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Review Article

Key Roles of Cysteine Protease in Different Plant Pathosystem

Plant Breeding and Biotechnology 2014;2(2):97-109.
Published online: June 30, 2014

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*Corresponding author: Yong-Gu Cho, ygcho@cbnu.ac.kr, Tel: +82-43-261-2514, Fax: +82-43-273-2242

Current Address, Philippine Rice Research Institute-Midsayap, North Cotabato 9410, Philippines

• Received: June 24, 2014   • Revised: June 26, 2014   • Accepted: June 27, 2014

Copyright © 2014 The Korean Society of Breeding Science

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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  • Cysteine protease is one of the well-studied proteolytic enzymes in plants. This class of protease has been implicated in various physiological aspects of developmental stages in plants including seed germination, senescence, and disease immunity. A handful of studies assign plants cysteine protease in different molecular battlefield under a few selected pathosystems, and initially extricate complex molecular mechanism of resistance. Interestingly, the same type of proteases released by pathogens have been demonstrated to play a crucial role in establishing disease infection in plants. Most of which target resistance proteins resulting either in an access to the hosts biochemical loot or cascades of signals for defense. As means of enabling and disabling host immunity, this class of protease is subject to diversifying selection, which is an intriguing outcome under natural host pathosystem. This paper summarizes the key roles of cysteine proteases in a few selected plant pathosystems.
Cysteine proteases activity comprises majority of the proteolytic activities in plants. It shares approximately 30% of total proteolysis in mature non-senescing organs, and in some cases rises to 90% in response to stimuli (Grudkowska and Zagdanska 2004). Papain-like cysteine protease (PLCP) is equipped with N-terminal auto-inhibitory domain covering the substrate binding groove which needs to be removed proteolytically for activation (Taylor et al. 1995). Cysteine proteases use catalytic cysteine residue as a nucleophile to cleave bonds of protein substrates (Shindo and van der Hoorn 2008) which results in an intermediate state where the enzyme is covalently attached to the substrate (van der Hoorn et al. 2004). Subsequent hydrolysis cleaves the peptide bond and releases the protease (Powers et al. 2002). The catalytic cysteine (C), which is responsible in binding with the substrate, is situated right in the middle of a cleft as part of a catalytic triad including histidine (H), and asparagine (N) (Drenth et al. 1971; Shindo and van der Hoorn 2008). Aside from catalytic residues, the protease domain also contains two disulfide bridges (SS) (Shindo and van der Hoorn 2008).
Apparently, plants utilize the same proteolytic machinery to halt pathogen invasion. There have been reports on papain-like cysteine proteases acting as inhibitory agent against fungus, bacteria, oomycete and nematode such as Rcr3 (Krüger et al. 2002), PIP1 (Tian et al. 2007), RD19 (Bernoux et al. 2008), RD21 (Shindo et al. 2012), C14 (Kaschani et al. 2010) and Cath B (Gilroy et al. 2007). Each confers unique participation in the induction of host immunity which is evident in the specific interactions with the effectors, involvement in a specific molecular battlefield, and the resulting immunity threshold. Interactions of proteases with their substrates and inhibitors, to some extent provokes selection of the counteracting inhibitors, non-cleavable substrates and other means to evade proteolysis (Shindo and van der Hoorn 2008) which lead to evolutionary diversification.
Interestingly, pathogens are also engaged in proteolysis which either leads to repression or activation of plant defense system. A substantial evidence revealed Pseudomonas syringae as among those which produce more than one family type of cysteine protease (CP) (Axtell and Staskawicz 2003; Mackey et al. 2003; Shao et al. 2003; Lopez-Solanilla et al. 2004; Coaker et al. 2005). Some of the reported microbial cysteine proteases are channeled into the apoplasts while others are directly translocated to the plant cytoplasm. Like other classes of effector, activities of PLCP effector also perturb key host resistance protein target.
This review focuses on some members of clans CA and CE that are related to plant disease and resistance. In particular, this paper shows the diverse functions of selected PLCPs such as Cath B, PIP1, Rcr3, RD19, RD21, PopP1, AvrPphB, and AvrRPT2. Moreover, this paper describes the nuclear trafficking incidence of PLCP during plant-pathogen interaction, and shows extent of evolutionary diversification of PLCP caused by selection pressure.
Major clan of cysteine proteases implicated to disease immunity
There are at least two important clans of cysteine proteases implicated to play crucial role in plant diseases and resistance, these include CA and CE. Both contain proteases with a papain-like fold (van der Hoorn 2008). MEROPS database showed that papain-like cysteine proteases (PLCPs) classified under CA are the most abundant in nature being present in numerous species of bacteria, archea, protozoa, fungi, plants, animals, and viruses (Rawlings et al. 2014). Most members of this clan are pre-proteases containing a signal sequence, an autoinhibitory prodomain, and a mature protease domain (van der Hoorn et al. 2004). To date, this clan comprises 32 families, four of which were indicated to be related to plant diseases and resistance including C1 (mainly host plants), C6, C58, and C70 (mainly pathogenic microbes) (Rawlings et al. 2014). The C1 family is subdivided into C1A, an extracytoplasmic PLCPs that possess disulfide bridges and accumulate in the vesicles, vacuole, and apoplast, and the subfamily C1B which comprises cytoplasmic PLCPs (subfamily C1B) (Rawlings et al. 2006; Shindo and van der Hoorn 2008). The MEROPS database has indicated 169 plants species with CP assigned to this family (Rawlings et al. 2014). Of which, 11 species were shown to contain more than 30 homologues in their genome including Glycine max, rice, Arabidopsis lyrata and Zea mays, among others.
The other three families under clan CA implicated to plant disease and immunity represent the key effectors important in initiating disease in a specific host plant. The C6 family contains cysteine endopeptidases of RNA viruses such as potato virus Y-type helper component peptidase (potato virus Y) and barley yellow mosaic virus-type helper component peptidase (Rawlings et al. 2014). The only known peptidase activity of this group is the cleavage at its own C-terminus, at a preferred sequence site-Tyr-Xaa-Gly+Gly-, which releases it from the viral polyprotein (Carrington and Herndon 1992; Rawlings et al. 2014). In the case of C58 family, endopeptidases members act as transamidases, which attach a lipid moiety to the exposed N-terminus of the substrate. This group is further classified to two subfamilies, C58A and C58B, both solely distributed among species of bacteria (Rawlings et al. 2014). The AvrPphB of Pseudomonas syringae is the only member of C58B family and has been implicated to initiate disease in beans (Shao and Dixon 2004). For family C70, putative members are mainly of bacterial origin showing close homology with AvrRpt2 peptidase in Pseudomonas syringae (Rawlings et al. 2014). AvrRpt2 has been demonstrated to induce self-cleavage as means of auto-activation, and inhibition of RIN4 protein in the host cell (Axtell et al. 2003). These activities contribute to the virulence of Pseudomonas syringae.
Several peptidases in clan CE exhibit preferential cleavage after diglycine due to the presence of aromatic residue such as tryptophan which follows the catalytic histidine in the sequence (Golubtsov et al. 2006; Rawlings et al. 2014). Proteases in this clan are characterized by a cysteine nucleophile and a catalytic core composed of three amino-acid residues, H/E/C or H/D/C (Hotson and Mudgett 2004). As mentioned in MEROPS database, members of CE contain two structural subdomains with the active site in the middle. One subdomain carries the active site His and Glu (or Asp) and the second subdomain carries the catalytic Cys. CE peptidases structure resembles clan CA except for the reversed order of subdomains. Peptidase family C55 comprises bacterial effector peptidases with restricted specificity, three of which are key factors affecting economically important crops. The members include PopP1 (Ralstonia solanacearum), AvrXv4 (Xanthomonas sp.) and HopZ1 protein (Pseudomonas syringae) (Rawlings et al. 2014).
Cysteine protease-mediated resistance in different pathosystem
Plants developed a variety of perception system such as pattern recognition receptors (PRRs) and resistance (R) gene products alerting the defense signals once perturbed by activities of pathogen. Classical definition of plant immunity solely involves ligand-receptor model indicating products of R genes as receptors that directly interact with the products of Avr genes (Keen 1990). However, in some R-Avr combinations, direct physical interactions are rather an isolated case as perception is often shown as indirect (van der Biezen and Jones 1998; Dangl and Jones 2001).
Cysteine proteases, particularly the PLCPs have been reported to play an important role either in pathogen perception taking the role as a guardee, and in disease resistance signaling (van der Hoorn 2008). Cysteine proteases are also involved in the programmed cell death (PCD) induced by developmental cues or pathogens (Solomon et al. 1999). The following are selected members of the PLCP acting in plant disease and resistance (Table 1).
Cath B
Hypersensitive response (HR) is a form of programmed cell death (PCD) that occurs at the site of pathogen entry and is mostly accompanied by the induction of defense responses to wall-off the pathogen (Lam et al. 2001). Using virus-induced gene silencing, Gilroy et al. (2007) demonstrated the involvement of cathepsin B in the plant disease resistance hypersensitive response. Effects of CathB-silencing to HR were demonstrated in two different non-host pathosystem involving N. benthamiana infiltrated with Erwinia amylovora (causal pathogen of fireblight in apple and pears) and Pseudomonas syringae pv. tomato (Pst) DC3000 (causal agent of bacterial speck in tomato). Both Eam- and Pst-mediated HR in TRV::NbCathB plants diminished even at the lower inoculum concentration (106 cfu ml−1) and the onset was delayed for 72 hpi at the higher concentration (107 cfu ml−1). This suppression leads to the accumulation of higher viable cells (an eight-fold increased recovery of Eam and a five-fold increase recovery of Pst) in virus-induced gene silencing (VIGS) plants than in control plants transformed with TRV::gfp. Using temporal expression analysis, regulation of the HR by CathB was validated wherein, expression of HR gene marker Hs203 was suppressed in VIGS plants. Interestingly, CathB also functions in HR triggered by a specific gene-for-gene interaction. The HR in TRV::gfp coexpressed with potato R3a and Phytophthora infestans Avr3a was abolished in TRV::NbCathB plants. But such suppression was not observed under Clasdosporium fulvum Avr4- and tomato Cf-4-mediated HR. The specificity of action suggests that Cath B is not an obligatory component for all PCD triggered during disease resistance. Detailed mechanism of resistance remains limited, nevertheless, these findings suggest the dynamic role of Cath B, being an important component of defense both in non-host and in specific gene-for-gene disease resistance. A detailed information was reported by Gilroy et al. 2007.
Rcr3
Recently, it has been shown that the Cf-2 mediates dual resistance in tomato involving two different pathogens, fungus and nematode, utilizing a common virulence target cysteine protease (Lozano-Torres et al. 2012). In the first report, a trans-membrane protein with extracellular leucine-rich repeats (LRRs) and short (23 to 36 amino acid) cytoplasmic domains-encoding Cf-genes confer resistance to the fungus Cladosporium fulvum in association with the required for Cladosporium resistance 3 (Rcr3) (Fig. 1B) (Dang and Jones 2001; Dixon et al. 2000; Krüger et al. 2002). Rcr3 encodes an apoplast-secreted papain-like cysteine protease of 344 amino acids with proven proteolytic activity (van der Hoorn 2008). Avr2 on the other hand encodes a secreted, cysteine-rich protein (Dixon et al. 1996). Inoculation of tomato with C. fulvum results in a rapid elevation of Rcr3 transcripts especially in incompatible (resistant) interactions, resembling a pathogenesis-related genes response (de Wit et al. 1986; Krüger et al. 2002). The resistance response involves HR at the site of inoculation thereby limiting further pathogen invasion (van der Hoorn 2008). Since Rcr3 is a secreted cysteine protease and has a specific role in Cf-2-mediated resistance, it was thought to function upstream of Cf-2 and was considered as obligatory component required for Cf-2-mediated fungal resistance (Krüger et al. 2002; Rooney et al. 2005). It was proposed that inhibition of Rcr3 by Avr2 induces a conformational change in Rcr3 resulting in Rcr3-Avr2 complex which activates Cf-2-mediated HR (Rooney et al. 2005; Bernoux et al. 2008). The mechanism pattern is consistent with the guard theory where in Cf-2 guards Rcr3 and triggers defense response upon perception of an Rcr3/Avr2 complex. A detailed information was reported by Krüger et al. 2002.
Interestingly, a recent study conducted by Lozano-Torres et al. (2012) demonstrates that the Cf-2-mediated resistance against the nematode Globodera rostochiensis requires the same target cysteine protease inhibited by the Avr2 from C. fulvum (Fig. 1C). As the obligate dormant period of the potato cyst nematode G. rostochiensis culminates and as soon as the nematode begins to hatch from the egg, its effectors are activated and are ready for translocation to the apoplast and cytoplasm of host cells through the stylet. Using liquid chromatography-mass spectrophotometry, the venom allergen-like effector protein, VAP1, was shown to be secreted to the apoplast of host cells alongside plant cell wall modifying proteins. Based on the yeast two-hybrid analysis which used Gr-VAP1 as bait, this effector was found to interact with the apoplastic cysteine protease Rcr3pim of tomato. A subsequent co-immunoprecipitation assay asserts the specificity of the Gr-VAP1 and Rcr3pim interaction. Disease screening for resistance to G. rostochiensis revealed a 68% lower infection in Cf-2/Rcr3pim compared to Cf-O/Rcr3-3 and Cf-2/rcr3-3 indicating that both cf-2 and Rcr3 are necessary for resistance to the cyst nematode in tomato. Further, transient expression of Gr-VAP1 in the leaves of tomato triggers a Cf-2/Rcr3pim-dependent programmed cell death. A detailed information was reported by Lozano-Torres et al. 2012.
PIP1
Phytophthora inhibited protease 1 (PIP1), a papain-like extracellular cysteine protease, is closely related to the tomato cysteine protease Rcr3 in the C1A-5 group (Tian et al. 2007). Genetic mapping analysis of the two genes in a population derived from a cross between tomato cv. Sun1642 and S. pimpinellifolium accession LA1589 reveals tight linkage of PIP1 and Rcr3 near marker TG14 on chromosome 2 (van der Knaap and Tanksley 2001; Tian et al. 2007). The oomycete P. infestans has been reported to induce late blight in tomato and potato (Birch and Whisson 2001; Smart and Fry 2001; Ristaino 2002). Motif searches of P. infestans unigenes revealed a family of cystatin-like protease inhibitors, two of which EPIC1 and EPIC2 were upregulated during infection of tomato (Tian et al. 2007). The Epic-PIP1 interaction was investigated by Tian et al. (2007). By performing coimmunoprecipitation assay on tomato intercellular fluids isolated from two different genotypes: Rcr3pim and rcr3 mutant, both incubated with rEPICs, it was found that EPIC2B interacts with PIP1 (Fig. 1E). Confirmation of EPIC2B-PIP1 interaction was done through western blot analysis of the intercellular fluids of PIP1-transiently transformed N. benthamiana. Results of cysteine protease activity profiling assay corroborated previous findings that EPIC2B inhibits the intercellular-secreted PIP1, a functional cysteine protease. Previously, PIP1 was shown to correlate with a large number of SA-induced PR protein genes including PR1, PR2, PR3, and PR7 (Zhao et al. 2003). Indeed, upregulation of PIP1 was observed during treatment with SA analog BTH implying that the gene is a PR protein. A detailed information was reported by Tian et al. 2007.
RD19
Most Gram-negative bacteria utilize type-III secretion system either to suppress basal defense or initiate effector-triggered immunity. This group of effectors is mostly translocated directly to the host cell cytoplasm (Jones and Dangl 2006). Ralstonia solanacearum is a soil-borne proteobacterium known to cause wilts especially to Solanaceous plants (Hayward 1991). R. solanacearum encodes Pseudomonas outer protein P2 (PopP2) which confers a specific immunity response in Arabidopsis mediated by RESISTANT TO R. SOLANACEARUM I-R (RRS1-R), its cognate R protein (Fig. 1A) (Deslandes et al. 2003). PopP2 is a cysteine protease effector and a member of YopJ/AvrRxv protein group under C55 peptidase family of clan CE (Barret and Rawlings 2001). RRS1-R, on the other hand, is an atypical resistance protein which contains Toll/Interleukin 1 receptor (TIR)-NBS-LRR domain and C-terminal WRKY domain (Deslandes et al. 2002; Eulgem and Somssich 2007). To investigate the components of RRS1-R/PopP2-mediated disease resistance signaling, Bernoux et al. (2008) performed a yeast two-hybrid with PopP2 as bait which led to the identification of RESPONSIVE TO DEHYDRATION19 (RD19), a predicted PLCP of subfamily C1A (Rawlings et al. 2014). Using gene knockout strategy, involvement of RD19 in RRS1-R-mediated resistance signaling was studied. Disease screening test involving mutant line (rd19/RRS1-R) showed compromised resistance wherein disease symptoms correlated with an increase of bacterial growth in the mutant. Confirmation of this function was done through genetic complementation of the mutant lines which resulted in a regained resistance phenotype. Accumulation of RD19 transcripts was found to increase after inoculation with R. solanacearum. The quantitative noninvasive FLIM approach reveals the physical association of RD19 with PopP2. Interestingly, it fails to show interaction between RRS1-R and RD19. A detailed information was reported by Bernoux et al. 2008.
RD21
The role of C14-like protease in the model pathosystem Hyaloperonospora arabidopsis (Hpa) and Arabidopsis thaliana has been investigated. Hpa is an obligate biotrophic oomycete pathogen causing downy mildew in Arabidopsis. It proliferates in the leaf apoplast and uses haustoria to gain access for nutrition in the parenchyma cells (Coates and Beynon 2010). Shindo et al. (2012) identified RD21 (At1g47128), the closest ortholog of tomato C14 with 58% prodomain homology, 76% protease domain similarity, and are 61% alike in granulin domain. Accordingly, a search for EPIC orthologs from Hpa was done and three EPIC-like proteins, HpaEPIC-A, -B and –C were identified. Phylogenetic analysis determined HpaEPIC1C-B and –C, the most likely ortholog of PinfEPIC1 and -2B, which were then selected for the subsequent analysis. To determine the function of the gene, knockout lines were generated. Subsequent disease evaluation shows that the gene knockout does not affect interactions with either virulent or avirulent strains of Hpa. To demonstrate the proposed role of the gene during incompatible interactions, another pathogen P. syringae was used to infect the mutant line, and yet the treatment failed to show changes in the phenotypic response compared with the wild type. The rd21 mutant was tested against the pathogen Botrytis cinerea and surprisingly it exhibits an increased susceptibility suggesting its specific role in immunity against necrotrophic fungal pathogen (Fig. 1F). This sensitivity response of the host proteases is in consistent with the fact that the B. cinerea genome does not encode PLCPs inhibitor protein (Rawling et al. 2010). A detailed information was reported by Shindo et al. 2012.
Cysteine protease secreted by phytopathogen
Pathogens have developed arsenal effectors that target host defense either by inhibiting or manipulating the receptors or host resistance gene products. Phytopathogenic bacteria use the type-III secretion system (TTSS) to directly deliver effector proteins into the plant cells presumably to initiate disease infection (Cornelis and Gijsegem 2000). These effector proteins are host-specific and trigger immunity in plants harboring a corresponding resistance gene (Keen 1990). Most Gram-negative phytobacteria contain TTSS that are encoded by hrp (hypersensitive response and pathogenicity) genes, proving their crucial role in mediating diverse plant-bacteria interactions (Lindgren et al. 1986; Alfano and Collmer 2004; He et al. 2004; Buttner and Bonas 2006). Previously, bacterial cysteine proteases have been shown to play important role both in compatible and incompatible host-gene interaction by targeting specific plant substrates (Hotson and Mudgett 2004). Most phytopathogenic bacterial cysteine proteases are classified to two major clans, CA and CE (Rawlings et al. 2014). The following are examples of cysteine protease TTSS in phytobacteria.
PopP1
Pseudomonas outer protein P1 (PopP1), a cysteine protease secreted by Ralstonia solanearum, is closely related with the YOPP/YOPJ pathogenicity factors from human pathogen Yersinia (Orth et al. 2000; Orth 2002). PopP1 encodes a 41-kDa protein and possesses the conserved amino acid triad characteristics for cysteine proteases (Orth et al. 2000). Questions regarding its function in plant-bacteria interaction were addressed by Lavie et al. (2002). The loss-of-function analysis revealed that PopP1 acts as a host-specificity factor encoding an Avr protein which modulates aggressiveness of the bacterium toward the host plant. Modulation of virulence is evident in the virulent nature of popp1 mutant in resistant petunia line St40, and in the restoration of avirulence following genetic complementation. Results in western blot using bacterial cells of wild type and the mutants showed that PopP1 is secreted by R. solanacearum using TTSS. But unlike PopA, PopB, and PopC TTSS-dependent effectors, PopP1 gene does not colocalized in the vicinity of the hrp gene cluster on the megaplasmid but rather on the bacterial chromosome. A detailed information was reported by Lavie et al. 2002.
AvrPphB
P. syringae pv. Phaseolicola secrets AvrpPhB, an avirulence protein responsible for initiating halo-blight disease in bean (Jenner et al. 1991). AvrPphB protein is a cysteine protease member of YopT family, capable of autoproteolytic cleavage of an AvrpPHB precursor for eliciting HR (Shao et al. 2002). Shao et al. (2002) demonstrated that proteolytic inactive mutants of AvrPphB (induced mutation in the AvrPphB catalytic triad C98S, H212A, and D227A) are incapable of inducing HR in Arabidopsis suggesting that proteolytic activity is needed for AvrPphB to induce HR. P. syringae strains carrying AvrPphB induce an HR when inoculated into Arabidopsis plants carrying the cognate R gene RPS5, and also, it induces HR when transiently expressed in tobacco leaves (Simonich and Innes 1995). Since HR cannot proceed without AvrPphB, it was proposed that RPS5 is activated after it binds to a peptide released from the substrate of AvrPphB (Shao et al. 2002). PBS1 kinase was considered as the potential substrate interacting with AvrPphB, as deletion of the PBS1 from Arabidopsis makes AvrPphB unrecognizable by RPS5 (Swiderski and Innes 2001). Further, AvrPphB was shown to cleave PBS1 independent of RAR1 and RPS5 in Arabidopsis (Shao et al. 2003). Specifically, this team of investigators identified the cleavage sites in PBS1 at a position composed of stretch of three amino acids immediately preceding the AvrPphB autocleavage site after K243. A detailed information was reported by Shao et al. 2003.
AvrRpt2
More recently, apart from the two sequence-unrelated TTSS proteins of P. syringae targeting Arabidopsis protein RIN4 (Mackey et al. 2002), a third sequence-unrelated effector called AvrRpt2 was found to inhibit RIN4 inside the host cell by inducing post transcriptional elimination (Mackey et al. 2003). AvrRpt2, a Pseudomonas syringae pv. tomato (Pst) TTSS effector protein, is thought to elicit an immunity response in RPS2-expressing Arabidopsis (KunKel et al. 1993). Translocation of AvrRpt2 to the cell during infection results in its cleavage at amino acids 71 and 72 to liberate a stable terminal 21 kDa product for RPS2 binding (Mudgett and Staskawicz 1999). Hotson and Mudgett (2004) demonstrate that activation of the RPS2 R protein solely depends on the AvrRpt2-mediated removal of RIN4 (Fig. 1D). In a proposed model by Mackey et al. (2003), inhibiting RIN4 disappearance prevents RPS2 activation, and the lethality associated with RIN4 disruption requires RPS2. Even at a barely detectable RIN4 levels, plants still initiate normal RPS2-dependent response to AvrRpt2 implying that only by total elimination of RIN4 does AvrRpt2 activate RPS2 (Mackey et al. 2002). Moreover, elaboration of AvrRpt2 interference mechanism was depicted in the physical interactions of AvrRpt2, RIN4, and RPM1 at the plasma membrane (Hotson and Mudgett 2004). A detailed information was reported by Mackey et al. 2002.
Nucleocytoplasmic shuttling of PLCP during interaction
Recent studies on subcellular localization of key resistance components situate nucleus at the forefront of the host-pathogen interface (Rivas 2012). With the accumulating evidence highlighting the significance of nuclear localization of effectors and key host resistance components for disease resistance in plants, nuclear dynamics is taken as a key event essential for plant defense (Deslandes and Rivas 2011). Some of the dynamic cellular processes that occur with defense response include plant cell trafficking, cytoskeleton reorganization, organelle positioning, and cytoplasmic-nuclear transport and partitioning (Lipka and Panstruga 2005; Robatzek 2007; Shen et al. 2007; Shen and Schulze-Lefert 2007; Wiermer et al. 2007; Bernoux et al. 2008). Among the interesting consequences of nuclear translocation of effectors is the subcellular localization of their cognate R proteins (Deslandes et al. 2003; Shen et al. 2007). Having been implicated in different pathosystems, PLCPs were shown to be an important target of microbial effectors resulting in nucleocytoplasmic trafficking during interaction.
PopP2 from R. solanacearum presents a nuclear localization signal (NLS) that is required for its nuclear translocation (Deslandes et al. 2003). As previously reported, direct translocation of PopP2 effector to the nucleus of host plant cells promotes nuclear accumulation of RRS1-R, an atypical R protein which confers resistance to R. solanacearum, possibly by preventing its proteasomal degradation (Deslandes et al. 2003; Rivas 2012). RRS1-R has been shown to interact with its cognate effector PopP2 in yeast cells (Deslandes and Rivas 2011). PopP2 interaction with RRS1-R was proposed to regulate defense-related gene expression either directly via the RRS1-R WKRY domain or through the action of additional plant TFs (Deslandes et al. 2003; Tasset et al. 2010). In their extensive study, Bernoux et al. (2008) identified and demonstrated additional important component of PopP2-RRS1-R mediated resistance. By performing yeast-two hybrid screening using PopP2 as bait, a cysteine protease RD19 was identified among the targets. Subsequent experiments assert that RD19 is a crucial host factor for PopP2-triggered RRS1-R-mediated resistance as exemplified in rd19 loss of function and genetic complementation studies. As detailed by Bernoux et al. (2008), RD19 localizes to the lytic vacuole and becomes active after proteolysis (Paris et al. 1996). Interestingly, when co-expressed in N. benthamiana, RD19 was shown to shuttle to the nucleus with PopP2. Further, the FLIM analysis in living cells confirmed a physical interaction between PopP2 and RD19 in the nucleus (Fig. 1A). Intriguingly, there was no interaction detected between Rd19 and RRS1-R. Also, PopP2 and RD19 association was not affected in the presence of RRS1-R. Since RD19 is required for Arabidopsis resistance to Ralstonia, it was proposed that the RD19-PopP2 nuclear complex is the one required for activation of the plant resistance response (Rivas 2012). How RD19 exactly activates resistance response remains unknown but Bernoux et al. (2008) theorized that the nuclear RD19 may function as transcriptional activator or competes with RRS1-R for similar or overlapping cis-elements in the promoters of defense-related genes. Unlike PopP2, RD19 lacks consensus NLS that would have allow its nuclear trafficking (Rivas 2012). Possibly, the vacuolar membrane collapse during the elicitor-induced membrane permeabilization releasing RD19 from vacuole-associated compartments into the cytoplasm where they may become available for SUMOylation (Matarasso et al. 2005; Bernoux et al. 2008), a process which generate signals required for their nuclear translocation (Rivas 2012). A detailed information was reported by Bernoux et al. 2003.
Coevolutionary arms race between plants PLCP and effectors
Translocation mechanisms for pathogen effectors are continuously evolving as means of manipulating the host cytoplasm and suppression of host immunity (Jones and Dangl 2006). Selection pressure on host enzyme yields variant residues at the interaction surface of the enzyme-inhibitor interactions (Misas-Villamil and van der Hoorn 2008). An emerging concept in antagonistic host-pathogen interactions states that to escape manipulation, effector/avirulence targets are subject to diversifying selection (Hougenhout et al. 2009). Shabab et al. (2008) reported that tomato plants create a harmful proteolytic apoplast through secretion of PLCPs during defense to halt pathogen build up. In particular, tomato apoplast is populated with remarkable diverse PLCP activities by seven PLCPs that fall into four different subfamilies, two of which are PIP1 and RCR3. However, Avr2 effector inhibits all apoplastic PLCP activities. Shabab et al. (2008) further demonstrate the fate of these two PLCPs (PIP1 and RCR3) upon inhibition by AVR2. As indicated in their findings, PIP1 and RCR3 are less conserved compared with the other proteases. Sequencing of the region that encodes protease domain of eight wild tomato relatives revealed variant nucleotides in the seven PLCPs, with PIP1 and RCR3 containing variant codons that cause nonsimilar amino acid substitutions. This means that PIP1 and RCR3 are under diversifying selection resulting in variant residues around the substrate binding groove of the protease, deemed to match the coevolving substrates (Shabab et al. 2008). Diversifying selection at interaction surfaces between inhibitors and their substrates are common in plant-pathogen interactions (Misas-Villamil and van der Hoorn 2008). A detailed information was reported by Shabab et al. 2008.
In the study performed by Kaschani et al. (2010), C14 was tagged as a new common target of EPIC1 and EPIC2B, the two apoplastic, cystatin-like proteins secreted by potato typified by a carboxyl-terminal granulin domain. These EPICs proteins strongly inhibit C14, as significant inhibition assays proceed even at lower EPIC concentrations and at wide pH value-range (Kaschani et al. 2010). C14 is a highly conserved cysteine protease in the plant kingdom characterized by a unique, C-terminal granulin-like domain that shares homology with animal growth hormones (Bateman and Bennett 1998). In tomato, C14 was reported to undergo conservative selection which is evident in the resultant few variant codons causing change in the encoded amino acid (Shabab et al. 2008). Possibly, this could be due to the fact that wild tomato is not the natural host of P. infestans (Debener et al. 1990). This prompted the investigators to examine for natural variation of C14 in the natural hosts of P. infestans in the three wild potato species including S. demissum, S. verrucosum, and S. stoliniferum (Debener et al. 1990; Grünwald and Flier 2005; Kaschani et al. 2010). As demonstrated by Kaschani et al. (2010), a total of six new C14 alleles were identified in addition to the C14 allele of cultivated potato deposited in the National Center for Biotechnology Information database. Further, phylogenetic analysis revealed the difference of potato and tomato C14 alleles both at the protein and nucleotide levels marked by expanded branch lengths of potato than in tomato indicating most nucleotide polymorphisms in potato C14s cause amino acid changes (Kaschani et al. 2010). Moreover, as shown using phylogenetic analysis of potato C14 sequences (Yang 2007), there is 95% probability that potato C14 is under diversifying selection due to strong selective pressure from P. infestans. A detailed information was reported by Kaschani et al. 2010.
General inference
Despite the overwhelming number of cysteine proteases in plants, only a handful were successfully investigated. In Arabidopsis alone, functions for only a few of the more than 550 peptidases (Rawlings et al. 2014) have been studied genetically (Adam and Clarke 2002; Beers et al. 2004; van der Hoorn et al. 2004). Introduction of protease activity profiling has been very instrumental in assigning functions for proteases. The current status of cysteine protease research in plant disease immunity features its diverse and overlapping role in basal and effector-triggered immunity. Among the findings, PLCPs are inferred as important target of pathogen effector guarded by other R genes for defense. An intriguing incidence of nucleocytoplasmic trafficking of a few members such as RD19 provides a notion that PCLPs are involved in the crucial transcriptional regulation of defense-related genes in the host. Yet, our understanding regarding how PLCPs shuttle to the nucleus remains obscure. Another exciting inference is the effect of strong selection pressure in inducing variant codons which leads to coevolutionary arms race between R gene and effector under natural pathosystem.
The limited current data insufficiently benchmark our basic understanding on how PLCP activities modulate host-pathogen interaction. Although most of the demonstrated cysteine proteases are closely related, yet they exhibit different roles thus prompting us to explore more PLCP members in many other pathosytems, and extricate further mechanism of defense regulation in the host cell.
This work was supported by the research grant of the Chungbuk National University in 2012, by Golden Seed Project, Ministry of Agriculture, Food and Rural Affairs (MAFRA), and by a grant from the Next-generation BioGreen 21 Program (Project No. PJ008529), Rural Development Administration, Republic of Korea.
Fig. 1
Cellular localization of PLCPs during host-pathogen interaction in six selected pathosystems. Genes in red are host’s PLCP, the green ones are PLCPs secreted by pathogen, the blue ones are non-cysteine protease effectors that interact with host’s PLCP, and the black ones are host’s resistance proteins. (A) Secretion of PopP2 to host’s nucleus recruits RD19 from lytic vacuoles and form nuclear complex which activates RRS1-R mediated resistance (Deslandes and Rivas 2011); (B,C) C. fulvum Avr2 and G. rostochiensis VAP1 target the same protein, Rcr3, in the apoplast to form complex ligand which activates common R protein Cf-2 (Shindo and van der Hoorn 2008); (D) AvrRpt2, a PLCP secreted by P. syringae, inhibits RIN4 and activates RPS2 (Mackey et al. 2003); (E) The Epic2B effector delivered by P. infestans targets and inhibits PIP1, a tomato PLCP secreted in apoplast (Shindo and van der Hoorn 2008); and (F) B. cinerea, a necrotrophic fungus activates RD21, a PLCP localized in vacuole (Shindo et al. 2012).
pbb-02-97f1.jpg
Table 1
Selected papain-like cysteine proteases implicated to play in plant disease and immunity.
Table 1
Protease Source Affinity Function Reference
Cath B N. benthamiana C1A Regulates HR; involved in nonhost and host-specific disease resistance Gilroy et al. 2007
RcR3 S. lycopersicon C1A Common virulence target of C. fulvum Avr2 and G. rostochiensis VAP1 Krüger et al. 2002; Lozano-Torres et al. 2012
PIP1 S. lycopersicon C1A A PR protein and a virulence target of P. infestans EPIC2B Tian et al. 2007
RD19 A. thaliana C1A Virulence target of R. solanacearum PopP2 Bernoux et al. 2008
RD21 A. thaliana C1A Renders protection against necrotrophic pathogen Botrytis cinerea Shindo et al. 2012
PopP1 R. solanacearum C55 Encodes Avr protein which modulates aggressiveness to the host Lavie et al. 2002
AvrPphB P. syringae C58 Inhibits PBS1 kinase and induces HR in Arabidopsis Shao et al. 2003
AvrRpt2 P. syringae C70 Activates RPS2 R-mediated resistance in tomato by inhibiting RIN4 Mackey et al. 2002
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Key Roles of Cysteine Protease in Different Plant Pathosystem
Plant Breed. Biotech.. 2014;2(2):97-109.   Published online June 30, 2014
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Plant Breed. Biotech.. 2014;2(2):97-109.   Published online June 30, 2014
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Key Roles of Cysteine Protease in Different Plant Pathosystem
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Fig. 1 Cellular localization of PLCPs during host-pathogen interaction in six selected pathosystems. Genes in red are host’s PLCP, the green ones are PLCPs secreted by pathogen, the blue ones are non-cysteine protease effectors that interact with host’s PLCP, and the black ones are host’s resistance proteins. (A) Secretion of PopP2 to host’s nucleus recruits RD19 from lytic vacuoles and form nuclear complex which activates RRS1-R mediated resistance (Deslandes and Rivas 2011); (B,C) C. fulvum Avr2 and G. rostochiensis VAP1 target the same protein, Rcr3, in the apoplast to form complex ligand which activates common R protein Cf-2 (Shindo and van der Hoorn 2008); (D) AvrRpt2, a PLCP secreted by P. syringae, inhibits RIN4 and activates RPS2 (Mackey et al. 2003); (E) The Epic2B effector delivered by P. infestans targets and inhibits PIP1, a tomato PLCP secreted in apoplast (Shindo and van der Hoorn 2008); and (F) B. cinerea, a necrotrophic fungus activates RD21, a PLCP localized in vacuole (Shindo et al. 2012).
Key Roles of Cysteine Protease in Different Plant Pathosystem

Selected papain-like cysteine proteases implicated to play in plant disease and immunity.

Protease Source Affinity Function Reference
Cath B N. benthamiana C1A Regulates HR; involved in nonhost and host-specific disease resistance Gilroy et al. 2007
RcR3 S. lycopersicon C1A Common virulence target of C. fulvum Avr2 and G. rostochiensis VAP1 Krüger et al. 2002; Lozano-Torres et al. 2012
PIP1 S. lycopersicon C1A A PR protein and a virulence target of P. infestans EPIC2B Tian et al. 2007
RD19 A. thaliana C1A Virulence target of R. solanacearum PopP2 Bernoux et al. 2008
RD21 A. thaliana C1A Renders protection against necrotrophic pathogen Botrytis cinerea Shindo et al. 2012
PopP1 R. solanacearum C55 Encodes Avr protein which modulates aggressiveness to the host Lavie et al. 2002
AvrPphB P. syringae C58 Inhibits PBS1 kinase and induces HR in Arabidopsis Shao et al. 2003
AvrRpt2 P. syringae C70 Activates RPS2 R-mediated resistance in tomato by inhibiting RIN4 Mackey et al. 2002
Table 1 Selected papain-like cysteine proteases implicated to play in plant disease and immunity.