Confirmation of T-DNA inheritance and stability requires testing of several parameters such as phenotypic performance of transgenic lines, transgene integrity structure and expression stability for at least three consecutive generations, and Mendelian inheritance analysis, which are very important to transgenic breeding and commercialization of genetically modified crops (GMOs). In the present study, we measured nine agronomic and yield-related traits of Iksan526 and confirmed stable phenotypic performance comparing to the donor variety Dongjin over three years. Transgene integrity structure, flanking sequence, functional element copy numbers, absence of backbone sequence and transgene expression were all verified in Iksan526 over three consecutive generations, indicating a stable inheritance of the transgene. In addition, phenotypic and genotypic segregation were analyzed on F₂ and F₃ populations derived from a cross of Dongjin/Iksan526, and results complied with Mendel’s law of segregation based on single transgene locus. This study demonstrated detailed analyses and confirmation of transgene event Iksan526 which is crucial for commercialization of GMO.

Citations

Citations to this article as recorded by  

• Analysis of T-DNA integration events in transgenic rice
  Wankui Gong, Yun Zhou, Rui Wang, Xiaoli Wei, Lei Zhang, Yan Dai, Zhen Zhu
  Journal of Plant Physiology.2021; 266: 153527.     CrossRef
• Genetic Stability, Inheritance Patterns and Expression Stability in Biotech Crops
  Laura Privalle, Patricia Back, Apurv Bhargava, Zach Bishop, Krystal Cisneros, Isabelle Coats, Ine Criel, Lien Dhondt, Travis Draughn, Barb Fowler, Brad Franklin, Durba Ghoshal, Jim Lor, Jennifer Massengil, Sofie Moens, Tyson Mooney, Dannyel Nelson, Karoli
  OBM Genetics.2020; 04(04): 1.     CrossRef