AT-hook proteins are known to co-regulate transcription of genes through the modification of chromatin architecture. In plants, many genes encoding AT-hook proteins have been shown to be associated with increased seed yield or delayed senescence. In this study, we produced transgenic soybean plants overexpressing chromatin architecture-controlling ATPG8 and ATPG10 genes by Agrobacterium-mediated transformation and examined their agronomic traits to identify the yield increase in soybean crop similar to those seen in model plants, Arabidopsis. A total of 16 (3 of pB2GW7.0-ATPG8 and 13 of pCSEN-ATPG10 transformed) transgenic soybean plants were produced and their T₁ seeds were harvested. Healthy and well-grown transgenic lines were selected (lines #1 and #2 from pB2GW7.0-ATPG8, and lines #8 and #9 from pCSEN-ATPG10), and the insertion and transcription level of genes were confirmed by PCR and RT-PCR with expected size. Investigation on agricultural traits confirms the increase in yield, plant height, the number of pods, and total seed weight with statistical significance when compared to wild-type soybean plants. The yield component study suggested that overexpression of ATPG8 and ATPG10 genes conferred positive effect on yield in transgenic soybean.

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  International Journal of Molecular Sciences.2022; 23(18): 10583.     CrossRef
• Comparative untargeted metabolomic analysis of Korean soybean four varieties (Glycine max (L.) Merr.) based on liquid chromatography mass spectrometry
  Eun-Ha Kim, Soo-Yun Park, Sang-Gu Lee, Hyoun-Min Park, Oh Suk Yu, Yun-Young Kang, Myeong Ji Kim, Jung-Won Jung, Seon-Woo Oh
  Journal of Applied Biological Chemistry.2022; 65(4): 439.     CrossRef
• Overexpression of ATHG1/AHL23 and ATPG3/AHL20, Arabidopsis AT-hook motif nuclear-localized genes, confers salt tolerance in transgenic Zoysia japonica
  Ha-Na Jeong, Hyeon-Jin Sun, Zhi-Fang Zuo, Dong Hee Lee, Pill-Soon Song, Hong-Gyu Kang, Hyo-Yeon Lee
  Plant Biotechnology Reports.2020; 14(3): 351.     CrossRef
• Increased Production of α-Linolenic Acid in Soybean Seeds by Overexpression of Lesquerella FAD3-1
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  Frontiers in Plant Science.2020;[Epub]     CrossRef

Metabolite profile is a powerful analytical technique to identify the functional characterization of plants. In this study, the phytochemicals and secondary metabolites of lentils (Lens culinaris) were analyzed to compare the anti-oxidative activities according to the different colors. The polar metabolites, fatty acids, carotenoids, flavonoids, anthocyanins, total phenolic acids, DPPH activity were analyzed. Three kind of lentils, French green whole lentil (FG), red whole lentil (LR), and green whole lentil (LG) (ASIA SEED Co., LTD), were used for this study. Fatty acids, phytochemicals, and antioxidative components from each lentil varieties were analyzed by official methods. The contents of lutein in carotenoids were 6–9 times higher than zeaxanthin in all lentils, but were not significantly different among three varieties. The content of carotenoids in FG was lower significantly than those in the LR and LG. Myricetin and luteolin were detected in the only FG. Kaempferol and delphinidin were significantly highest in the FG. Most of the phenolic acids except coumarate were higher in FG and LG than in LR. Also antioxidant effects (EC₅₀) were higher in FG and LG than in LR. The analyzed metabolites obtained from lentils showed distinct separation in the PCA results according to the varieties. Also, lentils showed different anti-oxidant profiles according to the colors. FG and LG showing higher contents of phytochemicals showed higher antioxidative activity than LG containing relative low contents of phytochemicals.

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  Inorganic Chemistry Communications.2026; 189: 116650.     CrossRef
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  Seyma Gunturk, Firuze Ergin Zeren, Ahmet Kucukcetin, Emine Mine Comak Gocer
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  Gıda.2026; 51(1): 1.     CrossRef
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  Asish Kumar Padhy, Shiksha Chaurasia, Abinaya Manivannan, Kuldeep Tripathi, Sapna Sapna, Sabhyata Bhatia
  Discover Food.2025;[Epub]     CrossRef
• Effect of soaking conditions on glyphosate absorption in selected pulses: understanding solvent behaviour and morphological changes
  Sindhu Sindhu, Annamalai Manickavasagan, Amanat Ali
  International Journal of Environmental Analytical Chemistry.2024; 104(19): 7855.     CrossRef
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  Minna Kahala, Lucia Blasco, Rina Bragge, Davide Porcellato, Hilde Marit Østlie, Thomas Rundberget, Jose Antonio Baz-Lomba, Juha-Matti Pihlava, Jarkko Hellström, Emilie Gullberg Jørgensen, Vesa Joutsjoki, Tove Gulbrandsen Devold, Anne Pihlanto
  LWT.2024; 208: 116691.     CrossRef
• Phenolic profile of whole seeds and seed fractions of lentils and its impact on antioxidant activity
  Antonio Manco, Carmela Gerardi, Giuseppe Romano, Leone D'Amico, Antonio Blanco, Francesco Milano, Gian Pietro Di Sansebastiano, Rind Balech, Barbara Laddomada
  Food Bioscience.2023; 54: 102887.     CrossRef
• Tissue-Specific Antioxidant Activities of Germinated Seeds in Lentil Cultivars during Thermal Processing
  Ji Hye Kim, Shucheng Duan, You Rang Park, Seok Hyun Eom
  Antioxidants.2023; 12(3): 670.     CrossRef
• Lentil (Lens culinaris Medik.) Flour Varieties as Promising New Ingredients for Gluten-Free Cookies
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  Foods.2022; 11(14): 2028.     CrossRef
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  Theresa Boeck, Lilit Ispiryan, Andrea Hoehnel, Aylin W. Sahin, Aidan Coffey, Emanuele Zannini, Elke K. Arendt
  Foods.2022; 11(14): 2013.     CrossRef
• Protective effects of functional foods against Parkinson's disease: A narrative review on pharmacology, phytochemistry, and molecular mechanisms
  Milad Iranshahy, Behjat Javadi, Amirhossein Sahebkar
  Phytotherapy Research.2022; 36(5): 1952.     CrossRef
• Genotypic and Environmental Effect on the Concentration of Phytochemical Contents of Lentil (Lens culinaris L.)
  Maria Irakli, Anastasia Kargiotidou, Evangelia Tigka, Dimitrios Beslemes, Maria Fournomiti, Chrysanthi Pankou, Kostoula Stavroula, Nektaria Tsivelika, Dimitrios N. Vlachostergios
  Agronomy.2021; 11(6): 1154.     CrossRef
• Nutritional and Rheological Features of Lentil Protein Isolate for Yoghurt-Like Application
  Theresa Boeck, Emanuele Zannini, Aylin W. Sahin, Juergen Bez, Elke K. Arendt
  Foods.2021; 10(8): 1692.     CrossRef
• Influence of partial pork meat replacement by pulse flour on physicochemical and sensory characteristics of low‐fat burgers
  Natalia Soledad Argel, Natalia Ranalli, Alicia Noemí Califano, Silvina Cecilia Andrés
  Journal of the Science of Food and Agriculture.2020; 100(10): 3932.     CrossRef
• LINKAGE MAPPING OF CAROTENOID CLEAVAGE DIOXYGENASE-4 FAMILY IN LENTIL GENOME
  Duygu Ates
  Turkish Journal Of Field Crops.2018; : 167.     CrossRef