Bread wheat (Triticum aestivum L.) is a fundamental crop worldwide, playing a crucial role in ensuring food security for human beings. Understanding the genetic diversity of wheat cultivars and developing genetic passports through DNA fingerprinting is essential for preventing varietal duplication enhancing breeding diversity and protecting breeders' rights globally. This study investigated the genetic diversity, phylogenetic relationships, and allelic frequencies of 32 Uzbekistan bread wheat cultivars using 66 polymorphic simple sequence repeat (SSR) markers. Unique marker alleles were identified and used to develop genetic passports for the cultivars. The results showed that the primers Barc181, Wmc522, Wmc407, Wms18, Cfa2201, Wms294, Cfd76, Wmc73, Wmc486, Cfa220, Wmc314, And Wms495 are reliable SSR markers for assessing genetic polymorphism in Uzbek wheat cultivars.

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