This study was carried out to evaluate the genetic diversity and relationships among fifty-six blackberry (Rubus fructicosus) mutants derived from gamma-ray treatment (fifty-two lines) by analysis of Amplified fragment length polymorphism (AFLP) markers. Both cluster analysis and principal coordinate analysis (PCOORDA) were conducted in order to study the genetic diversity, using both morphological traits and AFLP makers. A total of 589 bands were amplified with an average of 58.9 bands per primer. Among them, 560 were identified to be polymorphic, with a rate of 95.08%. A showed a highly significant (P≤0.01) positive correlation with GD and PIC (r²=0.999). MI also showed a significant (P≤0.05) positive correlation with GD and PIC. According to the clustering analysis, all mutant lines could be classified into five categories, but the three gamma-ray treatment lines and the cross-bred line were not clustered into any groups. For the morphological traits, cluster analysis divided the blackberry germplasm into six clusters and two independent groups. In addition, the morphological dendrogram indicated an unclear pattern of division among the groups based on AFLP analysis. The findings of this study indicate that mutant lines have high genetic diversity, and can be effectively utilized as materials for the improvement of breeding.

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• Development of new genomic resources and tools for molecular breeding in blackberry
  M.L. Worthington, R. Aryal, N.V. Bassil, D. Mead, G.E. Fernandez, J.R. Clark, F. Fernández-Fernández, C.E. Finn, K.E. Hummer, H. Ashrafi
  Acta Horticulturae.2020; (1277): 39.     CrossRef
• Genotyping-by-sequencing based single nucleotide polymorphisms enabled Kompetitive Allele Specific PCR marker development in mutant Rubus genotypes
  Jaihyunk Ryu, Woon Ji Kim, Juhyun Im, Sang Hun Kim, Kang-Seop Lee, Han-Jig Jo, Ee-Youb Kim, Si-Yong Kang, Jeong-Hee Lee, Bo-Keun Ha
  Electronic Journal of Biotechnology.2018; 35: 57.     CrossRef

Amplified fragment length polymorphism (AFLP) is a molecular marker technique based on DNA and is extremely useful in detection of high polymorphism between closely related genotypes like Korean wheat cultivars. Six sequence characterized amplified regions (SCARs) have been developed from inter simple sequence repeat (ISSR) analysis which enabled the identification and differentiation of 13 Korean wheat cultivars from the other cultivars. We used six combinations of primer sets in our AFLP analysis for developing additional cultivar-specific markers in Korean wheat. Fifty-eight of the AFLP bands were isolated from EA-ACG/MA-CAC, EA-AGC/MA-CTG and EA-AGG/MA-CTA primer combinations. Of which 40 bands were selected to design SCAR primer pairs for Korean wheat cultivar identification. Three of 58 amplified primer pairs, KWSM006, KWSM007 and JkSP, enabled wheat cultivar identification. Consequently, 23 of 32 Korean wheat cultivars were classified by eight SCAR marker sets.

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• High-throughput SNP markers for authentication of Korean wheat cultivars based on seven complete plastomes and the nuclear genome
  Woohyeon Cho, Jin-Kee Jung, Min-Young Kang, Yong Weon Seo, Jee Young Park, Tae-Jin Yang
  Food Science and Biotechnology.2022; 31(4): 423.     CrossRef
• Development of CACTA transposon derived SCAR markers and their use in population structure analysis in Zea mays
  Neha Samir Roy, Kyong-Cheul Park, Sung-Il Lee, Min-Ji Im, Rahul Vasudeo Ramekar, Nam-Soo Kim
  Genetica.2018; 146(1): 1.     CrossRef
• Development of a SCAR marker associated with salt tolerance in durum wheat (Triticum turgidum ssp. durum) from a semi-arid region
  Sang Heon Kim, Jae Yoon Kim, Dae Yeon Kim, Jin Seok Yoon, Woo Joo Jung, Inés Yacoubi, Yong Weon Seo
  Genes & Genomics.2016; 38(10): 939.     CrossRef