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"Zamin Shaheed Siddiqui"

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"Zamin Shaheed Siddiqui"

Research Article

Biochemical Changes of CaMsrB2 Expressing Transgenic Rice Seed during Germination in Heavy Metal Stress Environment
Zamin Shaheed Siddiqui, Kang Hyun Lee, Youn-Shic Kim, Gang-Seob Lee, Jung-Il Cho, Soo-Chul Park
Plant Breed. Biotech. 2019;7(3):287-294.   Published online September 1, 2019
DOI: https://doi.org/10.9787/PBB.2019.7.3.287

Biochemical changes of CaMsrB2 expressing transgenic rice seed during germination in heavy metal stress condition were studied. Transgenic lines, L-8 (single copy) and L-23 (two copy), along with WT were evaluated under metal stress conditions. All the plants were treated with different metals and their two selected concentration. Final germination rate, changes in amylase activity, total protein, reducing and total sugar was observed in all treated and control samples. Metal stress showed considerable impact on final germination rate in CaMsrB2 expressing transgenic rice seed. Application of lead salt showed 100% germination in L-23 compared to Zn and Cu. However, maximum germination rate was recorded in L-23 seed when it was treated with 4 mM PbCl2 and 0.5 mM CuCl2 compared to WT. Amylase activity and total reducing sugar was increased in transgenic rice seed treated with 2 mM and 4 mM PbCl2 as compared to WT. L-23 showed substantial increase in amylase activity and total reducing sugar compared to L-8 and WT. However, transgenic seeds treated with Zn and Cu showed substantial decreased in amylase activity and total reducing sugar with few exceptions. L-23 performed well regarding amylase activity and total reducing sugars in metal stress condition particularly in Pb as compared to Cu and Zn. CaMsrB2 expressing transgenic seed germination and their carbohydrate metabolism under metal stress condition were discussed. It was evident from the data that PbCl2 showed better germination rate due to enhance amylase activity and carbohydrate mobilization of CaMsrB2 expressing transgenic seed as compared to Cu and Zn.

Citations

Citations to this article as recorded by  
  • Chemometric study on the biochemical marker of the manglicolous fungi to illustrate its potentiality as a bio indicator for heavy metal pollution in Indian Sundarbans
    Shouvik Mahanty, Praveen Tudu, Somdeep Ghosh, Shreosi Chatterjee, Papita Das, Subarna Bhattacharyya, Surajit Das, Krishnendu Acharya, Punarbasu Chaudhuri
    Marine Pollution Bulletin.2021; 173: 113017.     CrossRef
  • Functional Characterization ofPsGPDin Drought Stress Response Using RNA-Seq Analysis of Transgenic Rice Plant
    So Young Kim, Hyemin Lim, Min Kang, Kyong Mi Jun, Seung Uk Ji, Soo-Chul Park, Gang-Seob Lee
    Plant Breeding and Biotechnology.2020; 8(2): 131.     CrossRef
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Review Article
Phenotyping of Plants for Drought and Salt Tolerance Using Infra-Red Thermography
Taek-ryoun Kwon, Kyung-hwan Kim, Hae-Jin Yoon, Seung-kon Lee, Beom-ki Kim, Zamin Shaheed Siddiqui
Plant Breed. Biotech. 2015;3(4):299-307.   Published online November 30, 2015
DOI: https://doi.org/10.9787/PBB.2015.3.4.299

Drought and salinity are the major environmental constrains in global agricultural production. Plant breeding for the drought and salt tolerance needs a proper assessment procedure to overcome stress constrain. Fundamental understanding on the physiological nature of the plant tolerance provides valuable information for the genetically modified crop’s development. Drought or salt stress induces several common physiological responses in plants such as water relation and photosynthetic capacitiy. It is because both stresses lead cellular dehydration in the plants, particularly, during the early phase of stress imposition. Drought and salinity decrease CO2 availability for photosynthesis via stomatal limitation as well as elevate leaf temperature due to partially closed stomata. In this scenario, stomatal regulation and plant water status are important aspects in abiotic stress environment. These physiological responses have a function to stabilize the temperature inside plant/leaf. Therefore phenotyping through an infra-red thermography (heat sensitive sensor), could be a useful tool in the selection of a tolerant genotypes. Infra-red thermography is a part of the electromagnetic spectrum which emits a certain amount of radiation as a function of their temperatures. In general, the plants which have less water, would have higher temperature and display more infra-red radiations. In abiotic stresses such as drought and salinity, plant water status is affected and varied from the sensitive to tolerant level. Infra-red images of plants are often linked with some of the physiological attributes to the tolerance. This review covers the limits, advantages, linkages, comparison and other prospectives of using thermal imagaes in modern phenotyping techniques.

Citations

Citations to this article as recorded by  
  • Artificial Intelligence (AI) in Detection of Abiotic Stress in Plants: A Review
    Anushree Matabber, Lionel Lami-Ndame Rhuhanga, Shinsuke Agehara, Maryam Mozafarian
    Sensors.2026; 26(4): 1122.     CrossRef
  • High throughput phenomics in elucidating drought stress responses in rice (Oryza sativa L.)
    S. Anand, R. L. Visakh, R. Nalishma, R. P. Sah, R. Beena
    Journal of Plant Biochemistry and Biotechnology.2025; 34(1): 119.     CrossRef
  • Functional phenotyping: Understanding the dynamic response of plants to drought stress
    Sheikh Mansoor, Yong Suk Chung
    Current Plant Biology.2024; 38: 100331.     CrossRef
  • Water and Nutrient Recovery for Cucumber Hydroponic Cultivation in Simultaneous Biological Treatment of Urine and Grey Water
    Anna Wdowikowska, Małgorzata Reda, Katarzyna Kabała, Piotr Chohura, Anna Jurga, Kamil Janiak, Małgorzata Janicka
    Plants.2023; 12(6): 1286.     CrossRef
  • Field identification of drought tolerant wheat genotypes using canopy vegetation indices instead of plant physiological and biochemical traits
    Pengfei Wen, Yu Meng, Chenkai Gao, Xiaokang Guan, TongChao Wang, Wei Feng
    Ecological Indicators.2023; 154: 110781.     CrossRef
  • Improving Drought Tolerance in Mungbean (Vigna radiata L. Wilczek): Morpho-Physiological, Biochemical and Molecular Perspectives
    Chandra Mohan Singh, Poornima Singh, Chandrakant Tiwari, Shalini Purwar, Mukul Kumar, Aditya Pratap, Smita Singh, Vishal Chugh, Awdhesh Kumar Mishra
    Agronomy.2021; 11(8): 1534.     CrossRef
  • Sustainable effect of a symbiotic nitrogen‐fixing bacterium Sinorhizobium meliloti on nodulation and photosynthetic traits of four leguminous plants under low moisture stress environment
    Z.S. Siddiqui, F. Ali, Z. Uddin
    Letters in Applied Microbiology.2021; 72(6): 714.     CrossRef
  • High-throughput phenotyping platform for analyzing drought tolerance in rice
    Song Lim Kim, Nyunhee Kim, Hongseok Lee, Eungyeong Lee, Kyeong-Seong Cheon, Minsu Kim, JeongHo Baek, Inchan Choi, Hyeonso Ji, In Sun Yoon, Ki-Hong Jung, Taek-Ryoun Kwon, Kyung-Hwan Kim
    Planta.2020;[Epub]     CrossRef
  • Thermal Imaging for Plant Stress Detection and Phenotyping
    Mónica Pineda, Matilde Barón, María-Luisa Pérez-Bueno
    Remote Sensing.2020; 13(1): 68.     CrossRef
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