Glutinous rice is a key grain quality trait occupying an important part during rice processing in most rice growing areas. In this study, a gene pyramiding approach was used to introduce two dull genes, responsible for low amylose content, for glutinous rice breeding using marker assisted selection (MAS). The genotyping results revealed that rice cultivar Milyang319 carries both dull genes on chromosome 6, derived from cv. Milky-queen (Wx-mq) and cv. LGS-soft (du12(t)), respectively. Milyang319 had a significantly low amylose content (9.0%) compared to those observed in donor parents Milky-queen (15.7%) and LGS-soft (14.5%). In addition, the amylogram analysis of Milyang319 showed a similar pattern of glutinous rice. Originally, Milyang319 had a low hardness and more stickiness compared to those recorded in cooked rice of both dull parental lines. Therefore, Milyang319 is proposed as a promising candidate rice cultivar that could be used as a new source of waxy germplasms with same physicochemical features of glutinous rice in further breeding programs.

Citations

Citations to this article as recorded by  

• Improved Processing Quality in a tongil-type Waxy Rice Cultivar Associated with a Rare granule-bound starch synthase I Allele
  Seong-Gyu Jang, Ji-Min Kim, Ji-Yoon Lee, Jun-Hyeon Cho, Youngho Kwon, So-Myeong Lee, Jisu Choi, Ju-Won Kang, Jong-Min Jeong, Jong-Hee Lee, Dong-Soo Park, Soon-Wook Kwon, Sumin Jo
  Korean Journal of Breeding Science.2026; 58(2): 123.     CrossRef
• Molecular insights on the origin and development of waxy genotypes in major crop plants
  Vikram S Gaur, Salej Sood, Carlos Guzmán, Kenneth M Olsen
  Briefings in Functional Genomics.2024; 23(3): 193.     CrossRef
• Genetic control of grain appearance quality in rice
  Dongsheng Zhao, Changquan Zhang, Qianfeng Li, Qiaoquan Liu
  Biotechnology Advances.2022; 60: 108014.     CrossRef

Advances in plant molecular techniques have dramatically widened the applicability of gene identification and pyramiding valuable genes. This study was carried out to pyramid five resistance genes for biotic stress into the japonica rice cultivar using marker-assisted selection (MAS) and marker-assisted background analysis of selected progenies using SSR markers. The Pi40, Xa4, xa5, Xa21 and Bph18 genes were combined in Jinbubyeo, a Korean japonica rice variety using MAS. Gene specific co-dominant PCR-based markers were used to select for homozygous recombinant lines in a segregating population derived from a cross between the parental homozygous resistant gene introgression lines. We had successfully developed multiple gene pyramided breeding lines (GPLs) for bacterial blight, blast, and brown planthopper using MAS in rice. The GPLs exhibited high resistance against biotic stress and had around 93% of the genetic background of the recurrent parent Jinbubyeo based on SSR graphical mapping. The yield and agronomic traits of the GPLs were similar to those of the recurrent parent, indicating that there is no apparent agronomic trait penalty associated with the presence of the resistance genes. The strategy of simultaneous foreground and phenotypic selection to introduce multiple R genes is very useful to reduce the cost and the time required for the isolation of desirable recombinants with target resistance genes in rice. The GPLs could be useful to enhance effective resistance for biotic stress and produce stable grain yield in japonica rice breeding programs.

Citations

Citations to this article as recorded by  

• Genetic Dissection of Resistance to Pseudomonas amygdali pv. tabaci in Soybean [Glycine max (L.) Merr.] by Linkage Analysis
  Seo Yoon Yang, In-Jeong Kang, Ji-Min Kim, Sungtaeg Kang, Sungwoo Lee
  The Plant Pathology Journal.2026; 42(2): 207.     CrossRef
• Grain quality characterization of indigenous rice (Oryza sativa L.) landraces using mixed clustering and selection index approaches
  A. K. Jukanti, S. Rathod, C. N. Neeraja, S. Gopala Krishnan, D. Aleena, G. Mahesh, G. Prasanna, R. M. Sundaram
  Discover Food.2025;[Epub]     CrossRef
• Genomic Confirmation of Resistance Genes for Blast, Bacterial Leaf Blight, Rice Tungro Spherical Virus, and Brown Planthopper in Tropically Adapted Temperate Japonica Rice Varieties
  Myrish Alvarez Pacleb, Seongkyeong Lee, Sherry Lou Hechanova, Thelma Padolina, Lenie Pautin, Jesson Del-Amen, Dong-Soo Park, Il-Ryong Choi, Sung-Ryul Kim, Dongjin Shin, Jung-Pil Suh
  Agronomy.2025; 15(11): 2585.     CrossRef
• Superior haplotypes towards the development of blast and bacterial blight-resistant rice
  Shamshad Alam, Krishna Tesman Sundaram, Uma Maheshwar Singh, Madamshetty Srinivas Prasad, Gouri Sankar Laha, Pallavi Sinha, Vikas Kumar Singh
  Frontiers in Plant Science.2024;[Epub]     CrossRef
• Toward Food Security in 2050: Gene Pyramiding for Climate-Smart Rice
  Isnaini Isnaini, Yudhistira Nugraha, Niranjan Baisakh, Nono Carsono
  Sustainability.2023; 15(19): 14253.     CrossRef
• Designing rice panicle architecture via developmental regulatory genes
  Ayumi Agata, Motoyuki Ashikari, Yutaka Sato, Hidemi Kitano, Tokunori Hobo
  Breeding Science.2023; 73(1): 86.     CrossRef
• Control of rice ratooning ability by a nucleoredoxin that inhibits histidine kinase dimerization to attenuate cytokinin signaling in axillary buds
  Yilong Yao, Denghao Xiang, Nai Wu, Yao Wang, Yu Chen, Yang Yuan, Ying Ye, Dan Hu, Chang Zheng, Yu Yan, Qingya Lv, Xiaokai Li, Guoxing Chen, Honghong Hu, Haiyan Xiong, Shaobing Peng, Lizhong Xiong
  Molecular Plant.2023; 16(12): 1911.     CrossRef
• Drought stress in rice: morpho-physiological and molecular responses and marker-assisted breeding
  Muhammad A. Hassan, Ni Dahu, Tong Hongning, Zhu Qian, Yi Yueming, Li Yiru, Wang Shimei
  Frontiers in Plant Science.2023;[Epub]     CrossRef
• Genetic Improvement of Rice for Bacterial Blight Resistance: Present Status and Future Prospects
  R. Abdul Fiyaz, D. Shivani, K. Chaithanya, K. Mounika, M. Chiranjeevi, G.S. Laha, B.C. Viraktamath, L.V. Subba Rao, R.M. Sundaram
  Rice Science.2022; 29(2): 118.     CrossRef
• Pyramiding of Four Broad Spectrum Bacterial Blight Resistance Genes in Cross Breeds of Basmati Rice
  Irfan Ullah, Hamid Ali, Tariq Mahmood, Mudassar Nawaz Khan, Muhammad Haris, Hussain Shah, Adil Mihoub, Aftab Jamal, Muhammad Farhan Saeed, Roberto Mancinelli, Emanuele Radicetti
  Plants.2022; 12(1): 46.     CrossRef
• Molecular Breeding of Water-Saving and Drought-Resistant Rice for Blast and Bacterial Blight Resistance
  Anning Zhang, Yi Liu, Feiming Wang, Deyan Kong, Junguo Bi, Fenyun Zhang, Xingxing Luo, Jiahong Wang, Guolan Liu, Lijun Luo, Xinqiao Yu
  Plants.2022; 11(19): 2641.     CrossRef
• A quantitative genomics map of rice provides genetic insights and guides breeding
  Xin Wei, Jie Qiu, Kaicheng Yong, Jiongjiong Fan, Qi Zhang, Hua Hua, Jie Liu, Qin Wang, Kenneth M. Olsen, Bin Han, Xuehui Huang
  Nature Genetics.2021; 53(2): 243.     CrossRef
• Identification of QTL Combinations that Cause Spikelet Sterility in Rice Derived from Interspecific Crosses
  Chang-Min Lee, Jung-Pil Suh, Hyun-Su Park, Man-Kee Baek, O-Young Jeong, Song-Joong Yun, Young-Chan Cho, Suk-Man Kim
  Rice.2021;[Epub]     CrossRef
• Mapping novel QTLs for yield related traits from a popular rice hybrid KRH-2 derived doubled haploid (DH) population
  Swapnil Ravindra Kulkarni, S. M. Balachandran, K. Ulaganathan, Divya Balakrishnan, A. S. Hari Prasad, G. Rekha, M. B. V. N. Kousik, S. K. Hajira, Ravindra Ramarao Kale, D. Aleena, M. Anila, E. Punniakoti, T. Dilip, K. Pranathi, M. Ayyappa Das, Mastanbee S
  3 Biotech.2021;[Epub]     CrossRef
• Recent Advances in Rice Varietal Development for Durable Resistance to Biotic and Abiotic Stresses through Marker-Assisted Gene Pyramiding
  Md Azadul Haque, Mohd Y. Rafii, Martini Mohammad Yusoff, Nusaibah Syd Ali, Oladosu Yusuff, Debi Rani Datta, Mohammad Anisuzzaman, Mohammad Ferdous Ikbal
  Sustainability.2021; 13(19): 10806.     CrossRef
• Effect of Resistance Genes on the Occurrence of Rice Undesirable Characters in a Wide Cross
  Chang-Min Lee, Hyun-Su Park, Man-Kee Baek, Jung-Pil Suh, O-Young Jeong, Song-Joong Yun, Suk-Man Kim
  Korean Journal of Breeding Science.2021; 53(4): 392.     CrossRef
• Molecular mapping of QTLs for yield related traits in recombinant inbred line (RIL) population derived from the popular rice hybrid KRH-2 and their validation through SNP genotyping
  Swapnil Ravindra Kulkarni, S. M. Balachandran, K. Ulaganathan, Divya Balakrishnan, M. Praveen, A. S. Hari Prasad, R. A. Fiyaz, P. Senguttuvel, Pragya Sinha, Ravindra R. Kale, G. Rekha, M. B. V. N. Kousik, G. Harika, M. Anila, E. Punniakoti, T. Dilip, S. K
  Scientific Reports.2020;[Epub]     CrossRef
• Impact of Marker Assisted Breeding for Bacterial Blight Resistance in Rice: A Review
  Hari Kesh, Prashant Kaushik
  Plant Pathology Journal.2020; 19(2): 151.     CrossRef
• Characterization and application of a gall midge resistance gene (Gm6) from Oryza sativa ‘Kangwenqingzhan’
  Yang Li, Yi Mo, Zhihua Li, Meng Yang, Lihua Tang, Ling Cheng, Yongfu Qiu
  Theoretical and Applied Genetics.2020; 133(2): 579.     CrossRef
• Marker Assisted Forward Breeding to Combine Multiple Biotic-Abiotic Stress Resistance/Tolerance in Rice
  Shilpi Dixit, Uma Maheshwar Singh, Arun Kumar Singh, Shamshad Alam, Challa Venkateshwarlu, Vishnu Varthini Nachimuthu, Shailesh Yadav, Ragavendran Abbai, Ramchander Selvaraj, M. Nagamallika Devi, Perumalla Janaki Ramayya, Jyothi Badri, T. Ram, Jhansi Laks
  Rice.2020;[Epub]     CrossRef
• Marker-assisted selection for grain number and yield-related traits of rice (Oryza sativa L.)
  Gayatri Gouda, Manoj Kumar Gupta, Ravindra Donde, Trilochan Mohapatra, Ramakrishna Vadde, Lambodar Behera
  Physiology and Molecular Biology of Plants.2020; 26(5): 885.     CrossRef
• Pyramiding Xa21, Bph14, and Bph15 genes into the elite restorer line Yuehui9113 increases resistance to bacterial blight and the brown planthopper in rice
  Chao He, Youlun Xiao, Jianghui Yu, Jinjiang Li, Qiucheng Meng, Xianguo Qing, Guoying Xiao
  Crop Protection.2019; 115: 31.     CrossRef
• Breeding Hybrid Rice with Genes Resistant to Diseases and Insects Using Marker-Assisted Selection and Evaluation of Biological Assay
  Me-Sun Kim, Sothea Ouk, Kuk-Hyun Jung, Yoohan Song, Le Van Trang, Ju-Young Yang, Yong-Gu Cho
  Plant Breeding and Biotechnology.2019; 7(3): 272.     CrossRef
• Insight into MAS: A Molecular Tool for Development of Stress Resistant and Quality of Rice through Gene Stacking
  Gitishree Das, Jayanta Kumar Patra, Kwang-Hyun Baek
  Frontiers in Plant Science.2017;[Epub]     CrossRef
• Rational design of high-yield and superior-quality rice
  Dali Zeng, Zhixi Tian, Yuchun Rao, Guojun Dong, Yaolong Yang, Lichao Huang, Yujia Leng, Jie Xu, Chuan Sun, Guangheng Zhang, Jiang Hu, Li Zhu, Zhenyu Gao, Xingming Hu, Longbiao Guo, Guosheng Xiong, Yonghong Wang, Jiayang Li, Qian Qian
  Nature Plants.2017;[Epub]     CrossRef