Developing elite hybrid rice varieties is one important
objective
of rice breeding programs. Several genes related to male sterilities, restores, and pollinators have been identified through map-based gene cloning within natural variations of rice. These identified genes are good targets for introducing genetic traits in molecular breeding. This study was conducted to breed elite hybrid lines with major genes related to hybrid traits and disease/insect resistance in 240 genetic resources and F₁ hybrid combinations of rice. Molecular markers were reset for three major hybrid genes (S5, Rf3, Rf4) and thirteen disease/insect resistant genes (rice bacterial blight resistance genes Xa3, Xa4, xa5, Xa7, xa13, Xa21; blast resistance genes Pita, Pib, Pi5, Pii; brown planthopper resistant genes Bph18(t) and tungro virus resistance gene tsv1). Genotypes were then analyzed using molecular marker-assisted selection (MAS). Biological assay was then performed at the Red River Delta region in Vietnam using eleven F₁ hybrid combinations and two control vatieties. Results showed that nine F₁ hybrid combinations were highly resistant to rice bacterial blight and blast. Finally, eight F₁ hybrid rice varieties with resistance to disease/insect were selected from eleven F₁ hybrid combinations. Their characteristics such as agricultural traits and yields were then investigated. These F₁ hybrid rice varieties developed with major genes related to hybrid traits and disease/insect resistant genes could be useful for hybrid breeding programs to achieve high yield with biotic and abiotic resistance.

Citations

Citations to this article as recorded by  

• Resistance gene against Xanthomonas oryzae pv. Oryzae (Xoo) in rice: molecular mechanisms and breeding strategies for bacterial leaf blight
  Hongrui Jiang, Qina Huang, Changdeng Yang, Yan Liang
  Frontiers in Plant Science.2026;[Epub]     CrossRef
• Identification of new genetic resources for broad-spectrum blast resistance genes in Iranian rice germplasm
  Mostafa Modarresi, Hadis Shahbazi, Alireza Tarang, Farzin Pouramir, Maryam Hosseini Chaleshtori, Fatemeh Habibi
  Euphytica.2025;[Epub]     CrossRef
• ‘Drimi9ho’, A Lodging Tolerance with Mid-late Maturing, Improved White-backed Planthopper (Sogatella furcifera) and Cultivation Stability
  Jae-Ryoung Park, Eun-Gyeong Kim, Yoon-Hee Jang, Kyung-Min Kim
  Korean Journal of Breeding Science.2025; 57(4): 493.     CrossRef
• Origins of Susceptibility to Insect Herbivores in High-Yielding Hybrid and Inbred Rice Genotypes
  Finbarr G. Horgan, Maria Liberty P. Almazan, Carmencita C. Bernal, Christine Jade Dilla-Ermita, Goli Ardestani, Enrique A. Mundaca, Eduardo Crisol-Martínez
  Insects.2024; 15(8): 608.     CrossRef
• Heterosis for Interactions between Insect Herbivores and 3-Line Hybrid Rice under Low and High Soil Nitrogen Conditions
  Finbarr G. Horgan, Carmencita C. Bernal, Angelee Fame Ramal, Maria Liberty P. Almazan, Enrique A. Mundaca, Eduardo Crisol-Martínez
  Insects.2024; 15(6): 416.     CrossRef
• Genomic Architecture of Yield Performance of an Elite Rice Hybrid Revealed by its Derived Recombinant Inbred Line and Their Backcross Hybrid Populations
  Fan Zhang, Conghe Zhang, Xiuqin Zhao, Shuangbing Zhu, Kai Chen, Guixiang Zhou, Zhichao Wu, Min Li, Tianqing Zheng, Wensheng Wang, Zhi Yan, Qinyong Fei, Zhikang Li, Jinjie Chen, Jianlong Xu
  Rice.2022;[Epub]     CrossRef
• Genomic Approaches to Identify Molecular Bases of Crop Resistance to Diseases and to Develop Future Breeding Strategies
  Antonia Mores, Grazia Maria Borrelli, Giovanni Laidò, Giuseppe Petruzzino, Nicola Pecchioni, Luca Giuseppe Maria Amoroso, Francesca Desiderio, Elisabetta Mazzucotelli, Anna Maria Mastrangelo, Daniela Marone
  International Journal of Molecular Sciences.2021; 22(11): 5423.     CrossRef
• Genetic dissection of heterosis of indica–japonica by introgression line, recombinant inbred line and their testcross populations
  Wenqing Yang, Fan Zhang, Sundus Zafar, Junmin Wang, Huajin Lu, Shahzad Naveed, Jue Lou, Jianlong Xu
  Scientific Reports.2021;[Epub]     CrossRef
• Hybrid Incompatibility of the Plant Immune System: An Opposite Force to Heterosis Equilibrating Hybrid Performances
  Vanesa Calvo-Baltanás, Jinge Wang, Eunyoung Chae
  Frontiers in Plant Science.2021;[Epub]     CrossRef
• History and Results of Rice Breeding in Korea
  Young-Chan Cho, Man-Kee Baek, Hyun-Su Park, Jun-Hyun Cho, Eok-Keun Ahn, Jung-Pil Suh, Ji-Ung Jeung, Jong-Hee Lee, Yong-Jae Won, Yoo-Chun Song, Eung-Gi Jeong, Bo-Kyeong Kim, Jeom-Ho Lee
  Korean Journal of Breeding Science.2020; 52(S): 58.     CrossRef
• Transcriptional Modulation of Resistance against Xanthomonas oryzae pv. oryzae Korean Race K2 in japonica Rice
  Marjohn C. Niño, Yong-Gu Cho
  Agronomy.2020; 10(7): 960.     CrossRef

Abiotic and biotic stresses adversely affect rice (Oryza sativa L.) growth and yield. Conventional breeding is a very effective method to develop tolerant rice variety; however, it takes a decade long to establish a new rice variety. DNA-based markers have a huge potential to improve the efficiency and precision of conventional plant breeding via marker-assisted selection (MAS). The large number of quantitative trait loci (QTLs) mapping studies for rice has provided an abundance of DNA marker-trait associations. The limitations of conventional breeding such as linkage drag and lengthy time consumption can be overcome by utilizing DNA markers in plant breeding. The major applications of DNA markers such as MAS, QTL mapping and gene pyramiding have been surveyed. In this review, we presented the latest markers available for some of the most important abiotic and biotic stresses in rice breeding programs. Achieving a significant impact on crop improvement by marker assisted breeding (MAB) represents the great challenge for agricultural scientists in the next few decades.

Citations

Citations to this article as recorded by  

• Efficiency of doubled haploid technology in mining of multiple BB resistance genes from indica rice hybrid
  Sudhansu Sekhar Bhuyan, Byomkesh Dash, Prachitara Rout, Nupur Naik, Manjusha Chandravani, Nibedita Swain, Ram Lakhan Verma, Jawahar Lal Katara, Arup Kumar Mukherjee, C. Parameswaran, BN. Devanna, Snigdha Samir Pattnaik, Sanghamitra Samantaray
  Cereal Research Communications.2025; 53(3): 1319.     CrossRef
• The Development of Multi-Resistant Rice Restorer Lines and Hybrid Varieties by Pyramiding Resistance Genes against Blast and Brown Planthopper
  Zhao Deng, Peng Qin, Kaiyu Liu, Nan Jiang, Tianze Yan, Xuanwen Zhang, Chenjian Fu, Guangcun He, Kai Wang, Yuanzhu Yang
  Agronomy.2024; 14(5): 878.     CrossRef
• Emerging Trends in Wheat (Triticum spp.) Breeding: Implications for the Future
  Mujahid Alam, P. Stephen Baenziger, Katherine Frels
  Frontiers in Bioscience-Elite.2024;[Epub]     CrossRef
• Genomics and transcriptomics to protect rice (Oryza sativa. L.) from abiotic stressors: -pathways to achieving zero hunger
  Mushtaq Ahmad
  Frontiers in Plant Science.2022;[Epub]     CrossRef
• Advances from Conventional to Modern Plant Breeding Methodologies
  Sashi Lamichhane, Sapana Thapa
  Plant Breeding and Biotechnology.2022; 10(1): 1.     CrossRef
• Review on harnessing biotechnological tools for the development of stable bacterial wilt resistant solanaceous vegetable crops
  Shweta Sharma, Viveka Katoch, Devinder Kumar Banyal
  Scientia Horticulturae.2021; 285: 110158.     CrossRef
• Genotyping for Blast (Pyricularia oryzae) Resistance Genes in F2 Population of Supa Aromatic Rice (Oryza sativa L.)
  L. Kanyange, J. Kamau, O. Ombori, A. Ndayiragije, M. Muthini
  International Journal of Genomics.2019; 2019: 1.     CrossRef
• Breeding Hybrid Rice with Genes Resistant to Diseases and Insects Using Marker-Assisted Selection and Evaluation of Biological Assay
  Me-Sun Kim, Sothea Ouk, Kuk-Hyun Jung, Yoohan Song, Le Van Trang, Ju-Young Yang, Yong-Gu Cho
  Plant Breeding and Biotechnology.2019; 7(3): 272.     CrossRef
• Insight into MAS: A Molecular Tool for Development of Stress Resistant and Quality of Rice through Gene Stacking
  Gitishree Das, Jayanta Kumar Patra, Kwang-Hyun Baek
  Frontiers in Plant Science.2017;[Epub]     CrossRef
• Application and utilization of marker assisted selection for biotic stress resistance in hybrid rice (Oryza sativaL.)
  Jae-Young Song, Sothea Ouk, Franz Marielle Nogoy, Marjohn C. Niño, Soon Wook Kwon, Woongoo Ha, Kwon-Kyoo Kang, Yong-Gu Cho
  Journal of Plant Biotechnology.2016; 43(3): 317.     CrossRef