Powdery mildew (PM), caused by the biotrophic fungus Podosphaera xanthii, drastically reduces the yield and quality of melon (Cucumis melo L.). Knowledge of the genetic control and high throughput molecular markers linked with resistance against this disease are essential for breeding programs. The bioassay study of the F1 and F2 populations derived from the parents, ‘PMR 5’ (♂) and ‘SCNU1154’ (♀) revealed a monogenic dominant nature of resistance to the devastating race, race 5. Besides, we developed three SNP based high resolution melting markers, PMm-HRM-1, PMm-HRM-2, and PMm-HRM-3, based on the previously identified SNPs on chromosome 12 and validated them using 8 melon lines and 137 F2 populations. Among these, the SNP of marker PMm-HRM-1 causes a missense mutation in the LRR region of MELO3C002393 and we were able to distinguish the resistant vs susceptible genotypes from eight diverse melon accessions and the segregating F2 population with more than 90% genotyping efficiency. The other two markers were based on intergenic SNPs and had more than 80% genotyping efficiency in F2 population. These markers will be helpful to melon breeders to develop melon cultivars resistant to P. xanthii race 5 via marker assisted breeding programs.

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• Developing DNA Markers based on Male-Specific Chromosomal Regions for Selecting Male Plants in Hop (Humulus lupulus)
  Tae hyun Ha, Jae Il Lyu, So Young Yi, Si-Yong Kang
  Plant Breeding and Biotechnology.2024;[Epub]     CrossRef
• Identification of Gene Responsible for Conferring Resistance against Race KN2 of Podosphaera xanthii in Melon
  Sopheak Kheng, San-Ha Choe, Nihar Sahu, Jong-In Park, Hoy-Taek Kim
  International Journal of Molecular Sciences.2024; 25(2): 1134.     CrossRef

During seed production of onion (Allium cepa L.) and Welsh onion (A. fistulosum L.) cultivars, seeds are inadvertently cross-contaminated with each other. However, it is difficult to identify cross-contaminated seeds by visual examination since seed and seedling morphologies of onion and Welsh onion are almost identical. To develop molecular markers for distinguishing onion and Welsh onion at early seedling stages, polymorphic mitochondrial genome sequences between two species were isolated. Using complete mitochondrial genome sequences of onions as references, genome walking was performed to isolate polymorphic Welsh onion sequences. Unlike conserved 3′ sequences flanking the atp9 gene, the 5′ flanking sequences were completely different between onion and Welsh onion mitochondrial genomes. A simple PCR marker was developed on the basis of polymorphic 5′ flanking regions of atp9, and a high resolution melting (HRM) marker was developed based on one of single nucleotide polymorphisms (SNPs) in the 3′ flanking regions. A total of 41 onion and 19 Welsh onion cultivars were analyzed using these two molecular markers. Results showed that the onion-specific marker genotype was detected only in onion cultivars, and vice versa. To estimate distribution of onion-specific and Welsh onion-specific organizations of atp9 among Allium species, 14 Allium species related to onion and Welsh onion were analyzed. Results showed that specific organizations were conserved among closely related species of onion and Welsh onion, respectively, implying that there might be no intraspecific variation in the atp9 organizations.

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• The complex and dynamic mitochondrial genome of garlic (Allium sativum): Insights from structural and evolutionary analysis
  Hui Shen, Wen Liu, Lilian Zhao, Yanfang Guo, Yuanheng Li, Ting Wu, Shu Han
  Genomics.2026; 118(2): 111214.     CrossRef
• Allelic variability and transferability of atp6 gene among Allium species
  Talamarla Yeswanth Mahidar Gowd, Chandra Deo, Dalasanuru Chandregowda Manjunathagowda, Vijay Mahajan, Nangsol Dolma Bhutia, Barun Singh
  Genetic Resources and Crop Evolution.2023; 70(1): 281.     CrossRef
• Mitochondrial relationships between various chamomile accessions
  Joana Ruzicka, Marion Hacek, Johannes Novak
  Journal of Applied Genetics.2021; 62(1): 73.     CrossRef