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Volume 5(1); March 2017

Research Articles
Overexpression of AtSZF2 from Arabidopsis Showed Enhanced Tolerance to Salt Stress in Soybean
Mi-Jin Kim, Hye Jeong Kim, Jung Hun Pak, Hyun Suk Cho, Hong Kyu Choi, Ho Won Jung, Dong Hee Lee, Young-Soo Chung
Plant Breed. Biotech. 2017;5(1):1-15.   Published online March 1, 2017
DOI: https://doi.org/10.9787/PBB.2017.5.1.1

Plants have adapted to environmental challenges by expressing many plant genes in response to the stresses. Among those genes, CCCH zinc finger proteins are involved in abiotic and biotic stresses. Transgenic soybean plants overexpressing AtSZF2 were produced to investigate that its ectopic overexpression enhanced salt stress tolerance by Agrobacterium-mediated transformation using half-seed explants. Sixteen transgenic lines were chosen to analyze for T-DNA insertion and transcription levels, and most of them were confirmed as positive. In further analysis with Southern blot, stable transformation event and copy number were confirmed. Following high salinity stress on the detached leaf and whole plant of two transgenic lines (#4 and #6) revealed that the ectopic expression of AtSZF2 was correlated with stress tolerance in phenotype, ion leakage and chlorophyll content with statistical significance. In another test with 20% PEG treatment, similar tolerance of transgenic plants was observed with lower ion leakage and higher chlorophyll content, indicating that the damage of cell membrane was prevented in transgenic plants. Finally, expression of various abiotic stress-responding genes was detected by reverse transcriptase and quantitative real-time PCR analysis with the transgenic plants. It could be proposed that introduction of AtSZF2 resulted in the modulation of ABA/stress responsive gene expression in transgenic soybean plants and make them tolerant against salt stress. Considering soybean as a salt-sensitive crop and importance of salt stress tolerance in specific farming region, the introduction of AtSZF2 may provide an approach for crop improvement in soybean breeding.

Citations

Citations to this article as recorded by  
  • Resilient soybeans for a changing climate: analyzing traditional and emerging new plant breeding technologies to combat abiotic stresses
    Bareera Nasir, Saleem Ur Rahman, Abdaal Ali, Ehtisham Shafique, Nighat Zia, Niaz Ahmad, Ghulam Raza, Rubina Bukhari
    Acta Physiologiae Plantarum.2025;[Epub]     CrossRef
  • CRISPR/Cas9-mediated simultaneous targeting of GmP34 and its homologs produces T-DNA-free soybean mutants with reduced allergenic potential
    Dongwon Baek, Byung Jun Jin, Mi Suk Park, Ye Jin Cha, Tae Hee Han, Ye Na Jang, Su Bin Kim, Sang In Shim, Jong-Il Chung, Hyun Jin Chun, Min Chul Kim
    Frontiers in Plant Science.2025;[Epub]     CrossRef
  • Soybean Molecular Breeding Through Genome Editing Tools: Recent Advances and Future Perspectives
    Chan Yong Kim, Sivabalan Karthik, Hyeran Kim
    Agronomy.2025; 15(8): 1983.     CrossRef
  • Influence of arbuscular mycorrhizal fungi on morpho-biochemical characteristics, nutrient uptake, and transcriptomic profile of Solanum melongena L. plant
    Subhesh Saurabh Jha, L. S. Songachan
    3 Biotech.2025;[Epub]     CrossRef
  • A novel PGPR strain, Streptomyces lasalocidi JCM 3373T, alleviates salt stress and shapes root architecture in soybean by secreting indole‐3‐carboxaldehyde
    Liang Lu, Ning Liu, Zihui Fan, Minghao Liu, Xiaxia Zhang, Juan Tian, Yanjun Yu, Honghui Lin, Ying Huang, Zhaosheng Kong
    Plant, Cell & Environment.2024; 47(6): 1941.     CrossRef
  • RL-WG26 mediated salt stress tolerance in rice seedlings: A new insight into molecular mechanisms
    Lei Ren, Yi Zhang, John L. Zhou, Guan Wang, Yujian Mo, Yu Ling, Yongxiang Huang, Yueqing Zhang, Hanqiao Hu, Yanyan Wang
    Plant Stress.2024; 11: 100306.     CrossRef
  • Halotolerant endophytes promote grapevine regrowth after salt-induced defoliation
    Salvadora Navarro-Torre, Sara Ferrario, Ana D. Caperta, Gonçalo Victorino, Marion Bailly, Vicelina Sousa, Wanda Viegas, Amaia Nogales
    Journal of Plant Interactions.2023;[Epub]     CrossRef
  • Environmental Risk Assessment of Herbicide Resistant Transgenic Rapeseed (Brassica napus L.) : Responses to Cyprinus carpio fed on herbicide resistant transgenic rapeseed
    Sung-Dug Oh, Kyunglyung Baek, Seok-Ki Min, Joon Ki Hong, Doh-Won Yun, Seong-Kon Lee, Ancheol Chang
    Journal of the Korean Society of International Agriculture.2023; 35(4): 278.     CrossRef
  • Mutation of GmIPK1 Gene Using CRISPR/Cas9 Reduced Phytic Acid Content in Soybean Seeds
    Ji Hyeon Song, Gilok Shin, Hye Jeong Kim, Saet Buyl Lee, Ju Yeon Moon, Jae Cheol Jeong, Hong-Kyu Choi, In Ah Kim, Hyeon Jin Song, Cha Young Kim, Young-Soo Chung
    International Journal of Molecular Sciences.2022; 23(18): 10583.     CrossRef
  • A Review of Recent Advances and Future Directions in the Management of Salinity Stress in Finger Millet
    Wilton Mbinda, Asunta Mukami
    Frontiers in Plant Science.2021;[Epub]     CrossRef
  • Overexpression of Arabidopsis thaliana blue-light inhibitor of cryptochromes 1 gene alters plant architecture in soybean
    Hyun Suk Cho, Yoon Jeong Lee, Hye Jeong Kim, Moon-Young Park, Wan Woo Yeom, Ji Hyeon Song, In Ah Kim, Seong-Hyeon Kim, Jeong-Il Kim, Young-Soo Chung
    Plant Biotechnology Reports.2021; 15(4): 459.     CrossRef
  • Improved salt tolerance of Chenopodium quinoa Willd. contributed by Pseudomonas sp. strain M30-35
    Deyu Cai, Ying Xu, Fei Zhao, Yan Zhang, Huirong Duan, Xiaonong Guo
    PeerJ.2021; 9: e10702.     CrossRef
  • Morphological, physiological, and biochemical responses of Tunisian Urtica pilulifera L. under salt constraint
    Ghazouani Soumaya, Hannachi Hédia, Ben Nasri- Ayachi Mouhiba
    South African Journal of Botany.2021; 142: 124.     CrossRef
  • Serratia marcescens BM1 Enhances Cadmium Stress Tolerance and Phytoremediation Potential of Soybean Through Modulation of Osmolytes, Leaf Gas Exchange, Antioxidant Machinery, and Stress-Responsive Genes Expression
    Mohamed A. El-Esawi, Amr Elkelish, Mona Soliman, Hosam O. Elansary, Abbu Zaid, Shabir H. Wani
    Antioxidants.2020; 9(1): 43.     CrossRef
  • Overexpression of AtYUCCA6 in soybean crop results in reduced ROS production and increased drought tolerance
    Jin Sol Park, Hye Jeong Kim, Hyun Suk Cho, Ho Won Jung, Joon-Young Cha, Dae-Jin Yun, Seon-Woo Oh, Young-Soo Chung
    Plant Biotechnology Reports.2019; 13(2): 161.     CrossRef
  • Co‐expression of Arabidopsis AtAVP1 and AtNHX1 to Improve Salt Tolerance in Soybean
    Nga T. Nguyen, Hop T. Vu, Trang T. Nguyen, Lan-Anh T. Nguyen, Minh-Chanh D. Nguyen, Khang L. Hoang, Khanh T. Nguyen, Truyen N. Quach
    Crop Science.2019; 59(3): 1133.     CrossRef
  • Salinity stress response and ‘omics’ approaches for improving salinity stress tolerance in major grain legumes
    Uday Chand Jha, Abhishek Bohra, Rintu Jha, Swarup Kumar Parida
    Plant Cell Reports.2019; 38(3): 255.     CrossRef
  • Serratia liquefaciens KM4 Improves Salt Stress Tolerance in Maize by Regulating Redox Potential, Ion Homeostasis, Leaf Gas Exchange and Stress-Related Gene Expression
    Mohamed A. El-Esawi, Ibrahim A. Alaraidh, Abdulaziz A. Alsahli, Saud M. Alzahrani, Hayssam M. Ali, Aisha A. Alayafi, Margaret Ahmad
    International Journal of Molecular Sciences.2018; 19(11): 3310.     CrossRef
  • Bacillus firmus (SW5) augments salt tolerance in soybean (Glycine max L.) by modulating root system architecture, antioxidant defense systems and stress-responsive genes expression
    Mohamed A. El-Esawi, Ibrahim A. Alaraidh, Abdulaziz A. Alsahli, Saud A. Alamri, Hayssam M. Ali, Aisha A. Alayafi
    Plant Physiology and Biochemistry.2018; 132: 375.     CrossRef
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Authentication of Golden-Berry P. ginseng Cultivar ‘Gumpoong’ from a Landrace ‘Hwangsook’ Based on Pooling Method Using Chloroplast-Derived Markers
Ho Jun Joh, Nam-Hoon Kim, Murukarthick Jayakodi, Woojong Jang, Jee Young Park, Young Chang Kim, Jun-Gyo In, Tae-Jin Yang
Plant Breed. Biotech. 2017;5(1):16-24.   Published online March 1, 2017
DOI: https://doi.org/10.9787/PBB.2017.5.1.16

Most ginseng cultivars bear red berry and only one cultivar ‘Gumpoong’ (GU) bears golden berry. GU is an elite cultivar bred by pedigree selection from a golden berry landrace (a mixed population) ‘Hwangsook’ (HS). We developed three unique polymorphic markers from complete chloroplast genome sequences of GU and HS. A population of GU showed uniform band amplicon against three chloroplast markers whereas HS population displayed mixed genotypes for both GU and HS. Using the characteristics of mixed genotypes in HS population, we developed a convenient method to differentiate GU and HS population by application of pooled DNA template for PCR analysis (pooling method). The pooling method revealed that the GU pool was identical with GU genotype while the HS pool showed both GU and HS genotype. The pooling method is a cost and time effective method for accurate authentication of both golden berry ginseng cultivars. The method is useful to protect GU products from its tentative counterfeits from seeds to mature plant stages as well as processed root products.

Citations

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    Chunhui Zhao, Xinyi Li, Xiu Lan, Rupeng Zhao, Ruolan Huang, Lixia Ruan, Zhaoqin Cai, Zhenling Huang, Wanling Wei, Huixian Chen, Hengrui Li, Haixia Yang
    BMC Genomics.2025;[Epub]     CrossRef
  • Molecular authentication of Paeonia species for paeonia radix production using plastid and nuclear DNA markers
    Jiseok Kim, Jong-Soo Kang, Hyun-Seung Park, Jae-Hyeon Jeon, Jee Young Park, Eunbi Yeo, Jung Hwa Kang, Seung Hyun Kim, Do Won Jeong, Young-Sik Kim, Hocheol Kim, Woojong Jang, Goya Choi, Byeong Cheol Moon, Tae-Jin Yang
    Journal of Applied Research on Medicinal and Aromatic Plants.2025; 44: 100604.     CrossRef
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    Sangjin Go, Hyunjin Koo, Minah Jung, Seongmin Hong, Gibum Yi, Yong-Min Kim
    Scientific Data.2024;[Epub]     CrossRef
  • High-throughput discovery of plastid genes causing albino phenotypes in ornamental chimeric plants
    Hyun-Seung Park, Jae-Hyeon Jeon, Woohyeon Cho, Yeonjeong Lee, Jee Young Park, Jiseok Kim, Young Sang Park, Hyun Jo Koo, Jung Hwa Kang, Taek Joo Lee, Sang Hoon Kim, Jin-Baek Kim, Hae-Yun Kwon, Suk-Hwan Kim, Nam-Chon Paek, Geupil Jang, Jeong-Yong Suh, Tae-J
    Horticulture Research.2023;[Epub]     CrossRef
  • High-Throughput Digital Genotyping Tools for Panax ginseng Based on Diversity among 44 Complete Plastid Genomes
    Woojong Jang, Yeeun Jang, Woohyeon Cho, Sae Hyun Lee, Hyeonah Shim, Jee Young Park, Jiang Xu, Xiaofeng Shen, Baosheng Liao, Ick-Hyun Jo, Young Chang Kim, Tae-Jin Yang
    Plant Breeding and Biotechnology.2022; 10(3): 174.     CrossRef
  • Complete plastid and 45S rDNA sequences allow authentication of Liriope platyphylla and Ophiopogon japonicus
    Yeonjeong Lee, Hyun-Seung Park, Jae-Hyeon Jeon, Jee Young Park, Seung Hyun Kim, Jungmoo Huh, Sunmin Woo, Do-Won Jeong, Tae-Jin Yang
    Current Plant Biology.2022; 30: 100244.     CrossRef
  • Nuclear and chloroplast genome diversity revealed by low-coverage whole-genome shotgun sequence in 44 Brassica oleracea breeding lines
    Sampath Perumal, Nomar Espinosa Waminal, Jonghoon Lee, Hyun-Jin Koo, Boem-soon Choi, Jee Young Park, Kyounggu Ahn, Tae-Jin Yang
    Horticultural Plant Journal.2021; 7(6): 539.     CrossRef
  • Inheritance of chloroplast and mitochondrial genomes in cucumber revealed by four reciprocal F1 hybrid combinations
    Hyun-Seung Park, Won Kyung Lee, Sang-Choon Lee, Hyun Oh Lee, Ho Jun Joh, Jee Young Park, Sunggil Kim, Kihwan Song, Tae-Jin Yang
    Scientific Reports.2021;[Epub]     CrossRef
  • The complete chloroplast genome of the Lonicera maackii (Caprifoliaceae), an ornamental plant
    Guolun Jia, Huan Wang, Pei Yu, Peng Li
    Mitochondrial DNA Part B.2020; 5(1): 560.     CrossRef
  • Genetic diversity among cultivated and wild Panax ginseng populations revealed by high-resolution microsatellite markers
    Woojong Jang, Yeeun Jang, Nam-Hoon Kim, Nomar Espinosa Waminal, Young Chang Kim, Jung Woo Lee, Tae-Jin Yang
    Journal of Ginseng Research.2020; 44(4): 637.     CrossRef
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    Hyun-Seung Park, Murukarthick Jayakodi, Sae Hyun Lee, Jae-Hyeon Jeon, Hyun-Oh Lee, Jee Young Park, Byeong Cheol Moon, Chang-Kug Kim, Rod A. Wing, Steven G. Newmaster, Ji Yeon Kim, Tae-Jin Yang
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    Hao Zhang, Suleman Abid, Jong Chan Ahn, Ramya Mathiyalagan, Yu-Jin Kim, Deok-Chun Yang, Yingping Wang
    Molecules.2020; 25(11): 2635.     CrossRef
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    Hyun Oh Lee, Ho Jun Joh, Kyunghee Kim, Sang-Choon Lee, Nam-Hoon Kim, Jee Young Park, Hyun-Seung Park, Mi-So Park, Soonok Kim, Myounghai Kwak, Kyu-yeob Kim, Woo Kyu Lee, Tae-Jin Yang
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    Shin-Jae Kang, Jee Young Park, Woojong Jang, Hyun Jo Koo, Dong Young Lee, Mi Song Kim, Sang Il Han, Sang Hyun Sung, Tae-Jin Yang
    Mitochondrial DNA Part B.2019; 4(1): 1008.     CrossRef
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    Plant Biotechnology Reports.2019; 13(1): 51.     CrossRef
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    Jae-Hyeon Jeon, Hyun-Seung Park, Jee Young Park, Tae Sun Kang, Kisung Kwon, Yeon Bok Kim, Jong-Won Han, Seung Hyun Kim, Sang Hyun Sung, Tae-Jin Yang
    Mitochondrial DNA Part B.2019; 4(1): 176.     CrossRef
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    Mitochondrial DNA Part B.2018; 3(2): 941.     CrossRef
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    Seung Woo Jin, Jee Young Park, Shin-Jae Kang, Hyun-Seung Park, Hyeonah Shim, Taek Joo Lee, Jung Hwa Kang, Sang Hyun Sung, Tae-Jin Yang
    Mitochondrial DNA Part B.2018; 3(2): 1210.     CrossRef
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A Glimpse of Panax ginseng Genome Structure Revealed from Ten BAC Clone Sequences Obtained by SMRT Sequencing Platform
Woojong Jang, Nam-Hoon Kim, Junki Lee, Nomar Espinosa Waminal, Sang-Choon Lee, Murukarthick Jayakodi, Hong-Il Choi, Jee Young Park, Jong-Eun Lee, Tae-Jin Yang
Plant Breed. Biotech. 2017;5(1):25-35.   Published online March 1, 2017
DOI: https://doi.org/10.9787/PBB.2017.5.1.25

Korean ginseng (Panax ginseng) is a well-known valuable medicinal plant with excellent therapeutic effects, however its complex genome structure has not been elucidated yet. To understand its genome structure, we obtained ten ginseng bacterial artificial chromosome (BAC) clone sequences by single-molecule real-time (SMRT) sequencing platform using a pooled DNA of the BAC clones. Out of the ten BAC clones, nine were completely assembled without any gap and one remained a single gap. The total length of BAC clone sequences was 1,163,364 bp. Sophisticated sequence analysis revealed that the 89.7% of the sequences are high copy repeat regions and the remaining 10.3% are non-repeat regions. Eleven protein-coding genes were identified in the non-repeat regions. Most of the repeat regions show more than 1,000 copies and complex structure of various repetitive elements. Ty3/Gypsy family long terminal repeat retrotransposons (LTR-RTs) are predominant repeats occupying 46.9% of the 1,163-kbp sequence. We identified six novel LTR-RTs and their insertion time. Fluorescence in situ hybridization (FISH) analysis demonstrated that PgDel2 and PgDel5 elements had a subgenome-biased distribution. Collectively, our analysis reveals that ginseng genome has very complex genome structure with abundant repetitive elements and rare gene frequency.

Citations

Citations to this article as recorded by  
  • High-resolution genetic map and SNP chip for molecular breeding in Panax ginseng, a tetraploid medicinal plant
    Woohyeon Cho, Woojong Jang, Hyeonah Shim, Jiseok Kim, Youngju Oh, Jee Young Park, Young Chang Kim, Jung-Woo Lee, Ick-Hyun Jo, Misun Lee, Jinsu Gil, Martin Mascher, Murukarthick Jayakodi, Xuejiao Liao, Jiang Xu, Deqiang Dou, Yi Lee, Tae-Jin Yang
    Horticulture Research.2024;[Epub]     CrossRef
  • Beyond genome: Advanced omics progress of Panax ginseng
    Wenjing Yu, Siyuan Cai, Jiali Zhao, Shuhan Hu, Chen Zang, Jiang Xu, Lianghai Hu
    Plant Science.2024; 341: 112022.     CrossRef
  • Cytokinin signaling promotes root secondary growth and bud formation in Panax ginseng
    Kyoung Rok Geem, Yookyung Lim, Jeongeui Hong, Wonsil Bae, Jinsu Lee, Soeun Han, Jinsu Gil, Hyunwoo Cho, Hojin Ryu
    Journal of Ginseng Research.2024; 48(2): 220.     CrossRef
  • Construction of a Single File Reference Transcriptome Database for Deodeok (Codonopsis lanceolata) and Sseumbagwi (Ixeridium dentata)
    Tae-Ho Lee, Yun-Ho Oh, Ji-Nam Kang, Si-Myung Lee
    Korean Journal of Breeding Science.2023; 55(4): 321.     CrossRef
  • Salinity responses and tolerance mechanisms in underground vegetable crops: an integrative review
    Kumar Nishant Chourasia, Sanket Jijabrao More, Ashok Kumar, Dharmendra Kumar, Brajesh Singh, Vinay Bhardwaj, Awadhesh Kumar, Sourav Kumar Das, Rajesh Kumar Singh, Gaurav Zinta, Rahul Kumar Tiwari, Milan Kumar Lal
    Planta.2022;[Epub]     CrossRef
  • Dynamic evolution of Panax species
    Hyeonah Shim, Nomar Espinosa Waminal, Hyun Hee Kim, Tae-Jin Yang
    Genes & Genomics.2021; 43(3): 209.     CrossRef
  • Gibberellin Signaling Promotes the Secondary Growth of Storage Roots in Panax ginseng
    Chang Pyo Hong, Jinsoo Kim, Jinsu Lee, Seung-il Yoo, Wonsil Bae, Kyoung Rok Geem, Jin Yu, Inbae Jang, Ick Hyun Jo, Hyunwoo Cho, Donghwan Shim, Hojin Ryu
    International Journal of Molecular Sciences.2021; 22(16): 8694.     CrossRef
  • Genetic diversity among cultivated and wild Panax ginseng populations revealed by high-resolution microsatellite markers
    Woojong Jang, Yeeun Jang, Nam-Hoon Kim, Nomar Espinosa Waminal, Young Chang Kim, Jung Woo Lee, Tae-Jin Yang
    Journal of Ginseng Research.2020; 44(4): 637.     CrossRef
  • Till 2018: a survey of biomolecular sequences in genus Panax
    Vinothini Boopathi, Sathiyamoorthy Subramaniyam, Ramya Mathiyalagan, Deok-Chun Yang
    Journal of Ginseng Research.2020; 44(1): 33.     CrossRef
  • Five-color fluorescence in situ hybridization system for karyotyping of Panax ginseng
    Nomar Espinosa Waminal, Tae-Jin Yang, Jun-Gyo In, Hyun Hee Kim
    Horticulture, Environment, and Biotechnology.2020; 61(5): 869.     CrossRef
  • Complete Mitochondrial Genome and a Set of 10 Novel Kompetitive Allele-Specific PCR Markers in Ginseng (Panax ginseng C. A. Mey.)
    Woojong Jang, Hyun Oh Lee, Jang-Uk Kim, Jung-Woo Lee, Chi-Eun Hong, Kyong-Hwan Bang, Jong-Wook Chung, Ick-Hyun Jo
    Agronomy.2020; 10(12): 1868.     CrossRef
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    Agronomy.2020; 10(1): 68.     CrossRef
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    Nam‐Hoon Kim, Murukarthick Jayakodi, Sang‐Choon Lee, Beom‐Soon Choi, Woojong Jang, Junki Lee, Hyun Hee Kim, Nomar E. Waminal, Meiyappan Lakshmanan, Binh van Nguyen, Yun Sun Lee, Hyun‐Seung Park, Hyun Jo Koo, Jee Young Park, Sampath Perumal, Ho Jun Joh, Ha
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    Ick-Hyun Jo, Jinsu Lee, Chi Hong, Dong Lee, Wonsil Bae, Sin-Gi Park, Yong Ahn, Young Kim, Jang Kim, Jung Lee, Dong Hyun, Sung-Keun Rhee, Chang Hong, Kyong Bang, Hojin Ryu
    Genes.2017; 8(9): 228.     CrossRef
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Genetic and Environmental Variation of First Pod Height in Soybean [Glycine max (L.) Merr.]
Beom-Kyu Kang, Hyun-Tae Kim, Man-Soo Choi, Seong-Chul Koo, Jeong-Hyun Seo, Hong-Sik Kim, Sang-Ouk Shin, Hong-Tae Yun, In-Seok Oh, Krishnanand P. Kulkarni, Jeong-Dong Lee
Plant Breed. Biotech. 2017;5(1):36-44.   Published online March 1, 2017
DOI: https://doi.org/10.9787/PBB.2017.5.1.36

First pod height (FPH) is an agronomic trait for the mechanical harvesting of soybeans with combines. The seed loss could be minimized, if the FPH is higher than the height of the cutter bar in combines. Hence, developing soybeans with high FPH has become one of important breeding goals in current crop improvement programs. The
objective
of this study was to evaluate genetic and environmental variation of FPH in soybean and to analyze the effect of ratio of FPH to plant height (PH) on seed yield. Four genotypes were evaluated across six different environments to analyze environmental variation of agronomic traits including FPH. Three F2 populations were evaluated to analyze genetic variation and relationship between the ratio of FPH to PH and seed yield. The main effects of planting distance, genotype and seeding date were significant for FPH, but FPH is affected more by genetic factors than by environmental factors. The mean heritability value of FPH was 66% across three F2 populations. Seed yield was found to reduce with increase in the FPH/PH ratio. In conclusion, genetic factors have effect more than environments to the variation of FPH. While FPH is higher than cutting height, the smaller ratio can minimize seed yield decrease.

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    Jariya Chinnarat, Tidarat Monkham, Jirawat Sanitchon, Sompong Chankaew
    Agronomy.2025; 15(3): 600.     CrossRef
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    Ankita Thapar, Pham Anh Tuan, Amarjot Kaur, Deepak Sharma, Belay T. Ayele
    Journal of Plant Growth Regulation.2025; 44(9): 5575.     CrossRef
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    Namgeol Kim, Seuk-Ki Lee, Yo-han Yoo, Inhye Lee, Kwang-soo Cho, Min-Jung Seo, BeomKyu Kang, JeongHyun Seo, JunHoi Kim, SuVin Heo, Jinsil Choi, Hyeon Tae Cho
    Korean Journal of Breeding Science.2025; 57(3): 315.     CrossRef
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    Małgorzata Gniadzik-Zasańska, Marcin Kozak, Anna Wondołowska-Grabowska
    Agronomy Science.2024; 79(1): 41.     CrossRef
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    Genís Simon-Miquel, Moritz Reckling, Daniel Plaza-Bonilla
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    Beom Kyu Kang, Jeong Hyun Seo, Jun Hoi Kim, Su Vin Heo, Gi Rim Park, Won Young Han, Myung Chul Seo, Yeong Hoon Lee, In Youl Baek, Jee Yeon Ko, Ji Hee Park, Jung Suk Sung, Hong Sik Kim, Chan Sik Jung, Hye Sun Choi, Yeong Min Jo, Eun Byul Go, Ji Ae Lee
    Korean Journal of Breeding Science.2024; 56(4): 547.     CrossRef
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Identification of a Novel DFR-A Mutant Allele Determining the Bulb Color Difference between Red and Yellow Onions (Allium cepa L.)
Bongju Kim, Youngcho Cho, Sunggil Kim
Plant Breed. Biotech. 2017;5(1):45-53.   Published online March 1, 2017
DOI: https://doi.org/10.9787/PBB.2017.5.1.45

To introduce downy mildew resistance from a yellow-colored resistant cultivar, ‘Santero’, into a yellow breeding line, OT803, the F1 hybrid was produced by crossing Santero and OT803. The bulb color of the F1 hybrids became light pink, suggesting involvement of complementation between the DFR-A and ANS genes in the onion anthocyanin biosynthesis pathway. Since Santero contained active DFR-A and inactive ANS alleles, OT803 was assumed to harbor active ANS and inactive DFR-A alleles. However, some yellow-colored individuals of OT803 were shown to contain the homozygous genotype of the active DFR-AR4-like allele. Sequencing of 4,830-bp full-length sequences of this DFR-AR4-like allele revealed that the nucleotide sequences of the DFR-AR4 and DFR-AR4-like alleles were identical except for a single nucleotide deletion in the last exon. This single base-pair deletion resulted in creation of a premature stop codon at 2-bp downstream of the deletion mutation. This new DFR-A mutant allele was designated DFR-APS2. The RT-PCR results showed that transcripts of the DFR-APS2 allele were significantly reduced, suggesting involvement of nonsense-mediated mRNA decay (NMD) mechanism. The systematic process consisting of PCR amplification and sequencing of the PCR products was modified to identify the DFR-APS2 allele among 16 different DFR-A alleles. No additional accession was found to contain the DFR-APS2 allele from 155 diverse onion germplasm, indicating very limited distribution of this new DFR-APS2 allele.

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