Soybean is an economically important leguminous seed crop for feed and food products that are rich in seed protein, oil and saccharides. Also, black soybean has been known as medicinal food. However, Kunitz trypsin inhibitor (KTI) and lectin proteins are a main antinutritional factor in mature black soybean seed. The genetic removal of the KTI and lectin proteins will improve the nutritional value of black soybean seed. The
objective
of this research was to develop a new black soybean lines with double recessive allele for both KTI and lectin protein. A total of 179 F2 seeds were obtained from the cross of ‘Gaechuck#1’ (Le/Le, ti/ti genotype) and ‘LE-16’ (le/le, Ti/Ti genotype) parents. The segregation ratios for the presence or absence of KTI and lectin proteins in the F₂ seed generation were well fitted to an expected 3:1 ratio. The segregation ratios of 9:3:3:1 (113 Ti_Le_:28 Ti_lele:30 titiLe_:8 titilele) between Ti gene and Le gene in F₂ seed generation were observed (χ²=3.71, P=0.5–0.1). From eight F₂ seeds with double recessive allele (ti/ti, le/le genotype), one new strain (titilele, both KTI, and lectin protein free) was developed. New strain has black seed coat, black hilum and green cotyledon in mature seed and 100-seed weight was 19.5 g. This strain will be helpful in breeding program for selecting black soybean line with lacking both KTI and lectin protein.

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• Accumulation of triple recessive alleles for three antinutritional proteins in soybean with black seed coat and green cotyledon
  Gyung Young Kang, Sang Woo Choi, Won Gi Chae, Jong Il Chung
  Journal of Plant Biotechnology.2020; 47(2): 118.     CrossRef

dlm mutant newly identified displays necrotic spots with chlorotic halo despite the absence of any detectable pathogens in soybean. Kunitz trypsin inhibitor (KTI) protein of mature soybean seed is a main anti-nutritional factor and is controlled by Ti allele in soybean seed. The
objective
of this research was to determine the linkage or independent assortment between dlm allele and ti allele. F₂ population was made by crossing between “Gaechuck2ho” (DlmDlmtiti) and T363 (dlmdlmTiTi) parents. The normal leaf or disease lesion mimic leaf trait of parents and F₂ individual plants was recorded at R2 and R3 growth stage from field. The random seeds from individual F₂ plants harvested were analysed electrophoretically to determine the presence of the KTI protein. The disease lesion mimic trait was matched to 3 : 1 ratio at the 0.01 levels of probability. The absence of the KTI protein was controlled by a single recessive gene. In F₂ generation, the ratios of 102 Ti_Dlm_: 21 Ti_dlmdlm : 24 titiDlm_ : 4 titidlmdlm were observed. Segregation ratio gave a good fit to the 9 : 3 : 3 : 1 ratio based on F₂ generation at the 0.01 levels of probability. The data confirmed that dlm allele controlling disease lesion mimic trait was inherited independently with the ti allele controlling absence of KTI protein in soybean.

Citations

Citations to this article as recorded by  

• Accumulation of triple recessive alleles for three antinutritional proteins in soybean with black seed coat and green cotyledon
  Gyung Young Kang, Sang Woo Choi, Won Gi Chae, Jong Il Chung
  Journal of Plant Biotechnology.2020; 47(2): 118.     CrossRef
• Characterization and Rapid Gene-Mapping of Leaf Lesion Mimic Phenotype of spl-1 Mutant in Soybean (Glycine max (L.) Merr.)
  G Al Amin, Keke Kong, Ripa Sharmin, Jiejie Kong, Javaid Bhat, Tuanjie Zhao
  International Journal of Molecular Sciences.2019; 20(9): 2193.     CrossRef