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"Antimicrobial peptide"

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"Antimicrobial peptide"

Review Article

Application of Antimicrobial Peptides for Disease Control in Plants
Yu-Jin Jung, Kwon-Kyoo Kang
Plant Breed. Biotech. 2014;2(1):1-13.   Published online March 31, 2014
DOI: https://doi.org/10.9787/PBB.2014.2.1.001

A large number of antimicrobial peptides (AMPs) from different organisms have been characterized to date. AMPs are small molecular weight proteins with broad spectrum antimicrobial activity against bacteria, viruses, and fungi. Several diseases in plants could result in serious losses and decreasing quality and safety of agricultural products. Being an agent for plant defense, AMPs kill target cells through diverse mechanisms once in a target microbial membrane. Cathelicidins, defensins and thionins are the three major groups of epidermal AMPs in human and plants. Plant AMPs are structurally and functionally diverse and can be directed against other organisms, like herbivorous insects. The biological activity of plant AMPs primarily depends on interactions with membrane lipids. Several antimicrobial peptides have been expressed in transgenic plants to confer disease protection. Antimicrobial peptides are interesting compounds that can be efficiently exploited for disease control in plants in a way that complies with the strict regulations on the efficacy and safety of disease control strategy.

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Research Article

Soft rot disease caused by Pectobacterium carotovorum subsp. carotovorum (Pcc), is one of the most devastating diseases affecting the cruciferous plants industry worldwide. In our previous study, the soft rot-resistant transgenic Chinese cabbage (Brassica rapa L.) plants were produced via constitutively overexpressing a human cathelicidin antimicrobial peptide (hCAP18/LL-37). To unravel the molecular mechanisms underlying Pcc resistance of the transgenic plants, this study compares the global transcriptional profile of untransformed line (WT) and the transgenic lines (TG23, TG34) through hybridization with KBGP-24K, Chinese cabbage GeneChip. In total, 1,415 differentially expressed genes (DEGs) were identified, 910 of which were up-regulated, while 505 were down-regulated. The DEGs were classified into 31 categories after Gene ontology (GO) annotation, in which 68 genes are in response to stimulus and are involved in immune system process, 12 genes are related to cell wall, and 13 genes belong to transcription factors. These genes and those related to toxin and terpenoid metabolism, glutathione metabolism, biosynthesis of phenylpropanoids, and plant hormones were hypothesized to play major roles in the soft rot resistance of transgenic lines (TG23, TG34). Semiquantitative RT-PCR analysis showed that the transcript levels of several candidate genes in TG23 and TG34 were significantly higher than in WT both before and after Pcc inoculation, indicating their potential association with soft rot disease.

Citations

Citations to this article as recorded by  
  • Enhanced resistance to bacterial pathogen in transgenic tomato plants expressing cathelicidin antimicrobial peptide
    Yu-Jin Jung
    Biotechnology and Bioprocess Engineering.2013; 18(3): 615.     CrossRef
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