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Volume 7(1); March 2019

Research Articles

Identifying a Candidate Mutation Underlying a Reduced Cuticle Wax Mutant of Rice Using Targeted Exon Capture and Sequencing
HyunJung Kim, Thomas H. Tai
Plant Breed. Biotech. 2019;7(1):1-11.   Published online March 1, 2019
DOI: https://doi.org/10.9787/PBB.2019.7.1.1

Aerial surfaces of terrestrial plants are protected from the uncontrolled loss of water and gas by the cuticle, a membrane of fatty acid polymers on the outer surface of epidermal cells. Composed of cutin and waxes, the cuticle protects against a wide range of external stresses and has an important role in plant development and reproduction. Plants with reduced cuticular waxes often exhibit glossy, bright green leaves, which in rice are only observed in the presence of water adhesion. In this study, a wet leaf/glossy (wlg) mutant KDS-2249D was subjected to targeted exon capture and sequencing to identify candidate mutations. A single nonsynonymous, homozygous mutation was found in the KDS-2249D mutant. The mutation (G1080A) is predicted to change a tryptophan at position 360 to a stop codon in the Glossy1-like-1/wax crystal-sparse leaf 2 gene. This mutation completely co-segregated with the wlg phenotype in an F2 mapping population (n = 435) and the KDS-2249D mutant exhibited a 40–50% decrease in total wax and significant increase in membrane permeability. This mutant will be useful for studies examining the role of cuticle waxes in protecting rice plants from environmental stresses.

Citations

Citations to this article as recorded by  
  • Identification of a novel mutant allele of the LIKE EARLY STARVATION 1 gene in rice (Oryza sativa L.) using QTL-seq
    Kyu-Chan Shim, HyunJung Kim, Mi-Ra Yoon, Sung-Ho Na, Sa-Eun Park, Sang-Nag Ahn, Thomas H Tai, J Holland
    G3: Genes, Genomes, Genetics.2026;[Epub]     CrossRef
  • Comparative Genomic Analysis and Functional Identification of CER1 and CER3 Homologs in Rice Wax Synthesis
    Nesma E. E. Youssif, Bowen Yang, Haodong Huang, Mohamed Hamdy Amar, Mohamed Ezzat, Mohammad Belal, Sanaa A. M. Zaghlool, Huayan Zhao, Dong Fu, Shiyou Lü
    Biology.2026; 15(2): 166.     CrossRef
  • Identification and bioinformatic characterization of rare variants of Rhododendron canescens architecture genes
    Lav K. Yadav, H. Dayton Wilde
    Euphytica.2022;[Epub]     CrossRef
  • Identification of Rice Mutants with Altered Grain Alkali Digestion Trait
    HyunJung Kim, Ralph Vin B. Imatong, Thomas H. Tai
    Plant Breeding and Biotechnology.2020; 8(1): 19.     CrossRef
  • Deciphering the Novel Role of AtMIN7 in Cuticle Formation and Defense against the Bacterial Pathogen Infection
    Zhenzhen Zhao, Xianpeng Yang, Shiyou Lü, Jiangbo Fan, Stephen Opiyo, Piao Yang, Jack Mangold, David Mackey, Ye Xia
    International Journal of Molecular Sciences.2020; 21(15): 5547.     CrossRef
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Evaluation of Advanced Breeding Lines of Cowpea (Vigna unguiculata L. Walp) for High Seed Yield under Farmers’ Field Conditions
Olawale Mashood Aliyu, Oluwafemi Oluwatosin Lawal, Abdulkabir Adesina Wahab, Usman Yaman Ibrahim
Plant Breed. Biotech. 2019;7(1):12-23.   Published online March 1, 2019
DOI: https://doi.org/10.9787/PBB.2019.7.1.12

Climate change has imposed greater challenge on cowpea production in the savannah ecology of West Africa sub-region in the recent time, however, development of varieties that combined resilience (stability) and precocity with high seed yield would be a sustainable approach to mitigate this problem. To this end, nine advanced breeding lines were evaluated along with two commercial varieties across three locations in guinea savannah ecology, using a randomized complete block design of three replications. Results obtained for seed yield and yield components indicate that the eleven cowpeas exhibited substantial variability for all plant traits studied and implications discussed. Additive Main Effect and Multiplicative Interaction (AMMI) analysis however revealed that the variations recorded were substantially attributable to genotypic component (70–80%) and less of environment (0.7–7.0%), a measure of phenotypic stability of these cowpea lines. However, seed yield and yield components vary significantly across the three locations, which further emphasize the important role of soil and climatic variables to cowpea production. In this study, two varieties (IT07K-299-6 and IT11K-61-82) consistently combined high seed yield (> 2 tons/ha) with precocity across the three locations, and could be multiplied for distribution to farmers as short-term intervention for yield increase. Reduced seed viability of these varieties reflects seed storage challenge in cowpea farming. In addition to significant contributions of some yield components to seed yield, there was evidence of strong association between precocity and high yield, and its implication for cowpea improvement discussed.

Citations

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  • The Yield and Pest Trade-Off: An Analysis of Insecticide Spray Frequency on the Performance of a Genetically Modified Cowpea in Ghana
    Jerry A. Nboyine, Gloria A. Adazebra, Philip Agrengsore, Ebenezer Asamani, Mukhtaru Zakaria, James Y. Kwabena, Haruna K. Ali, Theophilus K. Tengey, Prince M. Etwire, Jose M. Barrero
    Agronomy.2026; 16(2): 156.     CrossRef
  • Genotypic Variation in Phosphorus-Use Efficiency Underpins Yield Stability and Drives Soil Regeneration in Cowpea (Vigna unguiculata L. Walp.)
    Olawale Mashood Aliyu, Kafayat Temitope Oladimeji, Yusuf Folorunsho Abdulkareem, Toyeeb Alabi, Tajudeen Afimoh Olajide, Wasilat Mojirola Muhammed
    Journal of Soil Science and Plant Nutrition.2026;[Epub]     CrossRef
  • Agronomic evaluation of cowpea (Vigna unguiculata L.) lines for varietal development in the Brekum West municipality of Ghana
    John Abugri Amenga, Esther Fobi Donkor, Kwabena Atakora, Samuel Novor
    Ecological Genetics and Genomics.2025; 34: 100332.     CrossRef
  • Evaluation of Cowpea Landraces under a Mediterranean Climate
    Efstathia Lazaridi, Penelope J. Bebeli
    Plants.2023; 12(10): 1947.     CrossRef
  • Cowpea Constraints and Breeding in Europe
    Efstathia Lazaridi, Penelope J. Bebeli
    Plants.2023; 12(6): 1339.     CrossRef
  • Understanding the Nexus of Genotype, Root Nodulation, and Soil Nutrients for Shoot Biomass Production and Seed Yield in Cowpea (Vigna unguiculata L. Walp)
    Olawale Mashood Aliyu, Toyeeb Abayomi Abioye, Yusuf Folorunsho Abdulkareem, Abdulqudus Ibrahim
    Journal of Soil Science and Plant Nutrition.2023; 23(2): 2566.     CrossRef
  • Genotype x environment interaction and stability in landraces of cowpea under dryland conditions
    Rubens R. Rolim, Naysa F. F. do Nascimento, Mayana F. Nascimento, Helder F. P. de Araujo
    Revista Caatinga.2023; 36(2): 339.     CrossRef
  • Genotype × environment interaction and adaptation of cowpea genotypes across six planting seasons
    Abiola AJAYİ, Alaba GBADAMOSİ, Oluwatoyin OSEKİTA, Babatunde TAİWO, Ato Babawole FAWIBE, Iyanu ADEDEJİ, Temitope OMİSAKİN
    Frontiers in Life Sciences and Related Technologies.2022; 3(1): 7.     CrossRef
  • Influence of air humidity on variability of morphological features of Vigna unguiculata (L.) Walp. in artificial conditions
    Ekaterina A. Krylova, Elena K. Khlestkina, Marina O. Burlyaeva
    Ecological genetics.2022; 20(3): 215.     CrossRef
  • Assessment of growth and development of cowpea varieties based on phenological and morphological observations
    Iryna Bobos, Oleksandr Komar, Ivan Fedosiy
    PLANT AND SOIL SCIENCE.2022;[Epub]     CrossRef
  • Variance components, correlation and path analyses in cowpea (Vigna unguiculata L., Walp)
    Olawale Mashood Aliyu, Adeyemi Odunola Tiamiyu, Muinat Usman, Yusuf Folorunsho Abdulkareem
    Journal of Crop Science and Biotechnology.2022; 25(2): 173.     CrossRef
  • Selection of superior cowpea lines for multi-traits and adaptabilities to the Piauí semi-arid using genotype by yield*trait biplot analysis
    Maurício dos Santos Araújo, Samíria Pinheiro dos Santos, Walter Frazão Lelis de Aragão, Kaesel Jackson Damasceno-Silva, Maurisrael de Moura Rocha
    Ciência e Agrotecnologia.2021;[Epub]     CrossRef
  • Cowpea development under different temperatures and carbon dioxide concentrations
    Francislene Angelotti, Laise Guerra Barbosa, Juliane Rafaele Alves Barros, Carlos Antonio Fernandes dos Santos
    Pesquisa Agropecuária Tropical.2020;[Epub]     CrossRef
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Positively Selected Orthologous Genes Identified in Sesame (Sesamum indicum) by Deep Resequencing
Jie Yu, Myeong-Hyeon Min, Sang-Ho Chu, Kyu-Won Kim, Yong-Jin Park
Plant Breed. Biotech. 2019;7(1):24-33.   Published online March 1, 2019
DOI: https://doi.org/10.9787/PBB.2019.7.1.24

Sesame (Sesamum indicum L.) is the queen of oil seed crops and is cultivated widely in tropical and subtropical areas. The availability of the sesame genome sequence presents unprecedented opportunities for studying its genetics, genomics, and evolution. In this report, we conducted a genome resequencing-based identification of sesame orthologs; in total, 26,379 coding sequences (CDSs) were isolated. Using a reciprocal best hit, we ultimately identified a total of 639 orthologs sets after one-to-one orthologs extraction across seven Pentapetalae plant species. These orthologs were considered to be the most credible between the two species, and in sesame. Furthermore, we performed a branch model-based maximum likelihood estimation of dN/dS of the orthologs, resulting in the identification of 198 evolutionarily accelerated orthologs and 66 positively selected genes (P-value and FDR < 0.05). An enrichment analysis and protein interaction network suggested 19 genes with important functions of the orthologs specific in sesame development and domestication. The method we used here provides a case study for identifying orthologous genes between sesame and other plants species that are distributed in equilibrium phylogenetically, which can be used in other plants.

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Evaluation of Yield Components from Transgenic Soybean Overexpressing Chromatin Architecture-Controlling ATPG8 and ATPG10 Genes
Hyun Suk Cho, Dong Hee Lee, Ho Won Jung, Seon-Woo Oh, Hye Jeong Kim, Young-Soo Chung
Plant Breed. Biotech. 2019;7(1):34-41.   Published online March 1, 2019
DOI: https://doi.org/10.9787/PBB.2019.7.1.34

AT-hook proteins are known to co-regulate transcription of genes through the modification of chromatin architecture. In plants, many genes encoding AT-hook proteins have been shown to be associated with increased seed yield or delayed senescence. In this study, we produced transgenic soybean plants overexpressing chromatin architecture-controlling ATPG8 and ATPG10 genes by Agrobacterium-mediated transformation and examined their agronomic traits to identify the yield increase in soybean crop similar to those seen in model plants, Arabidopsis. A total of 16 (3 of pB2GW7.0-ATPG8 and 13 of pCSEN-ATPG10 transformed) transgenic soybean plants were produced and their T1 seeds were harvested. Healthy and well-grown transgenic lines were selected (lines #1 and #2 from pB2GW7.0-ATPG8, and lines #8 and #9 from pCSEN-ATPG10), and the insertion and transcription level of genes were confirmed by PCR and RT-PCR with expected size. Investigation on agricultural traits confirms the increase in yield, plant height, the number of pods, and total seed weight with statistical significance when compared to wild-type soybean plants. The yield component study suggested that overexpression of ATPG8 and ATPG10 genes conferred positive effect on yield in transgenic soybean.

Citations

Citations to this article as recorded by  
  • Evolving role of synthetic cytokinin 6-benzyl adenine for drought stress tolerance in soybean (Glycine max L. Merr.)
    Phetole Mangena
    Frontiers in Sustainable Food Systems.2022;[Epub]     CrossRef
  • Mutation of GmIPK1 Gene Using CRISPR/Cas9 Reduced Phytic Acid Content in Soybean Seeds
    Ji Hyeon Song, Gilok Shin, Hye Jeong Kim, Saet Buyl Lee, Ju Yeon Moon, Jae Cheol Jeong, Hong-Kyu Choi, In Ah Kim, Hyeon Jin Song, Cha Young Kim, Young-Soo Chung
    International Journal of Molecular Sciences.2022; 23(18): 10583.     CrossRef
  • Comparative untargeted metabolomic analysis of Korean soybean four varieties (Glycine max (L.) Merr.) based on liquid chromatography mass spectrometry
    Eun-Ha Kim, Soo-Yun Park, Sang-Gu Lee, Hyoun-Min Park, Oh Suk Yu, Yun-Young Kang, Myeong Ji Kim, Jung-Won Jung, Seon-Woo Oh
    Journal of Applied Biological Chemistry.2022; 65(4): 439.     CrossRef
  • Overexpression of ATHG1/AHL23 and ATPG3/AHL20, Arabidopsis AT-hook motif nuclear-localized genes, confers salt tolerance in transgenic Zoysia japonica
    Ha-Na Jeong, Hyeon-Jin Sun, Zhi-Fang Zuo, Dong Hee Lee, Pill-Soon Song, Hong-Gyu Kang, Hyo-Yeon Lee
    Plant Biotechnology Reports.2020; 14(3): 351.     CrossRef
  • Increased Production of α-Linolenic Acid in Soybean Seeds by Overexpression of Lesquerella FAD3-1
    Wan Woo Yeom, Hye Jeong Kim, Kyeong-Ryeol Lee, Hyun Suk Cho, Jin-Young Kim, Ho Won Jung, Seon-Woo Oh, Sang Eun Jun, Hyun Uk Kim, Young-Soo Chung
    Frontiers in Plant Science.2020;[Epub]     CrossRef
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Phosphate is one of the major nutrients of growth, development, and reproduction of crop plants and functions in energy metabolism, signal transduction cascades, and regulates enzymatic activities. To understand uptake and usage of this nutrient in Oryza sativa (rice), a model crop plant, global studies on this family is more effective. Here, we conducted phylogenomic analyses of 26 rice and 19 Arabidopsis phosphate transporters (PHT) reported from previous studies, by integrating various meta-expression data to the phylogenic tree context. Subsequently, of four subfamilies, the PHT1 subfamily was a high affinity phosphate transporter, which functioned under low concentrations of phosphorous in soil, while the others (i.e., PHT2, PHT3, and PHT4) were low-affinity phosphate transporter subfamilies. Most members of the PHT1 in rice and Arabidopsis, in contrast to the other transporter subfamilies, showed significant induction under phosphate starvation, and the responses were more obvious in the roots. These results indicated that the functions of PHT1 phosphate transporters in rice and Arabidopsis were well conserved in response to phosphate starvation. We confirmed significant upregulation of seven PHT1 subfamily genes in rice under phosphate starvation, by RT-PCR, indicating that the high affinity phosphate transporters played important roles in the uptake of phosphate under phosphate deficiency. The regulatory network of OsPT4 belonged to the PHT1 subfamily based on RiceNet analysis, suggesting clues for further analyses. Our study showed the significance of at least seven PHT1 subfamily members, which could improve the efficiency of phosphate use in rice, as a model crop plant.

Citations

Citations to this article as recorded by  
  • Arbuscular Mycorrhizal Fungi Modulate Variety-Specific Phosphate Transporter Gene Expression in Aerobic Rice Under Phosphorus-Limited Soil Conditions
    Debasis Mitra, Periyasamy Panneerselvam, Parameswaran Chidambaranathan, Amaresh Kumar Nayak, Anjani Kumar, Upendra Kumar, Priyashree Parida, Abhishek Kumar Sahu, Annamalai Anandan, Pradeep Kumar Das Mohapatra
    Plants.2026; 15(11): 1675.     CrossRef
  • Functional Characterization of the VvPHT1 Gene and Its Promoter in Vicia villosa
    Shuqin Tang, Linlin Mao, Ruili Zhu, Moli Zheng, Shaojun Qiu, Dali Song, Jingwen Sun
    Agronomy.2026; 16(8): 824.     CrossRef
  • Identification and expression analysis of phosphate transporter (PHT) genes in Brachypodium distachyon in response to phosphorus deficiency
    Chiraz Friji, Hatem Boubakri, Luisa M. Martinez, Laura Ruiz Torres, Antonio José Manzaneda, Mhemmed Gandour
    Protoplasma.2025; 262(3): 515.     CrossRef
  • Enhanced use of chemical fertilizers and mitigation of heavy metal toxicity using biochar and the soil fungus Bipolaris maydis AF7 in rice: Genomic and metabolomic perspectives
    Arjun Adhikari, Eun-Hae Kwon, Muhammad Aaqil Khan, Shifa Shaffique, Sang-Mo Kang, In-Jung Lee
    Ecotoxicology and Environmental Safety.2024; 271: 115938.     CrossRef
  • Effects of different phosphorus levels on tiller bud development in hydroponic Phyllostachys edulis seedlings
    Chenjun Lin, Tingting Hang, Chenhao Jiang, Ping Yang, Mingbing Zhou, Michael J Considine
    Tree Physiology.2023; 43(8): 1416.     CrossRef
  • Comparative physiological, metabolomic, and transcriptomic analyses reveal mechanisms of apple dwarfing rootstock root morphogenesis under nitrogen and/or phosphorus deficient conditions
    Bin Xie, Yanhui Chen, Yanzhen Zhang, Xiuhong An, Xin Li, An Yang, Guodong Kang, Jiangtao Zhou, Cungang Cheng
    Frontiers in Plant Science.2023;[Epub]     CrossRef
  • Phosphorus homeostasis: acquisition, sensing, and long-distance signaling in plants
    V. Prathap, Anuj Kumar, Chirag Maheshwari, Aruna Tyagi
    Molecular Biology Reports.2022; 49(8): 8071.     CrossRef
  • Comparative in silico analysis of phosphate transporter gene family, PHT, in Camelina sativa gemome
    Sahar Faraji, Soosan Hasanzadeh, Parviz Heidari
    Gene Reports.2021; 25: 101351.     CrossRef
  • Expression of potential reference genes in response to macronutrient stress in rice and soybean
    Sandeep Sharma, Krishnapriya Vengavasi, M. Nagaraj Kumar, Shiv Kumar Yadav, Renu Pandey
    Gene.2021; 792: 145742.     CrossRef
  • A regulatory network driving shoot lignification in rapidly growing bamboo
    Kebin Yang, Lichao Li, Yongfeng Lou, Chenglei Zhu, Xueping Li, Zhimin Gao
    Plant Physiology.2021; 187(2): 900.     CrossRef
  • Phenylalanine ammonia-lyase family is closely associated with response to phosphate deficiency in rice
    Yun-Shil Gho, Sang-jin Kim, Ki-Hong Jung
    Genes & Genomics.2020; 42(1): 67.     CrossRef
  • Effect of phytochrome-mediated red light signaling on phosphorus uptake and accumulation in rice
    Yasuhito Sakuraba, Shuichi Yanagisawa
    Soil Science and Plant Nutrition.2020; 66(5): 745.     CrossRef
  • Overexpression of OsPT8 Increases Auxin Content and Enhances Tolerance to High-Temperature Stress in Nicotiana tabacum
    Zhaopeng Song, Ningbo Fan, Guizhen Jiao, Minghong Liu, Xiaoyan Wang, Hongfang Jia
    Genes.2019; 10(10): 809.     CrossRef
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Characterization of Chloroplast Genomes, Nuclear Ribosomal DNAs, and Polymorphic SSR Markers Using Whole Genome Sequences of Two Euonymus hamiltonianus Phenotypes
Junki Lee, Shin-Jae Kang, Hyeonah Shim, Sang-Choon Lee, Nam-Hoon Kim, Woojong Jang, Jee Young Park, Jeong Hwa Kang, Wan Hee Lee, Taek Joo Lee, Gyoungju Nah, Tae-Jin Yang
Plant Breed. Biotech. 2019;7(1):50-61.   Published online March 1, 2019
DOI: https://doi.org/10.9787/PBB.2019.7.1.50

Although genomics provides useful tools for crops, most wild resource plants still lack molecular data. To retrieve useful genomic data and thus provide fundamental information for a resource plant, we established a multi-directional approach using two low coverage whole-genome shotgun sequence (WGS) data of Euonymus hamiltonianus, which is a wild resource plant with potential as a medicinal and ornamental plant. We assembled complete chloroplast genome and nuclear ribosomal DNA (nrDNA) sequences and analyzed polymorphic simple sequence repeats (pSSRs) in the nuclear genome based on the comparison of WGS data between two different phenotypes. We developed a bioinformatics pipeline to identify pSSR motifs by systematic comparison of two WGS datasets. The pipeline is composed of multiple steps including end-joining of paired reads, isolation of joined reads harboring SSR motifs derived from unique non-repetitive regions, identification of pSSR via in silico comparison with counterpart WGS reads, design of pSSR primer sets, and validation. The pipeline was applied to WGS data of E. hamiltonianus and identified 161 contigs with pSSR motifs between the two different phenotypes. Based on the pSSR motifs, 20 primer pairs were designed, of which seven were successfully validated as real pSSR markers. We expect this information to be applicable to genomic resources of E. hamiltonianus.

Citations

Citations to this article as recorded by  
  • A First Approach for the In Vitro Cultivation, Storage, and DNA Barcoding of the Endangered Endemic Species Euonymus koopmannii
    Balnur Kali, Sara Bekkuzhina, Dilnur Tussipkan, Shuga Manabayeva
    Plants.2024; 13(16): 2174.     CrossRef
  • High-throughput discovery of plastid genes causing albino phenotypes in ornamental chimeric plants
    Hyun-Seung Park, Jae-Hyeon Jeon, Woohyeon Cho, Yeonjeong Lee, Jee Young Park, Jiseok Kim, Young Sang Park, Hyun Jo Koo, Jung Hwa Kang, Taek Joo Lee, Sang Hoon Kim, Jin-Baek Kim, Hae-Yun Kwon, Suk-Hwan Kim, Nam-Chon Paek, Geupil Jang, Jeong-Yong Suh, Tae-J
    Horticulture Research.2023;[Epub]     CrossRef
  • Analysis of the complete plastomes and nuclear ribosomal DNAs from Euonymus hamiltonianus and its relatives sheds light on their diversity and evolution
    Young Sang Park, Jong-Soo Kang, Jee Young Park, Hyeonah Shim, Hyun Ok Yang, Jung Hwa Kang, Tae-Jin Yang, Sudhindra R. Gadagkar
    PLOS ONE.2022; 17(10): e0275590.     CrossRef
  • Comparative Analyses of Euonymus Chloroplast Genomes: Genetic Structure, Screening for Loci With Suitable Polymorphism, Positive Selection Genes, and Phylogenetic Relationships Within Celastrineae
    Yongtan Li, Yan Dong, Yichao Liu, Xiaoyue Yu, Minsheng Yang, Yinran Huang
    Frontiers in Plant Science.2021;[Epub]     CrossRef
  • De Novo Assembly and Species-Specific Marker Development as a Useful Tool for the Identification of Scutellaria L. Species
    Hakjoon Choi, Wan Seok Kang, Jin Seok Kim, Chang-Su Na, Sunoh Kim
    Current Issues in Molecular Biology.2021; 43(3): 2177.     CrossRef
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Rapid Communications
Development of Kompetitive Allele Specific PCR Markers for Submergence Tolerant Gene Sub1 in Rice
Je-Hoon Moon, Daniel Son, Jung-Woo Lee, Soo-Cheul Yoo
Plant Breed. Biotech. 2019;7(1):62-66.   Published online March 1, 2019
DOI: https://doi.org/10.9787/PBB.2019.7.1.62

The SUBMERGENCE 1 (SUB1) locus, conferring tolerance to complete inundation, was identified, and gel-based DNA markers, AEX1 and GnS2, were previously developed for marker-assisted breeding (MAB). However, a high throughput and high specific method, at low cost, is still required to detect Sub1 alleles. Kompetitive Allele Specific PCR (KASP) markers enable high throughput analysis for a large number of seeds, as well as detection of both alleles, in a single reaction. In this study, we developed KASP markers that can distinguish specific alleles at Sub1A loci based on single nucleotide polymorphisms (SNPs). Marker validations were carried out by genotyping of a segregating population with the developed KASP markers. The results from KASP assay and gel-based marker analysis were consistent for Sub1A alleles. KASP markers developed for Sub1A would be helpful due to high accuracy, low cost, and a high throughput genotyping feature in MAB.

Citations

Citations to this article as recorded by  
  • Identification of KASP-SNP markers correlated with both growth and hypoxia tolerance traits in blunt snout bream (Megalobrama amblycephala)
    Yanluo Zhuo, Han Wang, Guanghai Dong, Huihu Wang, Guodong Zheng, Shuming Zou
    Aquaculture.2025; 608: 742745.     CrossRef
  • Unraveling the genetic enigma of rice submergence tolerance: Shedding light on the role of ethylene response factor-encoding gene SUB1A-1
    Md Ibrahim Khalil, Md Mahmudul Hassan, Swadesh Chandra Samanta, Abul Kashem Chowdhury, Md Zahid Hassan, Nasar Uddin Ahmed, Uzzal Somaddar, Sharmistha Ghosal, Arif Hasan Khan Robin, Ujjal Kumar Nath, Mohammad Golam Mostofa, David J. Burritt, Chien Van Ha,
    Plant Physiology and Biochemistry.2024; 206: 108224.     CrossRef
  • KASP: a high-throughput genotyping system and its applications in major crop plants for biotic and abiotic stress tolerance
    Bhawna Dipta, Salej Sood, Vikas Mangal, Vinay Bhardwaj, Ajay Kumar Thakur, Vinod Kumar, Brajesh Singh
    Molecular Biology Reports.2024;[Epub]     CrossRef
  • Development of SNP Marker Set to Select Varieties Tolerant to Multiple Abiotic Stresses in Rice
    Jung-Woo Lee, Jung-Seok Oh, Soo-Cheul Yoo
    Plant Breeding and Biotechnology.2023; 11(3): 208.     CrossRef
  • Development of Kompetitive Allele Specific PCR Markers for Anaerobic Germination 1 Locus in Rice
    Jung-Woo Lee, Joong Hyoun Chin, Soo-Cheul Yoo
    Plant Breeding and Biotechnology.2021; 9(1): 20.     CrossRef
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Development of Polymorphic SSR Markers from Pinus densiflora (Pinaceae) Natural Population in Korea
Hee Chung, Jaebok Lee, Jinsu Gil, Yurry Um, Ji Hyun Kim, Min Yeong Hwang, Ho Bang Kim, Chang Pyo Hong, Shin Gi Park, Donghwan Shim, Yi Lee
Plant Breed. Biotech. 2019;7(1):67-71.   Published online March 1, 2019
DOI: https://doi.org/10.9787/PBB.2019.7.1.67

Simple sequence repeat (SSR) markers were developed from Pinus densiflora, a species native to Asia, to investigate its genetic diversity and population structure in order to provide information for the management and breeding of this species. Using next-generation sequencing, a total of 1,008 putatively polymorphic SSR primer sets were designed. Seventeen polymorphic SSR markers in 121 individuals belonging to four natural populations of P. densiflora were identified and characterized, with three to seventeen alleles per locus. The expected heterozygosity ranged from 0.1844 to 0.8731 in four populations, and the average of the PIC values ranged from 0.2789 to 0.8488. Cross amplification of these markers was performed among the related species P. rigida, P. koraiensis, P. parviflora, and P. bungeana. The developed novel SSR markers are promising tools for studying the genetic diversity or population structure of P. densiflora and its related species.

Citations

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  • Analysis of the genetic diversity and population structure of Lindera obtusiloba (Lauraceae), a dioecious tree in Korea
    Ho Bang Kim, Hye-Young Lee, Mi Sun Lee, Yi Lee, Youngtae Choi, Sung-Yeol Kim, Jaeyong Choi
    Journal of Plant Biotechnology.2023;[Epub]     CrossRef
  • Genome-wide microsatellite characterization and marker development in Diospyros oleifera
    Yang Xu, Cuiyu Liu, Xu Yang, Kaiyun Wu, Bangchu Gong
    Industrial Crops and Products.2023; 203: 117182.     CrossRef
  • Genetic Diversity and Structure of Pinus densiflora Siebold & Zucc. Populations in Republic of Korea Based on Microsatellite Markers
    Ji-Young Ahn, Jei-Wan Lee, Kyung-Nak Hong
    Forests.2021; 12(6): 750.     CrossRef
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