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Volume 10(4); December 2022

Review Articles

Review on Mechanism of Mineral Phosphate Solubilization in Fast-Growing Rhizobia Based on Sugar Utilization
Bhumi R. Rajguru, Vaibhav D. Bhatt
Plant Breed. Biotech. 2022;10(4):203-211.   Published online December 1, 2022
DOI: https://doi.org/10.9787/PBB.2022.10.4.203

Plant Growth Promoting Rhizobacteria (PGPR) were used in many previous studies for increasing plant growth and productivity. Some mechanisms were used to enhance the soil productivity such as mineral phosphate solubilization, siderophore production, biological nitrogen fixation, induced systemic resistance and indole acetic acid (IAA) production. Some of the related researches adopted the phosphate solubilization in organisms which was repressed in the presence of succinate resembling the phenomenon of catabolite repression. In addition, the sugar utilization pattern (monosaccharide, disaccharide, and trisaccharide), organisms showed the characteristics like slow and fast-growing rhizobia respectively. Whole-genome sequencing has been used for identifying unique genes in Fast-growing Rhizobia. Existing literature in PGPR studies has been considered and mechanisms for increasing plant growth and productivity have been identified. The review tries to analyse the mechanism of phosphate solubilization and sugar utilization in fast growing Rhizobia. On the contrary, repression due to various carbon sources such as succinate has been reported, leading to succinate mediated catabolite repression.

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  • Population and Diversity of Pigeonpea Rhizobia in Vertisols of Central India
    Nagvanti Atoliya, Santosh Ranjan Mohanty, D. L. N. Rao
    Agricultural Research.2026; 15(2): 860.     CrossRef
  • Soil and Mineral Nutrients in Plant Health: A Prospective Study of Iron and Phosphorus in the Growth and Development of Plants
    Mujtaba Aamir Bhat, Awdhesh Kumar Mishra, Sheezma Nazir Shah, Mudasir Ahmad Bhat, Saima Jan, Safikur Rahman, Kwang-Hyun Baek, Arif Tasleem Jan
    Current Issues in Molecular Biology.2024; 46(6): 5194.     CrossRef
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Genetic Analysis of Coffee (Coffea arabica L.) Genotypes in Zimbabwe Using Morphological Traits
Pardon Chidoko, Caleb Mahoya, Samson Tarusenga, Dumisani Kutywayo
Plant Breed. Biotech. 2022;10(4):212-223.   Published online December 1, 2022
DOI: https://doi.org/10.9787/PBB.2022.10.4.212

The coffee value chain is a source of livelihood for millions of people across the world and yet the resilience of coffee is limited by the relatively narrow genetic base among commercial coffee cultivars. A study was conducted to determine genetic variation, heritability estimates and relationships among coffee genotypes in Zimbabwe. Quantitative morphological characteristics of twelve genotypes were recorded under field conditions. There were significant variations in coffee yield, plant height, stem girth, number of primary branches, number of bearing branches, internode length and leaf characteristics, with no significant variations in seed characteristics and number of nodes. Broad sense heritability estimates for the quantitative traits ranged from 0.03% to 91.4%, being highest for plant height, coffee yield, stem girth, leaf length and leaf area. The implications are that coffee yield and plant height are independent of significant environmental influences while seed, branching traits and leaf traits are influenced by the environment in their expression. Yield was significantly correlated to branches per plant, plant height, seed traits and stem girth. Clustering of genotypes was influenced by plant height, yield and stem girth. Overall, few traits were important in distinguishing coffee genotypes, implying narrow diversity. Hybridization, further introductions from other producer countries, coffee gene banks and/or introductions from the wild, and concerted germplasm conservation efforts are recommended.

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  • Structural Equation Modeling and Genome-Wide Selection for Multiple Traits to Enhance Arabica Coffee Breeding Programs
    Matheus Massariol Suela, Camila Ferreira Azevedo, Ana Carolina Campana Nascimento, Eveline Teixeira Caixeta Moura, Antônio Carlos Baião de Oliveira, Gota Morota, Moysés Nascimento
    Agronomy.2025; 15(7): 1686.     CrossRef
  • Towards a minimum number of key flower traits in studies of Coffea spp. phenotype variability
    Larícia Olária Emerick Silva, Maria Juliete Lucindo Rodrigues, Rafael Nunes de Almeida, José Nobre Semedo, Miroslava Rakocevic, Fábio Luiz Partelli
    Scientia Horticulturae.2024; 337: 113513.     CrossRef
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Research Articles
Effect of Wheat RING Type E3 Ligase TaMARCH9 Gene on Flowering Time in Transgenic Arabidopsis
Moon Seok Kim, Jae Ho Kim, Yong Weon Seo
Plant Breed. Biotech. 2022;10(4):224-231.   Published online December 1, 2022
DOI: https://doi.org/10.9787/PBB.2022.10.4.224

Grain yield in wheat is mostly governed prior to flowering during primordia development. Mature primordia development, floret fertility, and maximum grain number per spikelet are the three most important characteristics of spike development. The genes involved in primordia formation, on the other hand, have remained unclear. Here, we demonstrate molecular characterization of Triticum aestivum MARCH9 (TraesCS7A01G283700) which encodes RING domain, might play a positively regulating primordium and floret development until anthesis stages. TaMARCH9 expression level was highly induced at differentiation stages (WS 3-6) in Yeongkwang cultivar. TaMARCH9 exhibited ubiquitination activities and was localized to the plasma membrane using a transient expression system in tobacco. Moreover, overexpression of TaMARCH9 in Arabidopsis plant displayed early flowering phenotypes compared to wild-type. Some of flowering related genes such as FLC, MAF, FT and SOC were highly induced in TaMARCH9- overexpressing Arabidopsis plants. Our finding suggested that TaMARCH9 may act a key role in the formation of floret primordium development and/or flowering time in wheat.

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Estimation of Heritability and Association Analysis of Agronomic Traits Contributing to Yield on Upland Rice (Oryza sativa L.)
Dewi Nawank Sary, Lailatul Badriyah, Ricoh Darisman Sihombing, Thoriq Ahmad Syauqy, Eries Dyah Mustikarini, Gigih Ibnu Prayoga, Ratna Santi, Budi Waluyo
Plant Breed. Biotech. 2022;10(4):232-243.   Published online December 1, 2022
DOI: https://doi.org/10.9787/PBB.2022.10.4.232

Rice (Oryza sativa L.) is an important commodity with a major influence on the country's economy. Plant breeding activities must be conducted to develop high-yielding potential genotypes with desired agronomic traits. The purpose of this research is to study the heritability and coheritability of agronomic traits as well as to study the direct and indirect effects through genotypic and phenotypic correlations among agronomic traits contributing to yield on genotypes of rice. The research was carried out from March to July 2022 at the Experimental Field of the Faculty of Agriculture, Brawijaya University, Jatimulyo, Lowokwaru, Malang, East Java. Ten genotypes of rice were used as genetic materials. The research used a Randomized Block Design (RBD) method with 3 replications. Data were analyzed using analysis of variance, analysis of covariance, and path analysis. The results revealed that there were very significant differences among upland rice genotypes for all observed traits. All observed agronomic traits had high heritability estimates. Plant height with time of inflorescence emergence had the highest coheritability, and flag leaf length with yield per plot had the lowest coheritability. The traits of the number of spikelets per panicle and weight of milled dry grains positively correlated with yield. Weight of milled dry grains had the highest positive direct effect through genotypic correlation and phenotypic correlation with yield.

Citations

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  • Development of Hybrid Pleurotus cystidiosus Strains with Enhanced Functional Properties
    Sung-I Woo, Minji Oh, Hak Hyun Lee, Inseo Song, Se Jeong Kim, Youn-Lee Oh, Ji-Hoon Im, Eun-Ji Lee, Mi Kyeong Lee
    Foods.2025; 14(24): 4329.     CrossRef
  • Comprehensive evaluation of nitrogen fertilization impact on early maturing rice varieties using multivariate analysis and vegetation indices
    Yunus Musa, Rusnadi Padjung, Nasaruddin Nasaruddin, Muh Farid, Andang Suryana Soma, Achmad Kautsar Baharuddin, Muh. Fikri Al Qautzar, Resky Maulidina Fakhri, Madonna Casimero, Amin Nur, Mahmoud F. Seleiman, Majed Alotaibi, Nawab Ali, Muhammad Fuad Anshori
    Frontiers in Sustainable Food Systems.2025;[Epub]     CrossRef
  • Morphoagronomical Evaluation of Several Indonesian Pigmented Rice (Oryza sativa L.) Accessions from East Java and Central Java, Indonesia
    Yeni Avidhatul Husnah, Turhadi Turhadi, Anna Safitri, Fatchiyah Fatchiyah
    Plant Breeding and Biotechnology.2024;[Epub]     CrossRef
  • Genomic prediction of cereal crop architectural traits using models informed by gene regulatory circuitries from maize
    Edoardo Bertolini, Mohith Manjunath, Weihao Ge, Matthew D Murphy, Mirai Inaoka, Christina Fliege, Andrea L Eveland, Alexander E Lipka, J Endelman
    GENETICS.2024;[Epub]     CrossRef
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The Effect of Growth Concentration on In Vitro Shoot Multiplication of Crown Flower (Calotropis gigantea)
Okky Talitha, Samanhudi Samanhudi, Andriyana Setyawati, Muji Rahayu, Amalia T. Sakya
Plant Breed. Biotech. 2022;10(4):244-256.   Published online December 1, 2022
DOI: https://doi.org/10.9787/PBB.2022.10.4.244

Crown flower (Calotropis gigantea) as an herbaceous plant, is wildly recognized for its benefits as a medicinal plant. Phytochemical compounds which are contained in leaves, roots, and flowers of crown flower can be used as medicines. However, crown flower in Indonesia is still not optimal in their utilization. The study aimed to obtain the right concentration of growth regulators Benzyl Amino Purine and Indole Butyric Acid to stimulate optimal growth and secondary metabolites of crown flower. The study used a Completely Randomized Design arranged in a factorial design. The first factor was Benzyl Amino Purine and the second was Indole Butyric Acid. The data obtained were analyzed using analysis of variance with a 5% level test. The interaction of Benzyl Amino Purine and Indole Butyric Acid significantly increased the number of leaves and shoot emergence time. However, medium without Benzyl Amino Purine improved the percentage of root emergence by 100%, the number of roots by 17.2 roots, and plantlet height by 7.45 cm. Optimal concentration was obtained in 3 ppm Benzyl Amino Purine, which produced the high shoot and emergence time, as well as the number of shoots and leaves. Higher flavonoid contents were contained in cultured plants than in conventional cultivation. Optimal growth and phytochemical content give an opportunity to increase the production and the use of crown flower as medicinal plant.

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  • An update on biotechnological intervention mediated by plant tissue culture to boost secondary metabolite production in medicinal and aromatic plants
    Shera Pandit Prashant, Mishra Bhawana
    Physiologia Plantarum.2024;[Epub]     CrossRef
  • Establishment and Validation of a Robust Reversed-Phase HPLC Method for the Determination of Calotropis gigantea in Bulk Material and Marketed Product
    Akshata P. Hebballi, Basavaraja Pujar, Suresh S. Honnalli, Sushmita I. Hiremath, Akshata Menasinakai, Akash Bakale, Sahil Nadaf, Ashika M.V., Sanjana Vadavalli
    Current Pharmaceutical Analysis.2024; 20(8): 920.     CrossRef
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Association Study for Drought Tolerance of Flint Maize Inbred Lines Using SSR Markers
Kyu Jin Sa, Hyeon Park, Zhenyu Fu, So Jung Jang, Ju-Kyong Lee
Plant Breed. Biotech. 2022;10(4):257-271.   Published online December 1, 2022
DOI: https://doi.org/10.9787/PBB.2022.10.4.257

Drought tolerance is derived from complex quantitative traits that are associated with different shoot and root morphological characters. This study assessed the genetic and phenotypic variation of 12 maize inbred lines and performed association analysis of 11 drought-related traits using 360 simple sequence repeats (SSRs), detecting 1,604 alleles, with an average of 4.4 alleles per locus. The average values of gene diversity (GD) and polymorphism information content (PIC) were 0.648 and 0.598, respectively. In principal component analysis (PCA), shoot fresh weight (SFW), shoot dry weight (SDW), stem weight (SW), leaf weight (LW), root fresh weight (RFW), root dry weight (RDW), and leaf area (LA) traits contributed greatly to the PCA. Association analysis was performed using a general linear model with a Q-matrix (Q GLM) and a mixed linear model with Q and K-matrices (Q + K MLM). Twelve SSR markers for drought tolerance trait were detected by Q GLM, and all maize inbred lines were clearly divided into two groups in accordance with their drought tolerance. Duplicated significant marker-trait associations (SMTAs) between Q GLM and Q + K MLM identified eight marker-trait associations involving four SSR markers that were associated with the traits of SW, SFW, RFW, and RDW with a significant level of P < 0.05. The umc1175 and umc2092 were associated with SW and SFW; umc1503 was associated with RFW, SFW, and SW; and umc2341 was associated with RDW. The detection of loci associated with drought-related traits in this study may provide better opportunities to improve maize drought tolerance by marker-assisted selection (MAS).

Citations

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  • Using Flint Maize for Developing New Hybrids: A Case Study in Romania
    Roxana Elena Călugăr, Andrei Varga, Carmen Daniela Vana, Loredana Ancuța Ceclan, Felicia Chețan, Andras Fodor, Nicolae Tritean
    Agronomy.2025; 15(9): 2215.     CrossRef
  • Leveraging Multiomics Insights and Exploiting Wild Relatives’ Potential for Drought and Heat Tolerance in Maize
    Shakra Jamil, Shakeel Ahmad, Rahil Shahzad, Noroza Umer, Shamsa Kanwal, Hafiz Mamoon Rehman, Iqrar Ahmad Rana, Rana Muhammad Atif
    Journal of Agricultural and Food Chemistry.2024; 72(29): 16048.     CrossRef
  • Association Mapping for Evaluation of Population Structure, Genetic Diversity, and Physiochemical Traits in Drought-Stressed Maize Germplasm Using SSR Markers
    Muhammad Zahaib Ilyas, Hyeon Park, So Jung Jang, Jungeun Cho, Kyu Jin Sa, Ju Kyong Lee
    Plants.2023; 12(24): 4092.     CrossRef
  • Uncovering microsatellite markers associated with agronomic traits of South Sudan landrace maize
    Emmanuel Andrea Mathiang, Hyeon Park, So Jung Jang, Jungeun Cho, Tae Hyeon Heo, Ju Kyong Lee
    Genes & Genomics.2023; 45(12): 1587.     CrossRef
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Inheritance of Resistance to Race 5 of Powdery Mildew Fungus Podosphaera xanthii in Melon and Development of Race 5-Specific High Resolution Melting Markers
Jeong-Eui Hong, Mohammad Rashed Hossain, Hee-Jeong Jung, Ill-Sup Nou
Plant Breed. Biotech. 2022;10(4):272-281.   Published online December 1, 2022
DOI: https://doi.org/10.9787/PBB.2022.10.4.272

Powdery mildew (PM), caused by the biotrophic fungus Podosphaera xanthii, drastically reduces the yield and quality of melon (Cucumis melo L.). Knowledge of the genetic control and high throughput molecular markers linked with resistance against this disease are essential for breeding programs. The bioassay study of the F1 and F2 populations derived from the parents, ‘PMR 5’ (♂) and ‘SCNU1154’ (♀) revealed a monogenic dominant nature of resistance to the devastating race, race 5. Besides, we developed three SNP based high resolution melting markers, PMm-HRM-1, PMm-HRM-2, and PMm-HRM-3, based on the previously identified SNPs on chromosome 12 and validated them using 8 melon lines and 137 F2 populations. Among these, the SNP of marker PMm-HRM-1 causes a missense mutation in the LRR region of MELO3C002393 and we were able to distinguish the resistant vs susceptible genotypes from eight diverse melon accessions and the segregating F2 population with more than 90% genotyping efficiency. The other two markers were based on intergenic SNPs and had more than 80% genotyping efficiency in F2 population. These markers will be helpful to melon breeders to develop melon cultivars resistant to P. xanthii race 5 via marker assisted breeding programs.

Citations

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  • Developing DNA Markers based on Male-Specific Chromosomal Regions for Selecting Male Plants in Hop (Humulus lupulus)
    Tae hyun Ha, Jae Il Lyu, So Young Yi, Si-Yong Kang
    Plant Breeding and Biotechnology.2024;[Epub]     CrossRef
  • Identification of Gene Responsible for Conferring Resistance against Race KN2 of Podosphaera xanthii in Melon
    Sopheak Kheng, San-Ha Choe, Nihar Sahu, Jong-In Park, Hoy-Taek Kim
    International Journal of Molecular Sciences.2024; 25(2): 1134.     CrossRef
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Identification of QTLs for Cold Tolerance at Seedling Stage Using a Population Derived from an Inter-specific Cross in Rice
Kyu-Chan Shim, Yeo-Tae Yun, Ju-Won Kang, Sang-Nag Ahn
Plant Breed. Biotech. 2022;10(4):282-289.   Published online December 1, 2022
DOI: https://doi.org/10.9787/PBB.2022.10.4.282

Cold stress is one of the serious abiotic stresses for stable rice production especially in high-latitude temperate region and high-altitude tropical area. Improving cold tolerance at seedling stage led stable seedling growth with yield stability. In this study, QTLs for cold tolerance at seedling stage were identified using the 96 introgression lines (ILs) derived from an inter-specific cross between Hwaseong (Oryza sativa) and Oryza rufipogon. Three QTLs were detected and the O. rufipogon alleles at two QTL (qCTS1 and qCTS12) improved cold tolerance in the Hwaseong genetic background whereas the O. rufipogon allele at qCTS10 on chromosome 10 decreased cold tolerance. Among these three QTLs, a major QTL qCTS12 explained 27.5% of phenotypic variation. Fine-mapping indicated that qCTS12 was different from those QTL reported in previous studies based on the map location suggesting that qCTS12 might be a new allele and is not associated with deleterious genes such fertility reduction. Among the 96 introgression lines, two lines, CR60 and CR61 were selected based on enhanced cold tolerance at seedling stage. qCTS12, therefore, provides a valuable allele for breeding rice with improved cold tolerance.

Citations

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  • Stage-specific screening reveals differential resilience response to cold stress in rice
    Fahamida Akter, Partha S. Biswas, Abul Kalam Mohammad Aminul Islam, Mohammad Sharif Raihan, Md. Mizanur Rahman, Khandakar Md. Iftekharuddaula, Mohammad Rafiqul Islam, John Damien Platten, Md Ashrafuzzaman
    PLOS One.2026; 21(4): e0338290.     CrossRef
  • Genome-wide Association Study for Cold Tolerance at Seedling Stage Using a Core Collection of Korean Rice
    Sa-Eun Park, Ngoc Ha Luong, Sang-Nag Ahn, Kyu-Chan Shim
    Journal of Agriculture & Life Science.2025; 59(2): 13.     CrossRef
  • Identification of QTLs Related to Plant Growth at Low Temperatures in the Seedling Stage of Tongil Type Rice after Transplanting
    Seong-Gyu Jang, Ji-Yoon Lee, Ju-Won Kang, Youngho Kwon, So-Myeong Lee, Sais-Beul Lee, Jun-Hyeon Cho, Dong-Soo Park, Jong-Hee Lee, Soon-Wook Kwon, Sumin Jo
    Korean Journal of Breeding Science.2024; 56(3): 225.     CrossRef
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