Sorghum is the fifth most important grain crop worldwide. It is not only used as food and feed, but also as a resource for biofuel production. In addition, it has potential uses as a model plant for research on adaptation to environmental stress. In this study, mutant sorghum lines were generated by gammy ray irradiation. Ten of the M6 sorghum mutant lines were selected from 28 mutant lines on the basis of agronomic characteristics. These 10 lines, along with their original accessions/cultivar, were evaluated to determine the germination rate and the shoot and root length under salt treatment. Compared with their original accessions, three mutant lines (B5, SY6, and SY7) showed significant differentiation under saline conditions (150 mM NaCl), with increased shoot length (by 1.3-2.2 times) and root length (by 1.5-2.5 times). We determined the transcript levels of 20 abiotic stress-responsive genes in B5 (the most salt-tolerant mutant) and its original accession. These genes included those encoding heat shock proteins, aquaporins, ROS scavenging system, and transcription factors. In the B5 mutant, 15 genes showed differences in transcript levels between the control and the salt treatment. Salt treatment resulted in significant up-regulation of Sb03g045840 and down-regulation of Sb3g030750 in the B5 mutant. Here, we reported a simple method to identify genes related to salt tolerance in a sorghum mutant.
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Fragrance is considered one of the most highly valued grain quality traits in rice, yet its genetic basis among Philippine cultivars, especially traditional accessions, is unknown. This study characterized the fragrance gene Betaine aldehyde dehydrogenase isoform 2 (
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Global population is increasing day-by-day, simultaneously, crop production need to increase proportionately. Whereas, increase crop production being restricted due to abiotic and biotic stresses. Abiotic stresses are adversely affected crop growth and development, leading to crop loss globally and thereby causing huge amount of economic loss as well. Contrary, pathogens are attacked the plants imposing biotic stress and severely hampers the yield. Therefore, it is prime need to understand the molecular mechanism and genes involved to minimize the biotic and abiotic stresses for mitigating the
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Quantification of potato glycoalkaloids (PGA) exerting toxicity to humans has some limits if applied to potato breeding populations on a large scale due to its high cost and time consumption. The aim of this study was to investigate key genes involved in PGA biosynthesis and their tuber expression patterns in two potato cultivars, Atlantic with low PGA content (18.6 mg/100g FW) and Haryoung with high PGA content (40.1 mg/100g FW), and to test the utility of these PGA gene transcript levels as selectable markers in potato breeding program. Pot grown potato plants of both ‘Atlantic’ and ‘Haryoung’ were exposed to drought stress where the transcript accumulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase 1 (
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The differential expression of
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Cabbage (
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In this study, we isolated cDNA with open reading frame encoding putative methyl-binding domain proteins from red pepper, which was designated as
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