Flowering time (heading date) of the rice plant is considered an important agronomic trait for environmental adaptation and grain yield. It is controlled by multiple genes and is regulated by different environmental factors, such as day length, temperature, soil moisture, etc. So far, approximately 125 genes regulating flowering process and floral organ identity or development directly or indirectly have been reported in rice. Among these genes, Heading date 3a (
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Cold stress is an emerging threat for rice production in Bangladesh particularly in Boro season (winter rice) at seedling stage. Cold stress during seedbed stage or early establishment stage at the main field induces severe seedling mortality that increases cost cultivation and delays crop establishment and ultimately entails into low yield. Development of sustainable cold tolerant high yielding rice varieties warrants an efficient and economic screening technique of germplasms and breeding population. The protocols for cold screening that so far have been used by the breeders and reported in literature are generally dependent on natural cool temperature and/or expensive climate chamber. In this paper, we report an in-house screening protocol that requires less than three weeks to complete the screening cycle and can be used all year round for mass screening of breeding population.
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