Elaeis guineensis is a tropical oil plant with the highest oil yield per unit area in the world. The Tenera hybrid is the most valuable variety for cultivation compared to the parent varieties Dura and Pisifera. It is difficult to select for the morphological characteristics of the oil palm cultivar in oil palm seedlings at the nursery stage; thus, the development of a molecular marker is necessary. In the present study, a sequence characterized amplified region (SCAR) marker was developed that yields 159-bp and 195-bp fragments specific for female and male parents, respectively. Sequence alignment revealed that the 159-bp fragment has a 36-bp deletion. Molecular characterization of the fragments reveals that the sequence is identical to the ALBINO3-like protein 2 (EgALB3.2) and is localized on chromosome 16 of the E. guineensis genome with expression noted in the kernel/endosperm of Tenera fruits only. These markers help in the selection of oil palm hybrids codominantly expressing both fragments; thus, heterozygous individuals can be distinguished from homozygous individuals. The SCAR-specific marker could therefore be used to distinguish oil palm hybrids from their parents by PCR. Moreover, these specific SCAR primers can be used directly to identify the oil palm hybrids without the need for postprocessing steps, and the specific fragments can be detected using an automated sequencer and real-time PCR. This marker-assisted selection is sensitive and suitable for the identification of oil palms in breeding programs.

Panax ginseng has been one of the most important herbal medicines used in Eastern Asia. Recently, various molecular markers have been developed to authenticate Panax species, but these markers cannot differentiate the exact varieties or variants of Korean ginseng cultivars. In this study, six cultivars of Korean ginseng (Chunpoong, Yunpoong, Gopoong, Gumpoong, Jakyung, and Hwangsook), P. quinquefolius, and P. notoginseng were differentiated by simple sequence repeat (SSR) marker development. Specific primer sets were designed for the 54 candidate sequences containing SSRs that were predicted. Finally, eight polymorphic SSR loci were developed. DNA fragment analysis was performed using fluorescence-labelled primers for the amplicons. Reproducibility tests were carried out using multiple samples of Korean ginseng cultivars and Panax species. Eight primer sets (PgSSR07, PgSSR08, PgSSR09, PgSSR17, PgSSR37, PgSSR40, PgSSR51, and PgSSR53) showing polymorphism were used for phylogenetic relationship analysis. Consequently, six Korean ginseng cultivars (Chunpoong, Yunpoong, Gopoong, Gumpoong, Jakyung, and Hwangsook), P. quinquefolius, and P. notoginseng could be identified using the combination of SSR markers discovered.

Citations

Citations to this article as recorded by  

• Development of Genomic SSR Markers to Reveal the Genetic Diversity in Trichosanthes kirilowii from Korea
  Jun-Su Kim, Raveendar Sebastin, Du-Hyun Baek, Yun-Sook Kim, Yong-Nam Cho, Doyun Kim, Tae-Young Heo, Jong-Wook Chung, Jae-Young Heo
  Molecular Biotechnology.2026; 68(6): 2927.     CrossRef
• Genetic diversity of Panax ginseng cultivated in Japan and its relation with some plant characteristics
  Honoka Ito, Michiho Ito
  Journal of Natural Medicines.2024; 78(1): 91.     CrossRef
• Development and authentication of Panax ginseng cv. Sunhong with high yield and multiple tolerance to heat damage, rusty roots and lodging
  Jiho Seo, Joon-Soo Lee, Sung-Lye Shim, Jun-Gyo In, Chol-Soo Park, Yong-Jae Lee, Hee-Jun Ahn
  Horticulture, Environment, and Biotechnology.2023; 64(5): 753.     CrossRef
• Comparative metabolic profiling of root, leaf, fruit, and stem tissues of Panax notoginseng
  Rui Shi, Bingjie Xiong, Shu He, Can Liu, Jiftah Ben-Asher, Abraham Rami Horowitz, Shu Wang, Xiahong He
  International Journal of Food Properties.2022; 25(1): 1132.     CrossRef
• Investigation of Morphological Characteristics and Genetic Diversity of Adenophora triphylla (Thunb.) A. DC. Using SSR Markers
  Dae Hui Jeong, Yurry Um, Yeong Bae Yun, Jeong Hoon Huh, Jiah Kim, Hong Woo Park
  Korean Journal of Medicinal Crop Science.2022; 30(6): 411.     CrossRef
• Development of Whole Genome Sequence-based Novel SSR Markers in Astragalus membranaceus (Fisch.)
  Mok Hur, Yurry Um, Yi Lee, Yoon Jeong Lee, Sung Cheol Koo, Woo Tae Park, Jang Hoon Kim, Yun Chan Huh, Youn Ho Moon
  Korean Journal of Medicinal Crop Science.2021; 29(6): 418.     CrossRef
• De novo assembly and Transcriptome characterization of an endemic species of Vietnam, Panax vietnamensis Ha et Grushv., including the development of EST-SSR markers for population genetics
  Dinh Duy Vu, Syed Noor Muhammad Shah, Mai Phuong Pham, Van Thang Bui, Minh Tam Nguyen, Thi Phuong Trang Nguyen
  BMC Plant Biology.2020;[Epub]     CrossRef
• Silicon foliage spraying improves growth characteristics, morphological traits, and root quality of Panax ginseng C.A.Mey
  Soo-won Jang, Nooruddin Bin Sadiq, Muhammad Hamayun, Jehyeong Jung, Taeksung Lee, Jung-Seok Yang, Bokyung Lee, Ho-Youn Kim
  Industrial Crops and Products.2020; 156: 112848.     CrossRef
• Genetic Diversity of Angelica gigas Nakai Collected in Korea using Genome-Wide SSR Markers
  Dae Hui Jeong, Yun Mi Park, Ki Yoon Kim, Hong Woo Park, Kwon Seok Jeon, Mahn Jo Kim, Jin Su Gil, Yi Lee, Yurry Um
  Korean Journal of Medicinal Crop Science.2019; 27(6): 376.     CrossRef
• Whole genome re-sequencing and development of SSR markers in oriental melon
  Woon-Ho Song, Sang-Min Chung
  Journal of Plant Biotechnology.2019; 46(2): 71.     CrossRef