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Research Article

Environmental Stability and Correlation of Soybean Seed Starch with Protein and Oil Contents

Sanjeev K. Dhungana1, Krishnanand P. Kulkarni1, Minsu Kim1, Bo-Keun Ha2, Sungtaeg Kang3, Jong Tae Song1, Dong-Hyun Shin1, Jeong-Dong Lee1,4,*
Plant Breeding and Biotechnology 2017;5(4):293-303.
Published online: December 1, 2017

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*Corresponding author: Jeong-Dong Lee, jdlee@knu.ac.kr, Tel: +82-53-950-5709, Fax: +82-53-958-6880
• Received: September 22, 2017   • Revised: November 6, 2017   • Accepted: November 8, 2017

Copyright © 2017 The Korean Society of Breeding Science

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Environmental Stability and Correlation of Soybean Seed Starch with Protein and Oil Contents
Plant Breed. Biotech.. 2017;5(4):293-303.   Published online December 1, 2017
DOI: https://doi.org/10.9787/PBB.2017.5.4.293
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Environmental Stability and Correlation of Soybean Seed Starch with Protein and Oil Contents
Plant Breed. Biotech.. 2017;5(4):293-303.   Published online December 1, 2017
DOI: https://doi.org/10.9787/PBB.2017.5.4.293
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Environmental Stability and Correlation of Soybean Seed Starch with Protein and Oil Contents
Environmental Stability and Correlation of Soybean Seed Starch with Protein and Oil Contents

Description of 17 soybean genotypes, mean starch, crude protein and crude fat contents and starch group of each grownin 11 environments to evaluate the stability of seed starch content.

Genotype Starch group Mean starch content (%) Mean crude fat content (%) Mean crude protein content (%)
IT25334 High starch line 1.34 23.4 40.8
IT141778 High starch line 1.26 19.3 39.4
IT177744 High starch line 1.02 21.5 42.0
IT183905 High starch line 1.48 18.9 42.1
IT189276 High starch line 1.39 22.1 41.5
IT25344 Medium starch line 0.66 22.3 43.7
IT115763 Medium starch line 0.75 22.1 43.2
IT143112 Medium starch line 0.80 23.4 42.6
IT177234 Medium starch line 0.85 21.9 43.2
IT189205 Medium starch line 0.99 22.8 43.0
IT228277 Low starch line 0.24 21.9 45.7
IT228577 Low starch line 0.25 24.9 42.7
IT228620 Low starch line 0.35 22.4 43.9
IT229949 Low starch line 0.41 22.2 44.4
IT230194 Low starch line 0.41 22.1 44.0
Pungsannamul Check 0.64 16.8 42.0
Williams 82 Check 0.50 20.9 40.1

Geolocation information and planting dates (environments) at three locations in 2015 and 2016.

Location Geographic coordinates Planting datez)
2015 2016
Date1 Date2 Date1 Date2
Gunwi 36°07′N, 128°38′E 30 May (E1) 20 June (E2) 29 May (E7) NAy)
Gwangju 35°10′N, 126°54′E 2 June (E3) 25 June (E4) 29 May (E8) 23 June (E9)
Cheonan 36°49′N, 127°10′E 2 June (E5) 30 June (E6) 2 June (E10) 29 June (E11)

z)Letter and number in parenthesis for each planting date in various years are designated as environments.

y)NA: not available due to poor plant stands.

Analysis of variance for seed starch, crude protein, and crude fat contents of 17 soybean genotypes grown at two planting dates at three locations over two years.

Source of variation Starch Crude protein Crude fat
dfz) F value Pr df F value Pr df F value Pr
Year 1 136.12 < .0001 1 696.53 < .0001 1 6018.34 < .0001
Location 2 107.52 0.0092 2 136.16 0.0073 2 0.62 0.6166
Genotype 16 64.02 < .0001 16 68.44 < .0001 16 97.59 < .0001
Date 1 18.33 < .0001 1 0.19 0.6606 1 0.15 0.6986
Replication 1 0.1 0.7806 1 0.77 0.4717 1 0.01 0.9225
Year × genotype 16 6.92 < .0001 16 16 < .0001 16 42.94 < .0001
Year × location 2 14.18 < .0001 2 2.15 0.1192 2 58.95 < .0001
Location × genotype 32 3.98 < .0001 32 2.89 < .0001 32 2.53 < .0001
Genotype × date 16 3.03 0.0001 16 1.44 0.1274 16 1.26 0.2256
Year × location × genotype 32 2.17 0.0006 29 3.15 < .0001 29 2.18 0.001
Total 332 307 307

z)Degree of freedom.

Stability parameters range, thecoefficientof variation (CV), stability coefficient (bE), and stability coefficient of determination (r2) for mean seed starch content of 17 soybean genotypes calculated from 11 environments to evaluate the stability of seed starch among high, medium, and low starch genotypes.

Starch Group Genotype Mean (%) Range (%) CV (%) Stability coefficients Mean ranky)
bEz) P r2
High IT25334 1.34 1.94 48.15 2.32 < .0001 0.89 15
IT141778 1.26 2.06 41.84 1.55 0.036 0.49 13
IT177744 1.02 1.87 53.01 1.94 < .0001 0.85 16
IT183905 1.48 2.27 48.80 2.44 0.001 0.71 17
IT189276 1.39 2.11 36.58 1.74 < .0001 0.89 12
Mean 1.30 2.05 45.68 2.00 0.77
Medium IT25344 0.66 1.04 42.83 1.00 0.0002 0.80 10
IT115763 0.75 1.26 57.28 1.51 < .0001 0.94 14
IT143112 0.80 1.04 38.59 0.99 0.0002 0.84 9
IT177234 0.85 1.01 34.36 0.83 0.0049 0.60 8
IT189205 0.99 1.49 47.08 1.49 0.0028 0.69 11
Mean 0.81 1.17 44.03 1.16 0.77
Low IT228277 0.24 0.34 37.52 0.25 0.0173 0.48 3
IT228577 0.25 0.18 22.18 0.02 0.82 0.01 2
IT228620 0.35 0.19 14.81 0.00 0.9641 0.00 1
IT229949 0.41 0.58 30.30 0.35 0.0036 0.63 5
IT230194 0.41 0.39 35.00 0.32 0.0372 0.44 6
Mean 0.33 0.34 27.96 0.19 0.31
Check Pungsannamul 0.64 0.57 23.64 0.49 0.0001 0.88 4
Williams 82 0.50 0.82 34.27 0.31 0.1181 0.22 7
LSD0.05x) 0.18 0.24 11.46 0.41 0.29

z)bE was calculated from the regression of mean seed starch content of genotypes at each environments regressed on the environmental index.

y)Mean rank was calculated as the average value of three stability parameters range, CV, and bE.

x)LSD for comparison of means of each starch group.

Correlation among seed starch (SS), crude fat (CF), and crude protein (CP) contents of 17 soybean genotypes grown at two planting dates at three locations over two years (2015 and 2016).

Trait SS CF CP
SS 1
CF 0.1295* 1
CP −0.63**** −0.391**** 1

* and ****significant at the 0.05 and 0.0001 probability level, respectively.

Correlation of seed starch content with weather parameters during the seed-filling period (September and October).

Average daily mean temperature Average daily maximum temperature Average daily minimum temperature Average daily cloudiness
Correlation coefficient −0.92376 −0.26284 −0.91073 −0.71239
P value <.0001 0.4349 <.0001 0.0139
Table 1 Description of 17 soybean genotypes, mean starch, crude protein and crude fat contents and starch group of each grownin 11 environments to evaluate the stability of seed starch content.
Table 2 Geolocation information and planting dates (environments) at three locations in 2015 and 2016.

Letter and number in parenthesis for each planting date in various years are designated as environments.

NA: not available due to poor plant stands.

Table 3 Analysis of variance for seed starch, crude protein, and crude fat contents of 17 soybean genotypes grown at two planting dates at three locations over two years.

Degree of freedom.

Table 4 Stability parameters range, thecoefficientof variation (CV), stability coefficient (bE), and stability coefficient of determination (r2) for mean seed starch content of 17 soybean genotypes calculated from 11 environments to evaluate the stability of seed starch among high, medium, and low starch genotypes.

bE was calculated from the regression of mean seed starch content of genotypes at each environments regressed on the environmental index.

Mean rank was calculated as the average value of three stability parameters range, CV, and bE.

LSD for comparison of means of each starch group.

Table 5 Correlation among seed starch (SS), crude fat (CF), and crude protein (CP) contents of 17 soybean genotypes grown at two planting dates at three locations over two years (2015 and 2016).

significant at the 0.05 and 0.0001 probability level, respectively.

Table 6 Correlation of seed starch content with weather parameters during the seed-filling period (September and October).

Table 1

Table 2

Table 3

Table 4

Table 5

Table 6

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