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"Hye Jung Lee"

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"Hye Jung Lee"

Research Articles

Enhancement of Rice Resistance to Bacterial Blight by Overexpressing BrCP3 Gene of Brassica rapa
Marjohn C. Niño, Hye Jung Lee, Joonki Kim, Sailila E. Abdula, Yu-Jin Jung, Kwon-Kyoo Kang, Illsup Nou, Yong-Gu Cho
Plant Breed. Biotech. 2015;3(4):355-365.   Published online November 30, 2015
DOI: https://doi.org/10.9787/PBB.2015.3.4.355

Brassica rapa cysteine protease 3 (BrCP3) is an endopeptidase member of C1A family (papain-like) and CA clan of cysteine protease. Members of papain-like cysteine protease (CP) have emerged to be key enzymes involved in cell death in response to biotic and environmental stresses. Recent progress of CP research features its diverse and overlapping role in basal and effector-triggered immunity. This study was carried out to characterize BrCP3 gene in Chinese cabbage and explore its function in rice – Xanthomonas oryzae pv. oryzae pathosystem. Phylogenetic analysis and BLASTP search of its amino acid sequence to the Arabidopsis Protein TAIR 10 database identified BrCP3 as the closest ortholog of Arabidopsis RD21a. The full-length cDNA of BrCP3 was cloned and transformed into the genome of japonica rice cv. ‘Gopum’. Constitutive overexpression of the transgene is driven by Ubi-1 promoter in pFLCIII vector. Regenerated single copy overexpression rice lines were inoculated with three Xoo Korean isolates. Regulation of rice resistance through cysteine protease activity is evident in the overexpression lines which showed enhanced immunity to three isolates as depicted by reduced lesion length compared with the wild type. Our initial results implicate an interesting biological function of BrCP3 in rice system that has never been reported before.

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  • Development of Transgenic Brassica Crops against Biotic Stresses Caused by Pathogens and Arthropod Pests
    Jorge Poveda, Marta Francisco, M. Elena Cartea, Pablo Velasco
    Plants.2020; 9(12): 1664.     CrossRef
  • Overexpression of rice premnaspirodiene oxygenase reduces the infection rate ofXanthomonas oryzaepv.oryzae
    Marjohn C. Niño, Jae-Young Song, Franz Marielle Nogoy, Me-Sun Kim, Yu Jin Jung, Kwon-Kyoo Kang, Illsup Nou, Yong-Gu Cho
    Journal of Plant Biotechnology.2016; 43(4): 422.     CrossRef
  • Application and utilization of marker assisted selection for biotic stress resistance in hybrid rice (Oryza sativaL.)
    Jae-Young Song, Sothea Ouk, Franz Marielle Nogoy, Marjohn C. Niño, Soon Wook Kwon, Woongoo Ha, Kwon-Kyoo Kang, Yong-Gu Cho
    Journal of Plant Biotechnology.2016; 43(3): 317.     CrossRef
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Modification of Starch Composition Using RNAi Targeting Soluble Starch Synthase I in Japonica Rice
Hye Jung Lee, Moo-Geun Jee, Joonki Kim, Franz M.C. Nogoy, Marjohn C. Niño, Dal-A Yu, Me Sun Kim, Mingmao Sun, Kwon-Kyoo Kang, Illsup Nou, Yong-Gu Cho
Plant Breed. Biotech. 2014;2(3):301-312.   Published online September 30, 2014
DOI: https://doi.org/10.9787/PBB.2014.2.3.301

An increasing preference for good eating quality of rice among consumers has become one of the important considerations in rice breeding. Amylose content is a leading factor affecting eating quality of rice. Amylose composition is determined by the relative activity of soluble starch synthase (SSS) and granule-bound starch synthase (GBSS). This study focused on modifying the expression of SSSI gene which is responsible for amylopectin and amylose synthesis in rice by using RNA interference (RNAi) technology. The transgenic rice plants showed various amylose contents (11–17%) in rice grains. Favorable rice lines were selected according to genomic PCR, transgene expression and amylose contents analysis. A semi-quantitative RT-PCR was carried out to determine the expression level of SSSI gene after flowering of transgenic rice and wild type. Down-regulation of SSSI gene in transgenic plants was evident in the decreasing expression in rice grains. Accordingly, scanning electron microscopy (SEM) analysis revealed uniform size with smooth curves starch granules in down-regulation rice lines, in contrast with the non-uniform granules in wild type. Results indicated that RNAi-SSSI transgenic lines produced low amylose contents that fell between glutinous and non-glutinous rice. This study showed that down-regulation of endogenous SSSI may improve the eating quality in rice.

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  • Biochemical markers for low glycemic index and approaches to alter starch digestibility in rice
    Awadhesh Kumar, Upasana Sahoo, Milan Kumar Lal, Rahul Kumar Tiwari, Sangram Keshari Lenka, Nihar Ranjan Singh, Om Prakash Gupta, Rameswar Prasad Sah, Srigopal Sharma
    Journal of Cereal Science.2022; 106: 103501.     CrossRef
  • Soluble Starch Synthase Enzymes in Cereals: An Updated Review
    Ahsan Irshad, Huijun Guo, Shoaib Ur Rehman, Xueqing Wang, Chaojie Wang, Ali Raza, Chunyun Zhou, Yuting Li, Luxiang Liu
    Agronomy.2021; 11(10): 1983.     CrossRef
  • SSIIIa-RNAi suppression associated changes in rice grain quality and starch biosynthesis metabolism in response to high temperature
    Qian Zhao, Yu Ye, Zhanyu Han, Lujian Zhou, Xianyue Guan, Gang Pan, Muhammad-Asad-Ullah Asad, Fangmin Cheng
    Plant Science.2020; 294: 110443.     CrossRef
  • Physico-chemical characterization and transcriptome analysis of 5-methyltryptophan resistant lines in rice
    Franz Marielle Nogoy, Yu Jin Jung, Kwon-Kyoo Kang, Yong-Gu Cho, Wujun Ma
    PLOS ONE.2019; 14(9): e0222262.     CrossRef
  • Characterization of ‘GolSam’ Lines Developed from the Cross between Samgwang and 5MT Resistant Lines in Rice
    Franz Marielle Nogoy, Yu Jin Jung, Kwon Kyoo Kang, Yong-Gu Cho
    Plant Breeding and Biotechnology.2018; 6(3): 233.     CrossRef
  • Crop resistant starch and genetic improvement: a review of recent advances
    Jian Xia, Dong Zhu, Ruomei Wang, Yue Cui, Yueming Yan
    Theoretical and Applied Genetics.2018; 131(12): 2495.     CrossRef
  • Application of ZFN for Site Directed Mutagenesis of Rice SSIVa Gene
    Yu-Jin Jung, Franz Marielle Nogoy, Sang-Kyu Lee, Yong-Gu Cho, Kwon-Kyoo Kang
    Biotechnology and Bioprocess Engineering.2018; 23(1): 108.     CrossRef
  • Potential impact of genome editing in world agriculture
    Wendy Harwood, Jorge Martínez-Fortún, Dylan W. Phillips, Huw D. Jones
    Emerging Topics in Life Sciences.2017; 1(2): 117.     CrossRef
  • Overexpression of BrCIPK1 Gene Enhances Abiotic Stress Tolerance by Increasing Proline Biosynthesis in Rice
    Sailila E. Abdula, Hye-Jung Lee, Hojin Ryu, Kwon Kyoo Kang, Illsup Nou, Mark E. Sorrells, Yong-Gu Cho
    Plant Molecular Biology Reporter.2016; 34(2): 501.     CrossRef
  • BrUGE1 transgenic rice showed improved growth performance with enhanced drought tolerance
    Sailila E. Abdula, Hye Jung Lee, Joonki Kim, Marjohn C. Niño, Yu-Jin Jung, Young-Chan Cho, Illsup Nou, Kwon-Kyoo Kang, Yong-Gu Cho
    Breeding Science.2016; 66(2): 226.     CrossRef
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Review Article

Key Roles of Cysteine Protease in Different Plant Pathosystem
Marjohn Niño, Joonki Kim, Hye Jung Lee, Sailila E. Abdula, Ill Sup Nou, Yong-Gu Cho
Plant Breed. Biotech. 2014;2(2):97-109.   Published online June 30, 2014
DOI: https://doi.org/10.9787/PBB.2014.2.2.097

Cysteine protease is one of the well-studied proteolytic enzymes in plants. This class of protease has been implicated in various physiological aspects of developmental stages in plants including seed germination, senescence, and disease immunity. A handful of studies assign plants cysteine protease in different molecular battlefield under a few selected pathosystems, and initially extricate complex molecular mechanism of resistance. Interestingly, the same type of proteases released by pathogens have been demonstrated to play a crucial role in establishing disease infection in plants. Most of which target resistance proteins resulting either in an access to the hosts biochemical loot or cascades of signals for defense. As means of enabling and disabling host immunity, this class of protease is subject to diversifying selection, which is an intriguing outcome under natural host pathosystem. This paper summarizes the key roles of cysteine proteases in a few selected plant pathosystems.

Citations

Citations to this article as recorded by  
  • Transcriptome analysis of cacao reveals differentially expressed genes associated with resistance to Phytophthora palmivora
    Nur Kholilatul Izzah, Murukarthick Jayakodi, Sang-Choon Lee, Khaerati, Cici Tresniawati, Widi Amaria, Faizal Maulana, Jee Young Park, Tae-Jin Yang
    3 Biotech.2026;[Epub]     CrossRef
  • Phapa-4574965: A multifunctional effector of Phakopsora pachyrhizi targeting multiple host metabolic components
    Alice Satiko Utiyama, Thays Vieira Bueno, Valeria Yukari Abe, Adriana Brombini dos Santos, Luana M. Darben, Aluízio Borém de Oliveira, Ricardo V. Abdelnoor, Steven A. Whitham, Luciano G. Fietto, Francismar C. Marcelino-Guimarães
    Physiological and Molecular Plant Pathology.2026; 144: 103271.     CrossRef
  • Identification of a Papain-like Cysteine Protease Functioning as an Avirulence Factor in Striga–Cowpea Interactions
    Danhua Zhang, Michael P. Timko
    Plants.2025; 14(10): 1427.     CrossRef
  • Exploring Bioactive Metabolites From Fusarium falciforme and Aspergillus terreus Isolated From Protease‐Rich Fruits: Antifungal, Antitrypanosomal, and Enzymatic Inhibitory Activities
    Gabriela de Oliveira Almeida, Vitor de Souza Mazucato, Ludmilla Tonani, Marcia Regina von Zeska Kress, Gisele Barbosa, Renata Krogh, Adriano Defini Andricopulo, Leonardo Luiz Gomes Ferreira, Paulo Cezar Vieira
    Chemistry & Biodiversity.2025;[Epub]     CrossRef
  • Comprehensive Analysis of C48 Domain Containing Cysteine Protease Enzymes Revealed Their Role During Abiotic Stress and Reproductive Development in Rice
    Rajeev Ranjan, Reema Mishra
    Journal of Plant Growth Regulation.2024; 43(7): 2314.     CrossRef
  • New Genomic Regions Identified for Resistance to Spot Blotch and Terminal Heat Stress in an Interspecific Population of Triticum aestivum and T. spelta
    Sudhir Navathe, Ajeet Kumar Pandey, Sandeep Sharma, Ramesh Chand, Vinod Kumar Mishra, Dinesh Kumar, Sarika Jaiswal, Mir Asif Iquebal, Velu Govindan, Arun Kumar Joshi, Pawan Kumar Singh
    Plants.2022; 11(21): 2987.     CrossRef
  • Efficacy of seed defense proteins in biofortified pearl millet lines against blast and downy mildew
    Marri Swathi, Nimmala Naresh, Tirupaati Swaroopa Rani, Mahalingam Govindaraj, Rajan Sharma
    Acta Physiologiae Plantarum.2021;[Epub]     CrossRef
  • Genome-wide transcriptional response of papain-like cysteine protease-mediated resistance against Xanthomonas oryzae pv. oryzae in rice
    Marjohn C. Niño, Kwon Kyoo Kang, Yong-Gu Cho
    Plant Cell Reports.2020; 39(4): 457.     CrossRef
  • Genome-wide identification and molecular characterization of cysteine protease genes in rice
    Marjohn C. Niño, Me-Sun Kim, Kwon Kyoo Kang, Yong-Gu Cho
    Plant Biotechnology Reports.2020; 14(1): 69.     CrossRef
  • Two Phytophthora parasitica cysteine protease genes, PpCys44 and PpCys45, trigger cell death in various Nicotiana spp. and act as virulence factors
    Qiang Zhang, Weiwei Li, Jiapeng Yang, Junjie Xu, Yuling Meng, Weixing Shan
    Molecular Plant Pathology.2020; 21(4): 541.     CrossRef
  • Involvement of a vascular hypersensitive response in quantitative resistance to Ralstonia solanacearum on tomato rootstock cultivar LS‐89
    K. Nakaho, S. Seo, K. Ookawa, Y. Inoue, S. Ando, Y. Kanayama, S. Miyashita, H. Takahashi
    Plant Pathology.2017; 66(1): 150.     CrossRef
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Research Article
Development and Identification of Transgenic Rice Lines with Abiotic Stress Tolerance by using a Full-length Overexpressor Gene Hunting System
Sailila E. Abdula, Hye Jung Lee, Moo Geun Jee, Yu Jin Jung, Kwon Kyoo Kang, Ill Sup Nou, Sang-Bok Lee, Won-Ha Yang, Yong-Gu Cho
Plant Breed. Biotech. 2013;1(1):33-48.   Published online March 31, 2013
DOI: https://doi.org/10.9787/PBB.2013.1.1.033

The latest report on the draft genome of Brassica rapa sequence has been published. To elucidate the functions of these genes and to efficiently search for agriculturally useful genes, a Full-length cDNA Over-eXpressor (FOX) gene hunting system was used. The FOX library from Chinese cabbage was introduced into rice via Agrobacterium-mediated transformation. Approximately 1,150 FOX-rice lines were generated. Genomic PCR analysis indicated that the average length of FL-cDNAs introduced into individual lines was 900~1,200 bp. Basic Local Alignment System Tool (BLAST) analysis of the FL-cDNA genes revealed that 35.5% have unknown function. Most of the randomly selected transgenic rice lines showed overexpression (92%) of these genes relative to the wild-type Gopum. Moreover, 94% of the 850 transgenic rice lines were moderately tolerant (slightly yellow) to cold and 9 lines were tolerant (seedlings were light green). Morphological evaluation of the transgenic rice lines showed minimal phenotypic alteration (12%). Approximately 25.1% and 22% of the plants were significantly ahead in the days to heading and had elevated chlorophyll content, respectively. Other agronomic traits such as filled grains, number of tiller, panicle length, and culm and plant height were relatively less variable among the transgenic lines. These results provide a resource for defining genes that are associated with tolerance in transgenic rice lines.

Citations

Citations to this article as recorded by  
  • A systematic review of the Uridine diphosphate-Galactose/Glucose-4-epimerase (UGE) in plants
    Jiaming Hou, Shaokai Tian, Lin Yang, Zhixin Zhang, Ying Liu
    Plant Growth Regulation.2021; 93(3): 267.     CrossRef
  • Gene expression and SNP identification related to leaf angle traits using a genome-wide association study in rice (Oryza sativaL.)
    Me-Sun Kim, Yeisoo Yu, Kwon-Kyoo Kang, Yong-Gu Cho
    Journal of Plant Biotechnology.2018; 45(1): 17.     CrossRef
  • SP-LL-37, human antimicrobial peptide, enhances disease resistance in transgenic rice
    In Hye Lee, Yu-Jin Jung, Yong Gu Cho, Ill Sup Nou, Md. Amdadul Huq, Franz Marielle Nogoy, Kwon-Kyoo Kang, Zonghua Wang
    PLOS ONE.2017; 12(3): e0172936.     CrossRef
  • Brassica rapaSec14-like protein geneBrPATL4determines the genetic architecture of seed size and shape
    Joonki Kim, Hye-Jung Lee, Franz Marielle Nogoy, Dal-A Yu, Me-Sun Kim, Kwon-Kyoo Kang, Illsup Nou, Yong-Gu Cho
    Journal of Plant Biotechnology.2016; 43(3): 332.     CrossRef
  • Overexpression of BrCIPK1 Gene Enhances Abiotic Stress Tolerance by Increasing Proline Biosynthesis in Rice
    Sailila E. Abdula, Hye-Jung Lee, Hojin Ryu, Kwon Kyoo Kang, Illsup Nou, Mark E. Sorrells, Yong-Gu Cho
    Plant Molecular Biology Reporter.2016; 34(2): 501.     CrossRef
  • BrUGE1 transgenic rice showed improved growth performance with enhanced drought tolerance
    Sailila E. Abdula, Hye Jung Lee, Joonki Kim, Marjohn C. Niño, Yu-Jin Jung, Young-Chan Cho, Illsup Nou, Kwon-Kyoo Kang, Yong-Gu Cho
    Breeding Science.2016; 66(2): 226.     CrossRef
  • Molecular characterization of the UDP-glucose 4-epimerase (BrUGE) gene family in response to biotic and abiotic stress in Chinese cabbage (Brassica rapa)
    Yu Jin Jung, Jung Ho Kyoung, Ill Sup Nou, Yong Gu Cho, Kwon Kyoo Kang
    Plant Biotechnology Reports.2015; 9(6): 339.     CrossRef
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