Boysenberry, a
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With the continual development of genetically modified (GM) crops, it has become necessary to develop detailed and effective molecular characterization methods to select candidate events from a large pool of transformation events. Relative to traditional molecular analysis methods such as the polymerase chain reaction (PCR) and Southern blot hybridization, next generation sequencing (NGS) technology for whole-genome sequencing of complex crop genomes had proven comparatively useful for in-depth molecular characterization. In this study, four transformation events, including one in
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Dehydrins (DHNs) are hydrophilic proteins with conserved lysine-rich K-segment, which belong to Group II of the late embryogenesis abundant (LEA) protein family. DHNs are considered as molecular chaperons playing important roles in abiotic stress tolerance. In this study,
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Seed starch content (SSC) is a decisive factor influencing soy food quality. Variation in SSC affects the composition of major components, oil, and protein in soybean seeds. Therefore, understanding G × E interaction of SSC is important to produce soybeans with stable SSC. In the present study, G × E interactions of 17 soybean genotypes having different SSC (0.24–1.48%) and correlation of SSC with crude protein (CP) and crude fat (CF) were investigated. The genotypes were evaluated for SSC and other traits at two planting dates across three locations over two years (2015 and 2016). The genotype × year, genotype × location, and genotype × year × location interactions were found to be significant (
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The faba bean, one of the few crops that produces L-3,4-dihydroxy-phenylalanine (L-dopa) naturally, is also a good source of fatty acids and isoflavones. This study analyzed fatty acids, L-dopa and isoflavones in the leaves, immature pods and seeds of selected high-yielding faba bean genotypes spring-sown in Korea. The most abundant fatty acids in the leaves, immature pods and seeds were linolenic acid (58.6–63.7%), linoleic acid (46.9–58.9%), and linoleic acid (45.7–63.6%), respectively. The L-dopa content was highest in the leaf for all the faba bean genotypes except PI430715 and PI614810 where it was highest in the immature pods. The α-linolenic acid and L-dopa contents of the seed and immature pod of the PI430715 and PI614810 genotypes were the highest of all the genotypes. Four isoflavones (daidzin, genistin, daidzein and genistein) were detected with levels of daidzin and genistin generally higher than those of daidzein and genistein in each part of the faba bean. The L-dopa content was correlated (
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The
objective
of this study was to develop high-throughput SNP or SNP-based markers by re-sequencing of two peanut cultivars, ‘K-Ol’ and ‘Pungan’. The whole genome re-sequencing for the two cultivars was performed to produce sequences of 35.3 × 109 bp with 350 × 106 reads and 32.0 × 109 bp with 318 × 106 reads, respectively. As compared with the peanut reference genome, the distribution of homozygous and heterozygous SNPs on each chromosome showed very similar patterns between ‘K-Ol’ and ‘Pungan’, and most of them were in intergenic-region regardless of the peanut cultivars and reference genome type. The SNPs identified between the two peanut cultivars were evenly distributed across chromosomes of peanut diploid A and B reference genomes. It indicated that these SNPs could be available to construct a genetic map using the segregating population derived from a cross between ‘K-Ol’ and ‘Pungan’. Total 61 CAPS marker were developed and tested for their availability. Of the CAPS markers, 60 CAPS markers produced normal PCR products and 18 out of them presented polymorphism among 6 peanut varieties. Results of the present study could provide useful genetic resources to facilitate marker-assisted selection for breeding programs as well as germplasm screening for peanut.
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Heat stress is one of the factors disturb productivity and growth of plants. Many genes including heat shock protein (HSP), heat shock transcription factors (HSF) and chaperones, were identified and characterized in many plants to play role in increased tolerance to abiotic stress. To reveal responsive gene to heat stress, we performed RNA-seq using two Korean soybean varieties under heat stress and normal conditions. The transcripts were analyzed, and we obtained 2,458 genes including 46 co-up regulation and 55 co-down regulated genes in both soybean varieties. We also revealed HSPs, HSFs and chaperones in the differentially expressed genes using BLAST and Pfam analyzation and verified expression changes under heat stress. Finally, we find 68 genes involved in HSP, HSF, chaperones in heat responsive genes associated increasing heat tolerance. As a result, relatively small HSP families were up regulated and continuously expressed in long period heat stress. On the other hand, large molecule HSPs, HSFs and chaperonin did not response to long heat stress. The expression profiling and characterization provide invaluable information to understand heat tolerance of soybean.
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Grain size and panicle architecture are important traits determining yield. Here we report a new allele of
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In this study,
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Supplying sufficient rice to growing populations is a global challenge. Hybrid
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