Finger millet (Eleusine coracana L. Gaertn.) is an important cereal crop in eastern Africa and southern India with excellent grain storage capacity and the unique ability to thrive in extreme environmental conditions. In this study, we analyzed the genetic diversity and population structure of finger millet using 12 developed microsatellites. By sequencing 815 clones from an SSR-enriched genomic DNA library, we obtained 12 polymorphic SSR markers, which also revealed successful amplicons in finger millet accessions. Using the developed SSR markers, we estimated genetic diversity and population structure among 76 finger millet accessions in Asia, Africa, and unknown origins. The number of alleles ranged from 2 to 9, with an average of 3.3 alleles. The mean values of observed heterozygosity and expected heterozygosity were 0.27 and 0.35, respectively. The average polymorphism information content was 0.301 in all 76 finger millet accessions. AMOVA analysis showed that the percentage of molecular variance among the populations was 1%, that among individuals was 5%, and that within individuals was 94%. In STRUCTURE analysis, the 76 finger millet accessions were divided into two subpopulations which had an admixture of alleles. There was a correspondence among PCoA, AMOVA, and population structure. This study may form the basis for a finger millet breeding and improvement program.

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• Integrating path analysis and molecular markers to understand genetic diversity in finger millet, Eleusine coracana (L.) Gaertn.
  Mihir A. Hansalia, Vikas Pali, Amar A. Sakure, Sushil Kumar, Dinesh J. Parmar, Mihir M. Pandya, Dinesh Joshi
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• Development of iron and zinc transporter based genic SSR markers in foxtail millet and their cross- genera transferability in little millet
  Kumari Anjani, Kaushal Kumar, V. K. Sharma
  Cereal Research Communications.2026; 54(2): 875.     CrossRef
• Unlocking Climate Resilience Through Omics in Underutilized Small Millets
  S Muruganantham, Kannabiran Sakthivel, Chockalingam Vanniarajan, Paramasiwam Jeyaprakash, Subramaniam Geethanjali, Mathivanan Sivaji, Thanakkan Ramesh, Sadayandi Geethanjali, Selvavinayagam Monika, Lakshmanan Vigneshwari
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• Finger millet (Eleusine coracana) improvement: Challenges and prospects—A review
  Adane Gebreyohannes, Hussein Shimelis, Jacob Mashilo, Damaris A. Odeny, Taye Tadesse, Chris O. Ojiewo
  Plant Breeding.2024; 143(3): 350.     CrossRef
• Cross transferability of finger millet SSR markers to little millet (Panicum sumatrense Roth. Ex Roem & Schult.)
  Komal G. Lakhani, Kirankumar Suthar, Diwakar Singh, Sumankumar Jha, Harshal Patil, Rehana Niyaria
  Ecological Genetics and Genomics.2024; 32: 100281.     CrossRef
• Profiling of foxtail millet (Setaria italica L.) germplasm using in vitro drought screening and SSR markers
  N. S. Ahmad, M. A. Abid, A. A. Al-Assie
  Plant Genetic Resources: Characterization and Utilization.2022; 20(4): 277.     CrossRef
• Genome-Wide Assessment of Population Structure and Genetic Diversity of the Global Finger Millet Germplasm Panel Conserved at the ICRISAT Genebank
  C. Backiyalakshmi, Mani Vetriventhan, Santosh Deshpande, C. Babu, V. Allan, D. Naresh, Rajeev Gupta, Vania C. R. Azevedo
  Frontiers in Plant Science.2021;[Epub]     CrossRef

The present study aimed to determine the contents of five flavonols and two glycoalkaloids as well as the antioxidant activities of leaf ethanol extracts of 50 tomato accessions. The antioxidant activity was assessed using different tests: 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), 2,2-diphenyl-1-picryl hydrazyl (DPPH), nitric oxide (NO), and total polyphenol content (TPC). Flavonols and glycoalkaloids contents were determined using a high performance liquid chromatography-diode array detector system. Among five flavonols and two glycoalkaloids, naringenin and tomatine were detected in tomato leaves at high concentrations. Of the 50 tomato accessions, IT 229711, IT2365203, and IT 207224 were found to have the highest contents of quercetin, kaempferol, and tomatine, respectively. Leaf extract of IT189949 exhibited the highest relative antioxidant capacity index (RACI). Among the five flavonols, myricetin showed positive correlations with DPPH, ABTS, and NO, while isorhamnetin had positive correlation with DPPH. These results will expand the chemical constitution database and provide information on tomato leaves. They are valuable for the development of functional foods or feed-additives.

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• Protective role of α-tomatine against oxidative stress induced reactive oxygen species: In vitro radical scavenging assays
  
  International Journal of Biosciences (IJB).2025; : 123.     CrossRef
• Genetic Response of Solanum lycopersicum L. (Tomato) to Phytophthora infestans and Aspergillus niger
  Tavershima Moses Anakaa, Odunayo Joseph Olawuyi, Segun Gbolagade Jonathan
  International Journal of Plant Biology.2025; 16(1): 35.     CrossRef
• Extracts of tomatoes and potatoes as biopesticides: a review
  Joshua Ibukun Adebomi, Jianfeng Guo, Catherine Hui Niu
  Discover Agriculture.2025;[Epub]     CrossRef
• Solanaceae Leaves as are Sources of Antioxidants and Vitamin D
  Ekaterina Borodina, Oksana Kozlova, Veronika Boger, Larisa Proskuryakova, Vladimir Yustratov
  Food Processing: Techniques and Technology.2025; 55(1): 197.     CrossRef
• Bioformulation development via valorizing silica-rich spent mushroom substrate with Trichoderma asperellum for plant nutrient and disease management
  Garima Singh, Abhay Tiwari, Akansha Gupta, Ashwani Kumar, P. Hariprasad, Satyawati Sharma
  Journal of Environmental Management.2021; 297: 113278.     CrossRef
• Acute and Subacute Toxicity Profiles of the Methanol Extract of Lycopersicon esculentum L. Leaves (Tomato), a Botanical with Promising In Vitro Anticancer Potential
  Gaëlle S. Nguenang, Arsène S. M. Ntyam, Victor Kuete, Armando Zarrelli
  Evidence-Based Complementary and Alternative Medicine.2020;[Epub]     CrossRef

Sixty-nine Rubus accessions were analyzed to determine the genetic relationships using simple sequence repeat (SSR) markers. Twenty-three SSR markers generated a total of 351 alleles from all accessions, with an average of 15.3 alleles per locus. The average value of polymorphism information content was 0.76, ranging from 0.52 to 0.91. As a result of population structure analysis, 69 Rubus accessions of six Rubus species were subdivided into six subpopulations. Four subpopulations included distinct Rubus species accessions; pop2 (Rubus crataegifolius var. subcuneatus, 2 accessions), pop3 (R. crataegifolius Bunge., 18 accessions), pop4 (R. fruticosus L., 3 accessions) and pop6 (R. coreanus Miq., 36 accessions) while The pop1 and pop5 mainly included R. idaeus L. and R. parvifolius L., respectively. In cluster analysis, 69 Rubus accessions were divided into three groups. Group I contained 35 Rubus accessions, which consisted of six Rubus species. Groups II and III had 30 and 4 Rubus accessions, respectively. They consisted of only R. coreanus. The uncertain diversity of species and artificial groups of the Rubus genus has created confusion with respect to the correct classification of the species at both commercial and scientific levels. The results of the present study will provide basic information for phylogeny, taxonomy and breeding programs of Rubus species.

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• Genetic diversity and population structure of some blackberry genotypes collected from different parts of Türkiye using inter simple sequence repeat (ISSR) markers
  Fatma Alan, Aydın Uzun, Hasan Pınar
  Genetic Resources and Crop Evolution.2025; 72(7): 9001.     CrossRef
• Cross-transferability of Rubus ellipticus EST–SSR markers for genetic diversity analysis of peach (Prunus persica)
  Samriti Sharma, Rajinder Kaur, Krishan Kumar, Heerendra Sagar
  Genetic Resources and Crop Evolution.2024; 71(4): 1615.     CrossRef
• Genetic diversity and population relationships in wild Korean black raspberry (Rubus coreanus Miq.) based on microsatellite markers: establishing a fruit tree breeding strategy
  Sung-Kyung Han, Hanna Shin, Jei-Wan Lee, Kyung-Nak Hong, Ji-Young Ahn
  Horticulture, Environment, and Biotechnology.2024; 65(2): 293.     CrossRef
• Genetic differentiation between Czech and Norwegian raspberry populations: new options for breeding
  Jiří Sedlák, Leona Leišová-Svobodová, Inger Martinussen, Vojtěch Holubec
  Euphytica.2022;[Epub]     CrossRef
• Genetic variability in Rubus ellipticus collections assessed by morphological traits and EST-SSR markers
  Samriti Sharma, Rajinder Kaur, Krishan Kumar, Dinesh Kumar, Amol Kumar U. Solanke
  Journal of Plant Biochemistry and Biotechnology.2021; 30(1): 37.     CrossRef
• Evaluación de marcadores microsatélites (SSRs) heterólogos en Rubus niveus para estudios de diversidad genética en las Islas Galápagos
  Pablo Alarcón Bolaños, María de Lourdes Torres, Gabriela Pozo, María Paula Erazo, Mayra Ortega, Estefanía Rojas, Noelia Barriga, Antonio Leon Reyes
  ACI Avances en Ciencias e Ingenierías.2021; 13(2): 20.     CrossRef
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  Hilde Nybom, Gunārs Lācis
  Plants.2021; 10(2): 415.     CrossRef
• MODERN WAYS OF RASPBERRY BREEDING
  L. V. FROLOVA, T. A. HASHENKO, O. A. HASHENKO
  Fruit-Growing.2021; 33: 211.     CrossRef
• Genetic diversity of the Andean blackberry (Rubus glaucusBenth.) in Ecuador assessed by AFLP markers
  Patricia Garrido, Eduardo Morillo, Wilson Vásquez-Castillo
  Plant Genetic Resources: Characterization and Utilization.2020; 18(4): 243.     CrossRef
• Molecular markers in the genetic diversity studies of representatives of the genus Rubus L. and prospects of their application in breeding
  A. M. Kamnev, O. Yu. Antonova, S. E. Dunaeva, T. A. Gavrilenko, I. G. Chukhina
  Vavilov Journal of Genetics and Breeding.2020; 24(1): 20.     CrossRef
• Genetic and genomic resources for Rubus breeding: a roadmap for the future
  Toshi M. Foster, Nahla V. Bassil, Michael Dossett, Margaret Leigh Worthington, Julie Graham
  Horticulture Research.2019;[Epub]     CrossRef

Tomato (Solanum lycopersicum L.) is one of the most economically important plants in the family Solanaceae. Understanding its genetic diversity of accessions is vital for additional collection of tomato germplasms. The
objective
of this study was to determine the genetic diversity and population structure of 355 tomato accessions from Asia using 18 simple-sequence repeats (SSRs). A total of 176 alleles were detected at an average of ten alleles per SSR locus. The average major allele frequency and polymorphic information content were 0.69 and 0.39, respectively. Model-based structure analysis revealed two subpopulations (88%), including admixtures (11%) in the 355 Asian tomato accessions, consistent with clustering results based on genetic distance. The overall F_ST value was 0.135, indicating a moderate differentiation between the inferred subpopulations. Analysis of molecular variance showed that the genetic variance among geographical groups was less than 6%, in contrast to 86% of genetic variance among individuals. The results from this study will provide important information for future germplasm conservation and improvement programs for tomato.

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• Next generation sequencing technologies to explore the diversity of germplasm resources: Achievements and trends in tomato
  Pasquale Tripodi
  Computational and Structural Biotechnology Journal.2022; 20: 6250.     CrossRef
• Genetic diversity, population structure and validation of SSR markers linked to Sw-5 and I-2 genes in tomato germplasm
  Saidaiah Pidigam, Vishnukiran Thuraga, Suchandranath Babu Munnam, Geetha Amarapalli, Gopal Kuraba, Someswara Rao Pandravada, Srinivas Nimmarajula, Hari Kishan Sudini
  Physiology and Molecular Biology of Plants.2021; 27(8): 1695.     CrossRef

In order to estimate genetic diversity of Korean rice landraces, rice characters and SSR markers of 76 rice germplasms were analyzed. Of 12 rice characters, amylose content (AC) showed as largest variance (4.9 to 28.9%), whereas grain length (GL) showed as the lowest variance (4.4 to 5.9 mm). In principal component analyses (PCA), the first principal component explained 60.3% of total variance, in which culm length (CL) and growth period (GP) showed as positive variance and AC showed as negative variance. The second principal component explained an additional 22.4% of the total variance, in which GP and AC showed highly positive variables and CL showed a negative variable. Forty nine SSR markers produced a total of 473 alleles with an average of 9.65 alleles. Polymorphism information content (PIC) was in the range of 0.11 to 0.93. Average observed heterozygosity ranged from 0.12 to 0.39, with an average value of 0.61. As a result of STRUCTURE analysis, 76 Korean rice landraces showed two subpopulations. In clustering analysis, rice characters and SSR markers were clustered into four groups and three groups, respectively. However, they were not significant different from each other. These results provided insight into the characteristics of Korean rice landraces, thus improving our knowledge on rice breeding.

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• Genome-Wide Association Study to identify Bakanae disease resistance-related QTLs carrying novel candidate genes in rice (Oryza sativa L.)
  Yuting Zeng, Fang-Yuan Cao, Ah-Rim Lee, Dongryung Lee, Backki Kim, Soon-Wook Kwon
  npj Science of Plants.2025;[Epub]     CrossRef
• Physio-biochemical and molecular characterization for drought tolerance in rice genotypes at early seedling stage
  Al-Ashkar IM, Zaazaa EI, EL Sabagh A, Barutçular C
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