glutinous rice. Originally, Milyang319 had a low hardness and more stickiness compared to those recorded in cooked rice of both dull parental lines. Therefore, Milyang319 is proposed as a promising candidate rice cultivar that could be used as a new source of waxy germplasms with same physicochemical features of glutinous rice in further breeding programs.

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• Improved Processing Quality in a tongil-type Waxy Rice Cultivar Associated with a Rare granule-bound starch synthase I Allele
  Seong-Gyu Jang, Ji-Min Kim, Ji-Yoon Lee, Jun-Hyeon Cho, Youngho Kwon, So-Myeong Lee, Jisu Choi, Ju-Won Kang, Jong-Min Jeong, Jong-Hee Lee, Dong-Soo Park, Soon-Wook Kwon, Sumin Jo
  Korean Journal of Breeding Science.2026; 58(2): 123.     CrossRef
• Molecular insights on the origin and development of waxy genotypes in major crop plants
  Vikram S Gaur, Salej Sood, Carlos Guzmán, Kenneth M Olsen
  Briefings in Functional Genomics.2024; 23(3): 193.     CrossRef
• Genetic control of grain appearance quality in rice
  Dongsheng Zhao, Changquan Zhang, Qianfeng Li, Qiaoquan Liu
  Biotechnology Advances.2022; 60: 108014.     CrossRef

Quantitative trait loci (QTLs) for the starch-related traits amylose content (AC) and resistant starch (RS) content have received much attention due to the potential benefits of grains high in these starch levels. In this study, QTLs associated with AC and RS content were identified using 92 recombinant inbred lines (RILs) developed from a cross between two closely related japonica cultivars ‘Dodamssal’ and ‘Hwayeong’. One QTL on chromosome 2 for RS content and 2 QTLs for AC on chromosomes 2 and 6 were detected. The F₂ population derived from a cross between Hwayeong and two selected RILs were used to analyze the interaction between starch branching enzyme 3 (SBE3) and granule-bound starch synthase 1 (GBSS1). The combined effect of SBE3 and GBSS1 in the F₂ population suggested that these two genes behaved in an additive manner in increasing AC. Haplotype analysis based on two SNPs in GBSS1 classified 117 rice accessions into three groups. At the first SNP site, all indica, Korean landrace, and weedy rice accessions had the Wx^a allele at the 5ʹ splice site of intron 1, whereas japonica accessions had the mutated Wx^b allele. This suggests that this splice-donor mutation is prevalent in japonica cultivars, but rare or absent in landrace and weedy rice cultivars. Landrace or weedy rice accessions harboring the Wx^a allele could be employed in breeding programs to manipulate AC in cultivated japonica rice considering the difficulty and time to introduce desirable indica traits into japonica due to reproductive barriers.

Citations

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• Improved Processing Quality in a tongil-type Waxy Rice Cultivar Associated with a Rare granule-bound starch synthase I Allele
  Seong-Gyu Jang, Ji-Min Kim, Ji-Yoon Lee, Jun-Hyeon Cho, Youngho Kwon, So-Myeong Lee, Jisu Choi, Ju-Won Kang, Jong-Min Jeong, Jong-Hee Lee, Dong-Soo Park, Soon-Wook Kwon, Sumin Jo
  Korean Journal of Breeding Science.2026; 58(2): 123.     CrossRef
• Evaluation of glycemic response and starch digestibility in Korean rice toward the development of low GI rice
  Chang-Min Lee, O-Young Jeong, Hyun-Su Park, Jeonghwan Seo, Songhee Park, Keon-Mi Lee, Mina Jin, Il-Ryong Choi, Jae-Hyuk Han
  Frontiers in Plant Science.2025;[Epub]     CrossRef
• Evolution and association analysis of SSIIIa in rice landraces of Yunnan Province
  Xia Li, Xiaomeng Yang, Li’e Yang, Hafiz Ghulam Muhu-Din Ahmed, Chunlian Yao, Jiazhen Yang, Luxiang Wang, Tao Yang, Xiaoying Pu, Yawen Zeng
  Biologia.2024; 79(7): 2203.     CrossRef
• Allelic Diversity at Protein Disulfide Isomerase Like 1-1 (PDIL1-1) Gene is Associated with Amylose Content in Japonica Rice
  Cheryl Adeva, Ju-Won Kang, Kyu-Chan Shim, Ngoc Ha Luong, Hyun-Sook Lee, Jong-Hee Lee, Sang-Nag Ahn
  Plant Breeding and Biotechnology.2023; 11(1): 56.     CrossRef
• Quality Characteristics of Rice-Based Ice Creams with Different Amylose Contents
  Gi-Un Seong, Ji-Yoon Kim, Jung-Soo Kim, Sae-Ul Jeong, Jun-Hyeon Cho, Ji-Yoon Lee, Sais-Beul Lee, Nkulu-Rolly Kabange, Dong-Soo Park, Kwang-Deog Moon, Ju-Won Kang
  Foods.2023; 12(7): 1518.     CrossRef
• Molecular Breeding of Zheyou810, an Indica–Japonica Hybrid Rice Variety with Superior Quality and High Yield
  Jian Song, Yongtao Cui, Honghuan Fan, Liqun Tang, Jianjun Wang
  Agriculture.2023; 13(9): 1807.     CrossRef
• Interaction of starch branching enzyme 3 and granule-bound starch synthase 1 alleles increases amylose content and alters physico-chemical properties in japonica rice (Oryza sativa L.)
  Kyu-Chan Shim, Cheryl Adeva, Ju-Won Kang, Ngoc Ha Luong, Hyun-Sook Lee, Jun-Hyeon Cho, HyunJung Kim, Thomas H. Tai, Sang-Nag Ahn
  Frontiers in Plant Science.2022;[Epub]     CrossRef
• QTL Mapping of Mineral Element Contents in Rice Using Introgression Lines Derived from an Interspecific Cross
  Cheryl Adeva, Yeo-Tae Yun, Kyu-Chan Shim, Ngoc Ha Luong, Hyun-Sook Lee, Ju-Won Kang, Hyun-Jung Kim, Sang-Nag Ahn
  Agronomy.2022; 13(1): 76.     CrossRef
• Evaluation of Hardness and Retrogradation of Cooked Rice Based on Its Pasting Properties Using a Novel RVA Testing
  Sumiko Nakamura, Junji Katsura, Yasuhiro Maruyama, Ken’ichi Ohtsubo
  Foods.2021; 10(5): 987.     CrossRef

Rice (Oryza sativa L.) germplasms with an amylose content above 30% were identified by screening 9481 accessions from the Rural Development Administration (RDA) gene bank. The total set of accessions came from 65 countries, including Korea, China, Japan, the Philippines, India, Taiwan, the United States, and Russia. High-throughput near infrared reflectance (NIR) spectroscopy equipped with a fiberoptic probe (700~2500 nm) was used to estimate the amylose content. The amylose contents ranged from 5 to 40% based on NIR spectroscopy; divided into 5% increments, the amylose content of 2820 accessions was found to be between 15 and 20%. To select rice accessions high in amylose, 239 accessions with an amylose content of 30% or greater were selected based on NIR spectroscopy data and cultivated in the field for final selection. Among the 239 accessions selected and cultivated, 151 were deemed agronomically satisfactory. Among the 151 accessions, based on laboratory analysis, 14 had an amylose content higher than 30% and 33 had an amylose content between 28 and 30%. The amylose contents of the reference cultivars Hopum-byeo and Sobi-byeo were 20.7% and 19.9%, respectively. Finally, successful selection of accessions with high amylose content from the large RDA gene bank collection was achieved based on a combination of NIR spectroscopy and laboratory data.

Citations

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• New Source of Rice with a Low Amylose Content and Slow In Vitro Digestion for Improved Health Benefits
  Pichayaporn Srinang, Sirimaporn Khotasena, Jirawat Sanitchon, Sompong Chankaew, Sanun Jogloy, Tidarat Monkham
  Agronomy.2023; 13(10): 2622.     CrossRef
• Quality Characteristics of Rice-Based Ice Creams with Different Amylose Contents
  Gi-Un Seong, Ji-Yoon Kim, Jung-Soo Kim, Sae-Ul Jeong, Jun-Hyeon Cho, Ji-Yoon Lee, Sais-Beul Lee, Nkulu-Rolly Kabange, Dong-Soo Park, Kwang-Deog Moon, Ju-Won Kang
  Foods.2023; 12(7): 1518.     CrossRef