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"Antioxidant"

Research Articles
Validation of Molecular Markers for Breeding High Antioxidant Traits in Thai Rice
Phanomsak Tomjai, Chanita Paliyavuth, Supachitra Chadchawan, Pimsiri Tiyayon, Papanee Anantasri, Waraluk Kasettranan
Plant Breed. Biotech. 2025;13:176-195.
Published online September 2, 2025
DOI: https://doi.org/10.9787/PBB.2025.13.176

This research attempted to validate novel molecular markers linked to high antioxidant traits using an F2 population and a local Thai rice population. We performed total flavonoid content, total phenolic content (TPC), and ferric reducing antioxidant power (FRAP) assays to assess the antioxidant capacities of rice populations. In the F2 (“Pathum Thani 1”דHawm Mali Daeng”) population, these traits exhibited a quantitative distribution with high heritability (82.7%–98.3%) and high genetic advance (66%–94%). In validation using 10 molecular markers, OsRc and In1-Rc displayed polymorphism in parent cultivars and in bulked segregant analysis. However, only OsRc had strong associations with TPC (R2 = 52.2%, p < 0.001) and FRAP (R2 = 43.0%, p < 0.001), whereas In1-Rc featured no significant associations (R2 ≤ 0.1%). When verified in Thai rice cultivars, OsRc could discriminate red pericarp rice cultivars with high antioxidant properties from white pericarp cultivars with low antioxidant activity, confirming its efficiency in selecting antioxidant traits in rice. These findings indicate that OsRc is a functional marker for selecting rice lines with high antioxidant activity, thereby facilitating early-stage genotyping in rice breeding programs.

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  • Genome-wide association study of antioxidant compounds and antioxidant activity in a panel of Thai rice cultivars
    Saranyu Thaworn, Phanomsak Tomjai, Teerapong Buaboocha, Supachitra Chadchawan, Monnat Pongpanich, Chanita Paliyavuth, Waraluk Kasettranan
    Euphytica.2026;[Epub]     CrossRef
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Phytochemical Screening, Antioxidant and Antibacterial Activity of Six Medicinal and Aromatic Plants in Morocco: A Comparative Study
Aymane Bessi, Chaimae Rais, Chaimae Slimani, Laila Elhanafi, Ikrame Zeouk, Boutaina Louafi, Lahsen El Ghadraoui, El Ouazna Bouchamma
Plant Breed. Biotech. 2025;13:15-32.
Published online February 17, 2025
DOI: https://doi.org/10.9787/PBB.2025.13.15

This study evaluates the phenolic content, antioxidant, and antibacterial properties of hydro-ethanolic extracts from six medicinal and aromatic plants in Morocco: Eucalyptus torquata, Thymus broussonetii, Lavandula angustifolia, Rosmarinus officinalis, Ziziphus lotus, and Acacia raddiana. Sonication was employed for extraction. Total phenolic content, total flavonoid content, and condensed tannin content were quantified using the Folin-Ciocalteu, aluminum chloride, and hydrochloric acid assays, respectively. Antioxidant capacity was assessed via DPPH, total antioxidant capacity, ferric reducing antioxidant power, and ABTS assays. Antibacterial activity was measured by the microdilution method. Phytochemical analysis revealed that E. torquata and Z. lotus had the highest total phenolic content, with 86.75 and 87.58 mg GAE/g of dry matter, respectively. T. broussonetii and R. officinalis had the highest flavonoid and condensed tannin content, at 297.12 mg QE/g of dry matter and 5.10 g/L, respectively. E. torquata exhibited the strongest antioxidant activity, with DPPH and ABTS values of 0.048 and 0.11 mg/mL, and a ferric reducing antioxidant power value of 209.375 mg/g. R. officinalis showed the highest total antioxidant capacity at 1.03 mg AAE/g of dry matter. All extracts displayed bactericidal effects. R. officinalis demonstrated the most potent antibacterial activity, with minimum inhibitory concentrations ranging from 0.625 to 5 mg/mL and minimum bactericidal concentrations from 1.25 to 10 mg/mL. High phenolic content and significant antioxidant activity were noted in E. torquata and R. officinalis. Additionally, all extracts showed substantial antibacterial activity against four bacterial strains, with R. officinalis being the most effective.

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Comparison of Antioxidant Components and Activities of Korean Black Soybeans
Hye Rang Park, Jeong Hyun Seo, Beom Kyu Kang, Jun Hoi Kim, Su Vin Heo, Yeong Hoon Lee, Won Young Han, Myung Chul Seo, Gi Rim Park
Plant Breed. Biotech. 2024;12:175-192.   Published online October 28, 2024
DOI: https://doi.org/10.9787/PBB.2024.12.175

Black soybeans are valued for their rich nutritional content and potential health benefits, attributed to their functional components that enhance antioxidant activity. In this study, we evaluate and compare the isoflavone and anthocyanin content, as well as the antioxidant potential, of seven Korean black soybean genotypes. Isoflavone content ranged from 2,032.8 to 3,536.8 µg/g, with Soman displaying the highest levels of both aglycones and glucosides, indicating notable bioactive potential. In terms of anthocyanins, Danheuk had the highest total content (24,080.6 µg/g), while Soman excelled in Pelargonidin-3- glucoside (Pg3glc). Soman also showed superior antioxidant activity across all measures, including total polyphenol, flavonoid content, as well as radical scavenging abilities (ABTS and DPPH). Strong correlations were found between total flavonoid content, total polyphenol content, genistin, total isoflavone content and antioxidant activity, while correlations with total anthocyanins were relatively weaker. These findings reveal significant genetic variability in isoflavone and anthocyanin content among soybean genotypes, with Soman showing particularly high antioxidant potential, suggesting its value for health-related applications and soybean breeding programs.

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  • Health benefits of Cyanidin-3-O-glucoside and other supporting natural compounds in black soybean (Glycine max (L.) Merrill): A narrative review
    Chiew Erh Jacqueline Moey, Wai Kwan Lau
    Journal of Functional Foods.2026; 140: 107285.     CrossRef
  • Enhanced Antitumor Activity of Korean Black Soybean Cultivar ‘Soman’ by Targeting STAT-Mediated Aerobic Glycolysis
    Su Hwan Park, Jeong Hyun Seo, Min Young Kim, Hye Jin Yun, Beom Kyu Kang, Jun Hoi Kim, Su Vin Heo, Yeong Hoon Lee, Hye Rang Park, Man Soo Choi, Jong-Ho Lee
    Antioxidants.2025; 14(2): 228.     CrossRef
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Root Development and Anti-Oxidative Response of Rice Genotypes under Polyethylene Glycol Induced Osmotic Stress
Juthy Abedin Nupur, Afsana Hannan, Md. Abir Ul Islam, G H M Sagor, Arif Hasan Khan Robin
Plant Breed. Biotech. 2020;8(2):151-162.   Published online June 1, 2020
DOI: https://doi.org/10.9787/PBB.2020.8.2.151

Osmotic stress is a kind of stress which is directly or indirectly related to all other abiotic stresses. Four rice varieties namely Binadhan-11 (with SUB1 gene), BRRI dhan52 (with SUB1 gene), Binadhan-7 and BRRI dhan71 were used to study the variation in root development and anti-oxidative response under osmotic stress conditions. Osmotic stress was induced by applying polyethylene glycol (4% PEG) in hydroponic solution. Treatment was given at the panicle initiation stage and leaf samples were collected at fourteen days after treatment to estimate antioxidant response in terms of ascorbate (APX) and peroxidase (POD) enzymatic activity. Roots were destructively harvested at 16 days after the stress imposition. The tested varieties showed significant differences in antioxidant responses under the stress condition such as high APX and POD in Binadhan-11 and BRRI dhan52. Significant injury at the 4th leaf position (the youngest leaf was the reference) was observed at 8 and 12 days after the treatment. Number of live leaves, shoot dry weight, density of second order lateral roots, number of phytomer (Pr) and total roots, number of roots at Pr2, main axis diameter at Pr1, main axis length at Pr1 varied significantly among the varieties × treatment combinations. Binadhan-11 and BRRI dhan52 showed comparatively higher osmotic stress tolerance compared to the other two varieties without SUB1 gene, viz., Binadhan-7 and BRRI dhan71. The varieties BRRI dhan52, and Binadhan-11 showed greater capacity to withstand osmotic stress can be further used to develop stress tolerant variety.

Citations

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  • Salinity and Polyethylene‐Glycol (PEG)‐Induced Osmotic Stress Alters the Early Vegetative Shoot and Root Morphology of Sweet Potato Genotypes in Hydroponic Culture
    Rebeka Sultana, Nikunjo Chakroborty, Jannatul Afrin, Yousuf Ali, Sarah Jabeen Mou, Jobadatun Naher, Arif Hasan Khan Robin
    New Zealand Journal of Botany.2026;[Epub]     CrossRef
  • Oxidative Stress in Rice (Oryza sativa): Mechanisms, Impact, and Adaptive Strategies
    Lady Edlenill J. Tavu, Mark Christian Felipe R. Redillas
    Plants.2025; 14(10): 1463.     CrossRef
  • Evaluating Wheat Yield and Nutrient Accumulation in Response to Foliar Application of Moringa Leaf Extract
    Israr Ahmed Baladi, Naheed Akhter Talpur, Zia-ul-Hassan Shah, Javaid Ahmed Shah, Khalid Hussain Talpur, Javaria Afzal Arain, Inzamam Ali Jamali, Afsar Ali Kalhoro
    Journal of Quality Assurance in Agricultural Sciences .2025; 5(1): 12.     CrossRef
  • Drought affects Fe deficiency‐induced responses in a purple durum wheat (Triticum turgidum subsp. durum) genotype
    G. Quagliata, M. D. G. Molina, G. Mannino, E. Coppa, M. N. Saidi, S. Palombieri, F. Sestili, G. Vigani, S. Astolfi
    Plant Biology.2025;[Epub]     CrossRef
  • Rye‐durum wheat 1BL.1RS translocation: implications for drought tolerance and nutritional status
    Giulia Quagliata, Moez Maghrebi, Miriam Marín‐Sanz, Samuela Palombieri, Francesco Sestili, Domenico Lafiandra, Francisco Barro, Gianpiero Vigani, Stefania Astolfi
    Physiologia Plantarum.2024;[Epub]     CrossRef
  • The most relevant drought-tolerant indices for selecting barley drought-tolerant genotypes
    Mbarek Ben Naceur, Hatem Cheikh-mhamed
    Frontiers in Life Sciences and Related Technologies.2024; 5(1): 15.     CrossRef
  • In vitro selection for drought and salt stress tolerance in rice: an overview
    Monika Sahu, Shrinkhla Maurya, Zenu Jha
    Plant Physiology Reports.2023; 28(1): 8.     CrossRef
  • Polyethylene glycol mediated improved shoot proliferation, foliar morpho-anatomy, and rooting of micropropagated shoots of Spathoglottis plicata Blume
    Manokari M, Priyadharshini S, Cokulraj M, Jayaprakash K, Abhijit Dey, Mohammad Faisal, Abdulrahman A. Alatar, Anshu Alok, Mahipal S. Shekhawat
    South African Journal of Botany.2022; 146: 897.     CrossRef
  • Crop Improvement and Abiotic Stress Tolerance Promoted by Moringa Leaf Extract
    Md. Abir Ul Islam, Juthy Abedin Nupur, Charles T. Hunter, Abdullah Al Mamun Sohag, Ashaduzzaman Sagar, Md. Sazzad Hossain, Mona F. A. Dawood, Arafat Abdel Hamed Abdel Latef, Marián Brestič, Md. Tahjib-UI-Arif
    Phyton.2022; 91(8): 1557.     CrossRef
  • PEG-Induced Osmotic Stress Alters Root Morphology and Root Hair Traits in Wheat Genotypes
    Arif Hasan Khan Robin, Shatabdi Ghosh, Md. Abu Shahed
    Plants.2021; 10(6): 1042.     CrossRef
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Characterization of Agronomic Traits and Composition of Antioxidant Compounds in Sweet Sorghum (Sorghum bicolor L. Moench) Germplasms
Jae Il Lyu, Jaihyunk Ryu, Dong-Gun Kim, Jung Min Kim, Min-Kyu Lee, Jin-Baek Kim, Joon-Woo Ahn, Soon-Jae Kwon
Plant Breed. Biotech. 2019;7(2):132-140.   Published online June 1, 2019
DOI: https://doi.org/10.9787/PBB.2019.7.2.132

Sweet sorghum (Sorghum bicolor L. Moench) is one of the most important crops for bioethanol production and the provision of antioxidant compounds for human health. In this study, we investigated the 8 agronomic traits of 153 sweet sorghum germplasms, which demonstrated a variety of phenotypes. In particular, nine sweet sorghum germplasms exhibited a sugar content exceeding 20 Brix. Based on agronomic characteristics, we selected eight elite sweet sorghum germplasms that showed high-performance agronomic and growth characteristics such as tall height, large panicle size with short height, high sugar content, and seed-specific characteristics. The selected germplasms also showed significant differential amount of 3 antioxidant compounds of 3-deoxyanthocyanins, flavonoids, and tannins. SS113 contained the highest levels of total 3-deoxyanthocyanins with apigeninidin contents 3–9 fold higher than that of other germplasms, while SS129 had a white seed coat with the highest recorded total flavonoid level (7.52 mg/g) but no detectable 3-deoxyanthocyanins compounds. The characterization of the traits and compounds will be useful for basic research into the selection of suitable cultivars in the breeding of sweet sorghum.

Citations

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  • Grain yield, nutritional, polyphenols and antioxidant capacity in accessions of sorghum (Sorghum bicolor L. Moench)
    Silvia UÑATE-FRAGA, Josué I. GARCÍA-LÓPEZ, Antonio FLORES-NAVEDA, Norma RUIZ-TORRES, Sonia RAMÍREZ-BARRÓN, Agustín HERNÁNDEZ-JUÁREZ, Alejandro LOZANO-DEL RÍO, Julio C. TAFOLLA-ARELLANO
    Notulae Botanicae Horti Agrobotanici Cluj-Napoca.2022; 50(1): 12637.     CrossRef
  • Comparison of Salinity Tolerance Between Grain and Sweet Sorghum Germplasms [Sorghum Bicolor (L.) Moench]
    Jung Min Kim, Jae Il Lyu, Jaihyunk Ryu, Dong-Gun Kim, Min-Kyu Lee, Jin-Baek Kim, Bo-Keun Ha, Joon-Woo Ahn, Soon-Jae Kwon
    Korean Journal of Breeding Science.2020; 52(1): 32.     CrossRef
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Phytochemical Compounds and Antioxidant Activity in the Grain of Selected Faba Bean (Vicia faba) Genotypes
Soon-Jae Kwon, Dong-Gun Kim, Jung Min Kim, Kyung-Yun Kang, Min-Kyu Lee, Min Jeong Hong, Jin-Baek Kim, Seok Hyun Eom, Si-Yong Kang, Bo-Keun Ha, Jaihyunk Ryu
Plant Breed. Biotech. 2018;6(1):65-73.   Published online March 1, 2018
DOI: https://doi.org/10.9787/PBB.2018.6.1.65

The faba bean produces medicinally useful phenolic compounds naturally, and is also a good source of food and animal feed. This study quantified the functional compounds and antioxidant activities of five faba bean genotypes selected on the basis of their seed yield and L-dopa content. The highest total tannin content (TTC) and total proanthocyanidin content (TPAC) were observed in the PI252004 genotype and the lowest content in the PI366039 genotype. The total phenolics content (TPC) and total flavonoid content (TFC) of the PI430715 genotype were higher than those of the other genotypes. LC-MS analyses identified 22 different phenolic compounds in the faba bean genotypes. The most abundant proanthocyanidin compounds were prodelphinidin and catechin and the predominant flavonols and flavones were myricetin, quercetin, apigenin, kaempferol, and luteolin-free aglycones or glycosides. The TFC was correlated with ferric-reducing antioxidant power activity (FRAP) (P ≤ 0.05), Trolox equivalent antioxidant capacity (TEAC) (P ≤ 0.01), and DPPH radical scavenging activity (P ≤ 0.01). The results indicated that the PI430715 genotype contained the highest TPC and TFC and exhibited the highest FRAP, TEAC and DPPH radical scavenging activities. These results may help determine the optimal genotypes for breeding new cultivars of faba bean with potentially greater health benefits.

Citations

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  • Chemometric Approach to Fatty Acid Composition in Green and Mature Seeds of Selected Faba Bean ( Vicia faba L.) Populations from Serbia
    Violeta Mitic, Jelena Nikolic, Vesna Stankov Jovanovic, Jasmina Milenkovic, Ivana Zlatanovic, Biljana Arsic, Gordana Stojanovic
    Analytical Letters.2026; 59(6): 960.     CrossRef
  • Significant impact of Bacillus licheniformis DW4, Salinicoccus sesuvii DW5 and Paenalcaligenes suwonensis DW7, in mitigation of seawater stress on the growth and productivity of Vicia Faba cultivated in Qalabshu semi-field soil
    Dalia Wael, Yasser El-Amier, WesamEldin I. A. Saber, Ashraf Elsayed
    BMC Plant Biology.2026;[Epub]     CrossRef
  • Structural and phylogenetic insights from complete chloroplast genomes of seven Vicia species
    Mohammad Mehdi Golchini, Aboozar Soorni, Md. Mahmudul Hasan
    PLOS One.2026; 21(3): e0340630.     CrossRef
  • Effects of transglutaminase and thyme oil addition on the properties of faba bean protein-based active films
    Melissa Kocatepe, Kubra Ertan, Serpil Sahin, Gulum Sumnu
    International Journal of Biological Macromolecules.2025; 312: 144178.     CrossRef
  • Unraveling the functional potential of microbial resources and pulse-based matrices for sourdough breadmaking
    Chiara Viretto, Ali Zein Alabiden Tlais, Kashika Arora, Hana Ameur, Fabio Tuccillo, Andrea Polo, Vimac Nolla Ardèvol, Fabienne Verté, Kati Katina, Raffaella Di Cagno, Marco Gobbetti
    Future Foods.2025; 11: 100643.     CrossRef
  • Phytochemical Profiling, Antioxidant Capacity, and α-Amylase/α-Glucosidase Inhibitory Effects of 29 Faba Bean (Vicia faba L.) Varieties from China
    Ying Li, Zhihua Wang, Chengkai Mei, Wenqi Sun, Xingxing Yuan, Jing Wang, Wuyang Huang
    Biology.2025; 14(8): 982.     CrossRef
  • Ethnoknowledge About Vinagreira (roselle) (hibiscus sabdariffa l.) Among residents of Rural Communities in Maranhão, Northeast Brazil
    Osiel César Da Trindade Junior, Edna Maria Ferreira Chaves, Roseli Farias Melo De Barros
    Revista de Gestão Social e Ambiental.2024; 18(3): e04725.     CrossRef
  • Metabolites profiling, in-vitro and molecular docking studies of five legume seeds for Alzheimer’s disease
    Rana M. Ibrahim, Passent M. Abdel-Baki, Osama G. Mohamed, Ahmed A. Al-Karmalawy, Ashootosh Tripathi, Riham A. El-Shiekh
    Scientific Reports.2024;[Epub]     CrossRef
  • Underutilized legumes of India: a potential food resource for the future
    Sani Das, Antara Bose, Muktadhar Reang, Badal Kumar Datta
    Discover Plants.2024;[Epub]     CrossRef
  • Bioaccessibility and potential bioactivity of fresh and mature fava bean flavonoids
    D. Sosa De León, L.F. Méndez-López, B.E. González-Martínez, M. López-Cabanillas Lomelí, A.A. López-Hernández, D.A. Luna-Vital, D. Néder-Suárez, J.A. Vázquez-Rodríguez
    Applied Food Research.2024; 4(2): 100523.     CrossRef
  • Antioxidant Activities, Total Phenols, and Proanthocyanidin Changes during Storage of Fourteen Faba Bean ( Vicia faba L.) Populations from Serbia – A Chemometric Approach
    Violeta Mitic, Jelena Nikolic, Snezana Andjelkovic, Mirjana Petrovic, Vesna Stankov Jovanovic, Jasmina Milenkovic
    Analytical Letters.2024; 57(4): 607.     CrossRef
  • Prediction of antioxidant capacity in faba bean from individual phenolic constituents
    Joel B. Johnson, Anatoliy Kazak, Nadezhda Gallini, Marina Rudenko, Mani Naiker
    Chemical Papers.2024; 78(7): 4285.     CrossRef
  • Effects of Different Processing Methods on Pulses Phytochemicals: An Overview
    Jiajing Zhou, Minhao Li, Qian Bai, Thaiza S. P. de Souza, Colin Barrow, Frank Dunshea, Hafiz A. R. Suleria
    Food Reviews International.2024; 40(4): 1138.     CrossRef
  • Heating effect on nutritional value from two morphotypes (palmate and semi-palmate leaves) of Hibiscus sabdariffa
    Antoine Sambou, Nicolas Ayessou, Aly Diallo
    International Journal of Gastronomy and Food Science.2023; 32: 100698.     CrossRef
  • Nutrient Levels, Bioactive Metabolite Contents, and Antioxidant Capacities of Faba Beans as Affected by Dehulling
    Yu-Mi Choi, Hyemyeong Yoon, Myoung-Jae Shin, Sukyeung Lee, Jungyoon Yi, Young-ah Jeon, Xiaohan Wang, Kebede Taye Desta
    Foods.2023; 12(22): 4063.     CrossRef
  • Atmospheric CO2 concentration affects the life cycle, yield, and fruit quality of early maturing edible legume cultivars
    Idoia Garmendia, Sakineh Rashidi, Marilyn RA Quezada‐Salirrosas, Nieves Goicoechea
    Journal of the Science of Food and Agriculture.2022; 102(10): 3964.     CrossRef
  • Vicia plants—A comprehensive review on chemical composition and phytopharmacology
    Bahare Salehi, Ibrahim M. Abu‐Reidah, Farukh Sharopov, Natallia Karazhan, Javad Sharifi‐Rad, Muhammad Akram, Muhammad Daniyal, Fahad Said Khan, Wafa Abbaass, Rida Zainab, Katya Carbone, Nouran M. Fahmy, Eman Al‐Sayed, Mohamed El‐Shazly, Massimo Lucarini,
    Phytotherapy Research.2021; 35(2): 790.     CrossRef
  • L-3,4-dihydroxyphenylalanine Accumulation in Faba Bean (Vicia faba L.) Tissues during Different Growth Stages
    Shucheng Duan, Soon Jae Kwon, You Jin Lim, Chan Saem Gil, Chengwu Jin, Seok Hyun Eom
    Agronomy.2021; 11(3): 502.     CrossRef
  • Impact of gamma radiation on antioxidant activity in faba bean (Vicia faba L.) and the potential of meatballs formulation with inclusion of the powder of irradiated beans
    Aly H. Rady, Abbas O. Toliba, Hesham M. Badr, Amr Kh. Ali
    Journal of Food Science and Technology.2020; 57(8): 2975.     CrossRef
  • Phenolic Compound Content of Leaf Extracts from Different Roselle (Hibiscus sabdariffa) Accessions
    Jae Il Lyu, Jung Min Kim, Dong-Gun Kim, Jin-Baek Kim, Sang Hoon Kim, Joon-Woo Ahn, Si-Yong Kang, Jaihyunk Ryu, Soon-Jae Kwon
    Plant Breeding and Biotechnology.2020; 8(1): 1.     CrossRef
  • In vitro investigation of hypoglycemic and oxidative stress properties of fava bean (Vicia faba L.) seed extract in Saccharomyces cerevisiae 2376
    Dhiraj Kumar Choudhary, Abha Mishra
    Preparative Biochemistry & Biotechnology.2018; 48(10): 920.     CrossRef
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Compositional Analysis of Lentil (Lens culinaris) Cultivars Related to Colors and Their Antioxidative Activity
So-Young Lee, Yun-Soo Yeo, Soo-Yun Park, Seong-Gon Lee, Si-Myung Lee, Hyun-Suk Cho, Nam-Jin Chung, Seon-Woo Oh
Plant Breed. Biotech. 2017;5(3):192-203.   Published online September 1, 2017
DOI: https://doi.org/10.9787/PBB.2017.5.3.192

Metabolite profile is a powerful analytical technique to identify the functional characterization of plants. In this study, the phytochemicals and secondary metabolites of lentils (Lens culinaris) were analyzed to compare the anti-oxidative activities according to the different colors. The polar metabolites, fatty acids, carotenoids, flavonoids, anthocyanins, total phenolic acids, DPPH activity were analyzed. Three kind of lentils, French green whole lentil (FG), red whole lentil (LR), and green whole lentil (LG) (ASIA SEED Co., LTD), were used for this study. Fatty acids, phytochemicals, and antioxidative components from each lentil varieties were analyzed by official methods. The contents of lutein in carotenoids were 6–9 times higher than zeaxanthin in all lentils, but were not significantly different among three varieties. The content of carotenoids in FG was lower significantly than those in the LR and LG. Myricetin and luteolin were detected in the only FG. Kaempferol and delphinidin were significantly highest in the FG. Most of the phenolic acids except coumarate were higher in FG and LG than in LR. Also antioxidant effects (EC50) were higher in FG and LG than in LR. The analyzed metabolites obtained from lentils showed distinct separation in the PCA results according to the varieties. Also, lentils showed different anti-oxidant profiles according to the colors. FG and LG showing higher contents of phytochemicals showed higher antioxidative activity than LG containing relative low contents of phytochemicals.

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  • One-pot green solution combustion synthesis of ZnV2O6 photo catalyst for dye degradation, Cr (VI) detoxification, phyto toxicity analysis and latent finger printing applications
    R Nalina, Udayabhanu, R. Harini, K.P. Latha, K.B. Naveen, Mohd Shkir, H.N. Shwetha, K.N. Nandeesh, G. Nagaraju
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    International Journal of Environmental Analytical Chemistry.2024; 104(19): 7855.     CrossRef
  • Lactic and propionic acid bacteria starter cultures for improved nutritional properties of pea, faba bean and lentil
    Minna Kahala, Lucia Blasco, Rina Bragge, Davide Porcellato, Hilde Marit Østlie, Thomas Rundberget, Jose Antonio Baz-Lomba, Juha-Matti Pihlava, Jarkko Hellström, Emilie Gullberg Jørgensen, Vesa Joutsjoki, Tove Gulbrandsen Devold, Anne Pihlanto
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  • Phenolic profile of whole seeds and seed fractions of lentils and its impact on antioxidant activity
    Antonio Manco, Carmela Gerardi, Giuseppe Romano, Leone D'Amico, Antonio Blanco, Francesco Milano, Gian Pietro Di Sansebastiano, Rind Balech, Barbara Laddomada
    Food Bioscience.2023; 54: 102887.     CrossRef
  • Tissue-Specific Antioxidant Activities of Germinated Seeds in Lentil Cultivars during Thermal Processing
    Ji Hye Kim, Shucheng Duan, You Rang Park, Seok Hyun Eom
    Antioxidants.2023; 12(3): 670.     CrossRef
  • Lentil (Lens culinaris Medik.) Flour Varieties as Promising New Ingredients for Gluten-Free Cookies
    Lívia Hajas, László Sipos, Éva Csajbókné Csobod, Márta Veresné Bálint, Réka Juhász, Csilla Benedek
    Foods.2022; 11(14): 2028.     CrossRef
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    Theresa Boeck, Lilit Ispiryan, Andrea Hoehnel, Aylin W. Sahin, Aidan Coffey, Emanuele Zannini, Elke K. Arendt
    Foods.2022; 11(14): 2013.     CrossRef
  • Protective effects of functional foods against Parkinson's disease: A narrative review on pharmacology, phytochemistry, and molecular mechanisms
    Milad Iranshahy, Behjat Javadi, Amirhossein Sahebkar
    Phytotherapy Research.2022; 36(5): 1952.     CrossRef
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Chemical Constitutions and Antioxidant Activities of Tomato Leaf Extracts
Kyung Jun Lee, Gi-An Lee, Kyung-Ho Ma, Sebastin Raveendar, Yang-Hee Cho, Jung-Ro Lee, Jong-Wook Chung
Plant Breed. Biotech. 2016;4(3):362-372.   Published online August 31, 2016
DOI: https://doi.org/10.9787/PBB.2016.4.3.362

The present study aimed to determine the contents of five flavonols and two glycoalkaloids as well as the antioxidant activities of leaf ethanol extracts of 50 tomato accessions. The antioxidant activity was assessed using different tests: 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), 2,2-diphenyl-1-picryl hydrazyl (DPPH), nitric oxide (NO), and total polyphenol content (TPC). Flavonols and glycoalkaloids contents were determined using a high performance liquid chromatography-diode array detector system. Among five flavonols and two glycoalkaloids, naringenin and tomatine were detected in tomato leaves at high concentrations. Of the 50 tomato accessions, IT 229711, IT2365203, and IT 207224 were found to have the highest contents of quercetin, kaempferol, and tomatine, respectively. Leaf extract of IT189949 exhibited the highest relative antioxidant capacity index (RACI). Among the five flavonols, myricetin showed positive correlations with DPPH, ABTS, and NO, while isorhamnetin had positive correlation with DPPH. These results will expand the chemical constitution database and provide information on tomato leaves. They are valuable for the development of functional foods or feed-additives.

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Plant Breed. Biotech. 2016;4(2):242-251.   Published online May 31, 2016
DOI: https://doi.org/10.9787/PBB.2016.4.2.242

Blackberries (Rubus fruticosus L.) are a good source of antioxidants and contain appreciable levels of functional compounds. This study was carried out to evaluate fruit qualities (pH, soluble solids content, titratable acidity, and minerals), functional compounds (total phenolic content, total flavonoid content, anthocyanins, and ellagic acid), and antioxidant activity among five mutated blackberry lines over three ripening stages to examine the effect of fruit maturation on functional compounds and antioxidant activity. The content of sugars, total phenolic content, total flavonoid content, and anthocyanins (cyanidin-3-O-glucoside, cyanidin-3-O-xyloside, cyanidin-3-O-malonylglucoside, and cyanidin-3-O-dioxalylglucoside) increased as fruit development advanced, whereas the ellagic acid content decreased as the fruit became more mature. Among the minerals tested, potassium showed the highest concentrations at all stages of development. The antioxidant activity was correlated with total phenolic content (P≤0.01), total flavonoid content (P≤0.01), and total anthocyanins (P≤0.05). The results indicate that Maple and B201 lines contained the highest anthocyanin content and exhibited greater antioxidant activity.

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Assessment of the Response of Beta Carotene Enhanced Transgenic Soybeans to Soybean Mosaic Virus (SMV)
Yang Qin, Kong-Sik Shin, Jang-Kyun Seo, Myung-Ho Lim, Young-Soo Chung, Hyun-Suk Cho, Seong-Kon Lee, Hee-Jong Woo
Plant Breed. Biotech. 2016;4(2):158-169.   Published online May 31, 2016
DOI: https://doi.org/10.9787/PBB.2016.4.2.158

Beta-carotene, a defense chemical, is synthesized by the carotenoid biosynthesis pathway. In the present study, a transgenic soybean line, with a single copy insertion of phytoene synthase and carotene desaturase genes, having high beta-carotene content was studied for its response to systemically inoculated Soybean mosaic virus (SMV). Beta-carotene-enhanced transgenic soybean showed similar leaf and seed symptoms, viral RNA, and protein expression compared to the non-genetically modified (GM) ‘Kwangan’ control. Total antioxidant contents in the non-GM ‘Kwangan’ line were increased after SMV attack in both leaves and seeds; however, the antioxidant contents in the beta-carotene-enhanced soybean line have no significant changes. In addition, both GM and non-GM soybean were detected increased lipid hydroperoxide concentrations in leaves and seeds after SMV infection, even though they did not reach a statistical significant level. Abscisic acid (ABA) levels in beta-carotene-enhanced transgenic soybean seeds was determined 35-fold increase after SMV infections caused a lower seed germination rate and a higher SMV transmission rate to subsequent generations, compared to those of non-GM ‘Kwangan’. Thus, we concluded that the additional production of beta-carotene did not confer resistance of beta-carotene-enhanced transgenic soybean to SMV infections, but caused mass accumulations of ABA in seeds.

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    Applied Biological Chemistry.2017; 60(3): 299.     CrossRef
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Variation in Antioxidant Activity and Flavonoid Aglycones in Eggplant (Solanum melongena L.) Germplasm
Xiang-Min Piao, Jong-Wook Chung, Gi-An Lee, Jung-Ro Lee, Gyu-Taek Cho, Ho-Sun Lee, Kyung-Ho Ma, Jing Guo, Hong Sig Kim, Sok-Young Lee
Plant Breed. Biotech. 2014;2(4):396-403.   Published online December 31, 2014
DOI: https://doi.org/10.9787/PBB.2014.2.4.396

Eggplant (Solanum melongena L.) is an excellent source of vitamins A and C and of flavonoid compounds, which are important antioxidant components believed to reduce the risk of various diseases. We investigated the antioxidant activity and flavonoid content in eggplant leaves and fruits to identify genetic resources with high antioxidant capacity for use in food or as feed additives, and also determined the influence of days to flowering, leaf blade colors, and latitudes of origin on the antioxidant activity and flavonoid content in eggplant leaves. The accessions originating from 45°N showed the highest flavonoid contents (AVG. = 15.4 μg mg−1) followed by accessions from 30°~45°N (AVG. = 13.0 μg mg−1), 15°~30°N (AVG. = 11.0 μg mg−1), and 0°~15°N (AVG. = 9.5 μg mg−1). The same pattern was also found in 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 1,1-diphenyl-2-picryl-hydrazil (DPPH) antioxidant activities. High ABTS and DPPH activity and flavonoid content were found in the early-flowering accessions. All flavonoids of the greenish violet leaves were significantly higher than those of green leaves. The flavonoid concentration in eggplant leaves was 10 to 20 fold greater, at an average of 15.6 μg mg−1, than that of the fruit (AVG. = 0.9 μg mg−1). Taken together, eggplant leaves represent a potential source of natural antioxidants due to their high flavonoid content.

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    Sang Suk Kim, YoSup Park, Seung-Gab Han
    Korean Journal of Breeding Science.2023; 55(3): 244.     CrossRef
  • Metabolomic Analysis of Phytochemical Compounds from Agricultural Residues of Eggplant (Solanum melongena L.)
    Laura Aracely Contreras-Angulo, Aldo Moreno-Ulloa, Rommel A. Carballo-Castañeda, Josefina León-Felix, José Geovanni Romero-Quintana, Maribel Aguilar-Medina, Rosalío Ramos-Payán, J. Basilio Heredia
    Molecules.2022; 27(20): 7013.     CrossRef
  • Assessing Physicochemical Parameters, Bioactive Profile and Antioxidant Status of Different Fruit Parts of Greek Eggplant Germplasm
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    Horticulturae.2022; 8(12): 1113.     CrossRef
  • Biochemical Composition of Eggplant Fruits: A Review
    Meenakshi Sharma, Prashant Kaushik
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  • A Polyphenol Rich Extract from Solanum melongena L. DR2 Peel Exhibits Antioxidant Properties and Anti-Herpes Simplex Virus Type 1 Activity In Vitro
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    Molecules.2018; 23(8): 2066.     CrossRef
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Variation in Antioxidant Activity and Polyphenol Content in Tomato Stems and Leaves
Xiang-Min Piao, Eun-Kyu Jang, Jong-Wook Chung, Gi-An Lee, Ho-Sun Lee, Jung-Sook Sung, Young-Ah Jeon, Jung-Ro Lee, Yeon-Gyu Kim, Sok-Young Lee
Plant Breed. Biotech. 2013;1(4):366-373.   Published online December 31, 2013
DOI: https://doi.org/10.9787/PBB.2013.1.4.366

Tomato was considered as one of the most widely cultivated vegetable crops in the world. Tomato plant has high antioxidant capacity which can be attributed to the high levels of carotenoids, phenols, vitamins C and E. However, most of tomato plants have been discarded as waste after fruit harvesting. In order to identify genetic resources with high antioxidant level for use in food or as feed additives, we investigated the ABTS, DPPH antioxidant activity and polyphenol content in tomato leaves and stems. A total of 112 tomato accessions were classified into three groups by latitude of their collected countries: 30°~60° North (50 accessions), 0°~30° North (40 accessions), and 0°~30° South (22 accessions). Stem and leaf extracts showed wide variation in ABTS antioxidant activity ranging from 1.6 ± 1.0 to 48.4 ± 6.1 μg Trolox mg−1 dw. The antioxidant activity of DPPH was in the range of 6.3 ± 0.2 to 40.0 ± 0.3 μg ASC mg−1 dw. Total polyphenol content ranged from 6.1 ± 0.2 to 38.9 ± 0.7 μg GAE mg−1 dw. ABTS, DPPH antioxidant activities and polyphenol content in accessions from 30°~60°N latitude were significantly higher (P<0.05) than those from 0°~30°N latitude. ABTS values showed a significant positive correlation (r = 0.700**) with DPPH activity. IT100506 (KOR) and 702959 (UKR) were recommended as potential sources of natural antioxidants due to their highest antioxidant activity among accessions. This study will provide valuable information for tomato breeders in developing and producing functional food or feed additives resources.

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