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"Lysine"

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"Lysine"

Research Article
Screening and Breeding for Biofortification of Rice with Protein and High Lysine Contents
Ji-Yun Lee, Ju-Won Kang, Su-Min Jo, Young-Ho Kwon, So-Myeong Lee, Sais-Beul Lee, Dong-Jin Shin, Dong-Soo Park, Jong-Hee Lee, Jong-Min Ko, Jun-Hyeon Cho
Plant Breed. Biotech. 2021;9(3):199-212.   Published online September 1, 2021
DOI: https://doi.org/10.9787/PBB.2021.9.3.199

A total of 134 domestic and foreign genetic resources were analyzed for their protein and amino acids contents in order to identify breeding lines with high lysine content to improve nutritional components of rice. The protein contents ranged between 6.7% and 14.8%, with an average of 8.7%. The Dharial mutant-derived lines had relatively high protein content with the highest amino acids content of 130.16 mg/g and the highest lysine content of 3.86 mg/g which is about 5 times higher than that of the parent. In the case of mutant-derived lines with high floury endosperm such as Goami2, Dodamssal, Milyang320 and LA1, the total amino acids content was as low as 72.61-82.0 mg/g; however, lysine content ranged high between 2.64-3.35 mg/g with lysine ratio to the total amino acids was 3.6%-4.1% which is higher than the average lysine content ratio of 3.0%. Furthermore, correlation analysis revealed a very strong positive correlation between the total amino acids and total protein contents. In contrast, lysine content showed no significant correlation neither with total amino acids nor with protein contents. The lysine content of Milyang320, which was grown in 5 regions across Korea was 0.33%, showing an increase of about 22% compared to that recorded in Nampyeong (0.27%). Therefore, these data suggest that lysine content of Milyang320 is controlled genetically and could be serve as a source in high lysine rice breeding program.

Citations

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  • Lysine Matters: Genetic and Biotechnological Innovations to Combat Protein Malnutrition
    Varinder Singh, Manjari Mishra, Sneh Lata Singla‐Pareek, Joy K. Roy, Ashwani Pareek
    Plant, Cell & Environment.2026; 49(3): 1509.     CrossRef
  • Assessing two decades of breeding for biofortified rice with zinc, iron or protein
    Gogineni S.V. Prasad, Chilukuri S. Rao, Kalambur Muralidharan, Ranganathan Sridhar, Ebrahim A. Siddiq
    Journal of Agriculture and Food Research.2025; 24: 102317.     CrossRef
  • Biofortification as a solution for addressing nutrient deficiencies and malnutrition
    Bindu Naik, Vijay Kumar, Sheikh Rizwanuddin, Sadhna Mishra, Vivek Kumar, Per Erik Joakim Saris, Naresh Khanduri, Akhilesh Kumar, Piyush Pandey, Arun Kumar Gupta, Javed Masood Khan, Sarvesh Rustagi
    Heliyon.2024; 10(9): e30595.     CrossRef
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    Chae-Min Han, Jong-Hee Shin, Sang-Kuk Kim, Jung-Gi Ryu
    Journal of Crop Science and Biotechnology.2023; 26(3): 359.     CrossRef
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  • Comparative Proteome-wide Characterization of Three Different Tissues of High-Protein Mutant and Wild Type Unravels Protein Accumulation Mechanisms in Rice Seeds
    Cheol Woo Min, Ravi Gupta, Ju-Young Jung, Randeep Rakwal, Ju-Won Kang, Jun-Hyeon Cho, Jong-Seong Jeon, Sun Tae Kim
    Journal of Agricultural and Food Chemistry.2023; 71(32): 12357.     CrossRef
  • GWAS to spot candidate genes associated with grain quality traits in diverse rice accessions of North East India
    Rahul K. Verma, S. K. Chetia, Vinay Sharma, Samindra Baishya, Himanshu Sharma, M. K. Modi
    Molecular Biology Reports.2022; 49(6): 5365.     CrossRef
  • Comparison of physicochemical properties of pollen substitute diet for honey bee (Apis mellifera)
    Hyun Jee Kim, Jinseok Hwang, Zakir Ullah, Bilal Mustafa, Hyung Wook Kwon
    Journal of Asia-Pacific Entomology.2022; 25(4): 101967.     CrossRef
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