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Research Article

SSR Analysis of Genetic Diversity and Cold Tolerance in Temperate Rice Germplasm

Jung-Pil Suh1, Young-Chan Cho1, Jeong-Heui Lee1, Sang-Bok Lee1, Jae-Youn Jung2, Im-Soo Choi1, Myeong-Ki Kim1, Chung-Kon Kim1, Kshirod K. Jena3,*
Plant Breeding and Biotechnology 2013;1(2):103-110.
Published online: June 30, 2013

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*Corresponding author: Kshirod K. Jena, k.jena@irri.org, Tel: +63-2-580-5600
• Received: May 21, 2013   • Revised: June 22, 2013   • Accepted: June 23, 2013

Copyright © 2013 The Korean Society of Breeding Science

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  • A total of 23 elite rice cultivars from eight countries were evaluated for cold tolerance using two screening methods at Chuncheon Substation, National Institute of Crop Science (NICS), Republic of Korea. The rice cultivars Jinbu, Mustaqillik, and Avangard showed cold tolerance and high spikelet fertility (63–79%) in cold-water irrigation screening. Under greenhouse screening, five cultivars (Giza 177, Avangard, Mustaqillik, Jinbu, and Jungan) showed high cold tolerance and high spikelet fertility (71–81%). Simple sequence repeat (SSR) marker analysis of 21 genotypes revealed two major clusters, the japonica and indica groups, with a genetic similarity of 0.69. Out of 21 rice cultivars, only four (Giza 178 from Egypt, Attey and Zakha from Bhutan, and Millin from Australia) fell under the indica cluster. The cold-tolerant varieties Jinbu, Mustaqillik, and Avangard were clustered with the japonica group, which had genetic similarity of 0.83. These varieties are considered as potential germplasm that will help diversify the japonica gene pool for cold-tolerant rice breeding. A one-way linear analysis of variance identified a significant relationship between individual alleles and traits. Three SSR markers were significantly associated with spikelet fertility under cold-water irrigation on chromosomes 1, 2, and 7. Five SSR markers were associated with spikelet fertility under a cool-environment greenhouse on chromosomes 8, 9, 10, and 12. The SSR markers associated with cold tolerance may also be useful as selection markers in indica/japonica cross combinations to improve cold tolerance.
  • Key words: Rice, Cold tolerance, SSR marker, Genetic diversity, Spikelet fertility
Rice cultivated in the temperate areas of East and Central Asia and in high altitudes is threatened by cold stress at the seedling, reproductive, and ripening stages (Mackill and Lei 1997). Cold stress causes significant yield loss in rice production (Murai et al. 1991; Nagasawa et al. 1994), thus enhancing cold tolerance through the use of a diverse germplasm of japonica rice is a very important objective in rice breeding. Japonica rice is more cold-tolerant than indica rice, and a wide variation in cold tolerance exists within the japonica rice germplasm (Jeong et al. 2000; Mackill and Lei 1997). Cold stress, however, is still a major constraint in rice production in temperate japonica rice cultivation areas because of the occurrence of high spikelet sterility induced by cold stress during meiosis (Lee 2001). To enhance cold tolerance in rice, it is therefore imperative to exchange and evaluate various rice germplasm from temperate rice-growing countries for cold stress at various growth stages in key japonica rice cultivation sites. Elite cold-tolerant rice germplasm also need to be crossed with a diverse set of elite cultivars from each country. However, there are limited genetic resources for the improvement of cold tolerance in temperate japonica rice cultivars. Some tropical japonica (javanica) cultivars have been reported to exhibit cold tolerance; and cold tolerance genes from the cultivars Silewah, Lambayque 1, and Padi Labou Alumbis have been introduced into many temperate japonica breeding lines (Abe et al. 1989; Glaszmann et al. 1990; Saito et al. 2001). It is then crucial to identify new genetic resources for cold tolerance to enable rice breeders to develop cold-tolerant rice cultivars for temperate regions. One of the projects of the Temperate Rice Research Consortium (TRRC) aims to develop improved breeding lines with cold tolerance and identify genetic characteristics related to cold tolerance through the exchange of elite germplasm among its member countries. It is very important to accurately evaluate and select cold-tolerant germplasm and use these as donor parents in rice breeding to improve cold tolerance. Various methods can be used to create cold injury environments to evaluate cold tolerance at the booting and reproductive stages in rice. However, screening several materials for cold tolerance is difficult, more so in producing reliable results. Therefore, it is necessary to use a reproducible phenotyping method to measure tolerant and sensitive traits under cold stress and apply improved QTL analysis methods to identify effective QTLs for cold tolerance at the reproductive stage. Screening rice genotypes by imposing cold-water irrigation on all growth stages in the field and by controlled air and water temperatures in the greenhouse, which allow correct measurement of traits associated with cold tolerance, is considered a reliable method of phenotyping for cold tolerance (Suh et al. 2010). The use of molecular markers has also made genetic similarity and dissimilarity of complex quantitative traits easier to understand. SSR markers produce a high frequency of polymorphic alleles in rice and are suitable for evaluating genetic diversity among closely related rice cultivars, including japonica rice (Suh et al. 2004). Extensive genetic studies and QTL analysis with DNA markers have been conducted on several mapping populations. QTL analyses have recently revealed that there are numerous QTLs for cold tolerance in the rice genome (Saito et al. 2001; Andaya and Mackill 2003; Xu et al. 2008; Suh et al. 2010; Ye et al. 2010). The objectives of this study are to identify promising cold-tolerant rice cultivars and to characterize the genetic relationship among the cold-tolerant japonica rice germplasm provided by the international partners of the TRRC.
Plant materials
A total of 23 rice cultivars submitted by member-countries of the working group for cold tolerance of the TRRC were used in this study for cold tolerance screening (Table 1). Six check cultivars composed of two indicas (IR64 and IR72), two Tongil-types (Milyang23 and Satbyeol), and two traditional Korean cultivars (Cheonwondo and Migwang) were also used in genetic diversity analysis.
Evaluation of cold tolerance under cold water irrigation
In the summer of 2009 and 2010, 23 rice cultivars from various countries were evaluated under cold stress through cold water (17°C) irrigation from maximum tillering to ripening stage at the Chuncheon Substation of NICS, RDA, Republic of Korea. The water temperature gradient was set at 17°C at the inlet to 24°C at the outlet. To screen for cold-tolerant genotypes, cold water was kept at a depth of 5 cm during the entire period of rice growth from tillering to grain maturity (June 20 to September 20). Eight agronomic traits were investigated: leaf discoloration (1–9), heading delay (days), culm length reduction (%), number of panicles, spikelets per panicle, seed fertility (%), panicle exsertion (1–9), and PA (phenotypic acceptability) at maturity (1–9). Phenotypic data on the percentage of spikelet fertility were collected from the first three panicles of five plants at the critical temperature zone in cold-water and normal-water plots. This screening procedure was performed following the method of Suh et al (2010).
Evaluation of cold tolerance under controlled-air-and-water-temperature conditions
Five seedlings each of the 23 rice cultivars were grown in plastic pots containing pulverized soil on 25 May. Extra tillers were removed from each plant, leaving just the main tiller to avoid overcrowding and to promote better growth. Three healthy plants showing uniform development stage were selected from each pot; one tiller from each of the three plants was tagged. The plants were then moved to the controlled-air-and-water-temperature greenhouse, maintained at 17°C, when the auricle of the flag leaf was approximately 4 cm inside the penultimate leaf of the tillers. After 10 days of cold treatment, the plants were taken back to the normal greenhouse and grown until maturity. The tillers bearing the tag were harvested at maturity and the average number of fertile grains (%) was measured. Screening using controlled cool-environment greenhouse conditions was performed following the method of Suh et al (2010).
SSR marker and statistical analysis
The 23 rice cultivars including six check varieties (composed of two indica, two Tongil-type (indica/japonica), and two traditional Korean japonica) were used for SSR analysis. Genomic DNA was extracted from the fresh frozen leaves of the rice cultivars by a modified CTAB method (Rogers and Bendich 1988). Eighty-six SSR markers associated with cold tolerance from previous studies were used in the polymorphism survey. The procedure used in the PCR assay was as described by Suh et al (2009). The PCR products were detected using a 4% denaturing polyacrylamide gel electrophoresis with silver staining. Generated DNA bands were analyzed and scored 1 for the presence- or 0 for the absence of allele. The similarity matrices obtained using the simple matching coefficient were subjected to unweighed pair-group methods using arithmetic average (UPGMA) clustering and represented in dendrogram form using NTSYS-pc program (Rohlf 1993). Linkage association analysis with cold tolerance was performed using ANOVA (one-way analysis of variance) and linear regression from SAS (SAS proc GLM).
Genetic diversity using SSR markers
Cluster analysis for the 23 rice cultivars and six check varieties was performed based on similarity coefficient matrices calculated from 86 SSR markers-derived data to generate a dendrogram (Fig. 1). These were grouped into two major clusters, japonica and indica rice, at 0.69 genetic similarity. Four rice varieties (Giza 178 from Egypt, Attey and Zakha from Bhutan, and Millin from Australia) were grouped into the indica cluster. Rice cultivars collected from high-altitude areas of Bhutan were very genetically different from the japonica and indica groups. Two cultivars collected from Bhutan, Paro Dumbja and Thimphu Dumbja, were clustered into the japonica group which had a genetic similarity of 0.76. Except for one variety from Egypt, the japonica rice cultivars collected from China, Egypt, and Korea formed one cluster. Two Korean traditional rice cultivars were included for cluster analysis to trace whether any of the tested cultivars were more similar with them than the others; which resulted to a small subgroup within the japonica group (Fig. 1).
Cold tolerance of collected rice cultivars under cold water irrigation
Various phenotypic performance responses of the 23 collected rice cultivars screened for cold tolerance were observed as indicated in Table 2. The leaf discoloration ranged from 3 to 8, (mean 5), and spikelet fertility in a cold water-irrigated plot (SFC) ranged from 10 to 79% (mean 38%). Korean japonica rice cultivars showed slightly higher estimates in cold tolerance traits against those of collected cultivars in both screening methods (Table 3). This was the reason Korean rice cultivars were selected to undergo these screening methods with selection criteria for cold tolerance that includes high spikelet fertility and leaf discoloration. Leaf discoloration was at 4–5 degrees in most varieties, 3 degrees in two (IR83222-F8-14 and Sobaeg), and 7–8 degrees in four (Attey, Paro Dumbja, Zakha, and Millin). No leaf discoloration was observed in most japonica rice cultivars, while severe discoloration was observed among the indica rice germplasm. Giza 178, clustered into the indica group, showed moderate cold tolerance and leaf discoloration; Paro Dumbja, clustered into the japonica group, showed a sensitive response. Attey, Zakha, Millin, and Giza 178 were included in the indica group for SSR analysis and showed typically susceptible phenotypes in leaf discoloration under cold water stress. Results show a significant difference in cold tolerance between indica and japonica rice at the seedling stage. It has been reported that indica rice tends to show less cold tolerance at the seedling stage (Mackill and Lei 1997). Although Paro Dumbja and Thimphu Dumbja belong to the japonica group, both showed severe leaf discoloration under cold water stress. Despite their affinity to japonica group, Paro Dumbja and Thimphu Dumbja were genetically very different from the other japonica rice cultivars. Indeed, genetic and phenotypic analysis revealed these two to be somewhat intermediate between temperate japonica and indica cultivars. Attey, Paro Dumbja, and Thimphu Dumbja were not investigated further for cold tolerance and agronomic traits because they did not flower under the environmental conditions of Korea (Tables 3 and 4).
Heading delays in cold-water treatment were less than 10 days in 13 varieties and 16–23 days in seven varieties. Three varieties, Attey, Paro Dumbja (white), and Thimphu Dumbja did not head in cold-water treatment. Culm length decreased by 20% in 11 varieties and by more than 30% in 4 varieties. Four varieties (Changbai 9, Giza 177, Kuban 3, and Amaroo) showed less than 20% of spikelet fertility from the cold-water irrigation screening. These cultivars showed a lower level of spikelet fertility due to cold stress than did indica cultivars tested in this study, which suggests that these cultivars are very susceptible to cold stress at a particular stage, such as the reproductive stage. Three varieties (Avangard, Mustaqillik, and Jinbu) showed more than 63% of spikelet fertility after cold-water treatment (inlet) in both 2009 and 2010. These varieties were also found to have 1–3 degrees of phenotypic acceptability at the ripening stage. Based on these results Avangard, Mustaqillik, and Jinbu are useful in the development of a cold-tolerant rice variety.
Cold tolerance of collected rice cultivars under controlled-air-and-water-temperature greenhouse
Five varieties (Giza 177 from Egypt, Avangard and Mustaqillik from Uzbekistan, and Jinbu and Jungan from Korea) had more than 71% spikelet fertility in a greenhouse with controlled air and water temperature. Giza 178, Zakha, and Millin were grouped as indica rice and proved to be highly susceptible to cold under these conditions. Three rice cultivars (Jinbu from Korea, and Avangard and Mustaqillik from Uzbekistan) showed stable cold tolerance from both screening methods. Avangard and Mustaqillik were of a taller plant type than other japonica rice cultivars, but most agronomic traits are adaptable to japonica rice breeding in the environmental condition of Korea (Table 4). These cultivars can be used as germplasm resource for improving cold tolerance during reproductive stage to sustain high spikelet fertility in japonica rice.
SSR markers associated with spikelet fertility under cold stress
The japonica sources of cold tolerance, IR66160-121-4-4-2, Jinbu, Silewah, Kunmingxizobaigu, and M202, were suitable for temperate japonica breeding or QTL mapping (Andaya and Mackill 2003; Kuroki et al. 2007; Takeuchi et al. 2001; Xu et al. 2008; Suh et al. 2010). Identification of new sources of cold tolerance, followed by the development of appropriate DNA markers associated with a cold tolerance phenotype for spikelet fertility, is an important breeding strategy in improving japonica cultivars at the temperate regions and high-altitude areas of the tropics (Suh et al. 2010). One-way and linear analysis of variance was conducted to identify significance in the relationship between individual alleles and traits (Table 5). Three SSR markers distributed on chromosomes 1, 2, and 7 were significantly associated with spikelet fertility under cold-water irrigation. Likewise, five SSRs distributed on chromosomes 8, 9, 10, and 12 were found associated with spikelet fertility under cool-air environment greenhouse. QTLs for cold tolerance at the booting and reproductive stages in rice were previously mapped on all of the chromosomes (Saito et al. 2001; Andaya and Mackill 2003; Xu et al. 2008; Suh et al. 2010; Ye et al. 2010). Spikelet fertility may be a critical factor associated with cold tolerance and yield under cold stress. Detection of these QTLs and their transfer into japonica rice cultivars may be valuable for breeders to substantially improve cold tolerance in japonica rice. The SSR markers associated with cold tolerance may be useful as selection markers in japonica cross combinations for improving cold tolerance. The promising cultivars Avangard, Mustaqillik, and Jinbu can be very useful as donors in developing mapping populations for identifying new QTL and improving the japonica breeding lines in cold tolerance research.
This study identified new sources of germplasm for improvement of cold tolerance in japonica rice. Of 23 cultivars used, three cultivars showed cold tolerance with high spikelet fertility in cold-water screening in the field, while five cultivars showed cold tolerance in cold-air-and-water treatment. Genetic diversity analysis using SSR markers grouped the germplasm into japonica and indica types. The number of SSR markers associated with spikelet fertility was three (located on chromosome 1, 2, and 7) under cold-water irrigation, and five (located on chromosome 8, 9, 10 and 12) under cold-air temperature stress. The three cold-tolerant cultivars identified in this study could be potential sources of germplasm to broaden the gene pool of japonica rice.
This work was supported with a grant from the National Institute of Crop Science (Project No. PJ008529), Rural Development Administration, Republic of Korea, and the Temperate Rice Research Consortium. We thank Ms. Leah Cruz, science editor, IRRI for editing the manuscript.
Fig. 1
Dendrogram of 29 collected rice cultivars for cold tolerance, clustered by similarity coefficients using 86 SSR markers.
pbb-01-103f1.jpg
Table 1
List of rice cultivars from eight countries used in this study.
Table 1
Rice cultivars Subspecies Origin
Jisheng 202 japonica China
Tongjing 29 japonica China
Changbai 9 japonica China
Giza 177 japonica Egypt
Giza 178 indica Egypt
Sakha 101 japonica Egypt
Avangard japonica Uzbekistan
Istiqbol japonica Uzbekistan
Mustaqillik japonica Uzbekistan
Severny japonica Russia
Kuban 3 japonica Russia
Liman japonica Russia
IR83222-F8-14 japonica IRRI
IR83222-F8-156 japonica IRRI
Jinbu japonica Korea
Jungan japonica Korea
Sobaeg japonica Korea
Attey indica Bhutan
Paro Dumbja japonica Bhutan
Thimphu Dumbja japonica Bhutan
Zakha indica Bhutan
Amaroo japonica Australia
Millin indica Australia
Total: 23 cultivars 8 countries
Table 2
Differences in morpho-agronomic traits of 23 rice cultivars under cold stress condition.
Table 2
Traits Mean ± SD Range
Leaf discoloration (1–9) 5 ± 1.51 3~8
Heading delay (days) 12 ± 6.16 5~23
Culm reduction (%) 22 ± 9.14 12~45
Number of panicle per plant 14 ± 2.53 10~18
Number of spikelet per panicle 114 ± 29.39 76~177
Panicle exsertion (1–9) 4 ± 1.56 1~7
PA-maturityz) (1–9) 6 ± 1.95 1~8
SFCy) (%) 38 ± 19.91 10~79
SFGx) (%) 59 ± 14.33 21~81

z)PA-maturity - phenotypic acceptability at maturing stage

y)SFC - spikelet fertility in cold-water irrigation plot

x)SFG - spikelet fertility in controlled cool-air environment greenhouse

Table 3
Genotypic differences in agronomic traits of collected rice germplasm under cold-water irrigation and controlled air/water conditions. All phenotypic data are mean values from 2009 and 2010 data.
Table 3
Rice cultivars Cold-water irrigationz)
LD (1–9) HD (days) CR (%) PN NSP PE (1–9) PAM (1–9) SFC (%) SFGy) (%) Mx) (%)
Jisheng 202 6 8 18 11 152 4 7 34 53 44
Tongjing 29 4 7 20 17 121 1 8 27 61 44
Changbai 9 5 9 15 13 130 3 7 17 52 35
Giza 177 4 20 27 14 77 6 7 12 76 44
Giza 178 5 23 45 16 96 7 7 40 21 31
Sakha 101 3 17 22 17 76 7 9 10 54 32
Avangard 5 8 14 11 106 4 3 63 77 70
Istiqbol 5 7 16 10 144 3 5 58 57 58
Mustaqillik 5 5 15 11 82 4 3 69 72 70
Severny 6 6 12 10 163 3 5 45 52 49
Kuban 3 6 7 23 15 127 4 8 12 67 40
Liman 6 6 12 12 177 4 7 36 55 46
IR83222-F8-14 4 22 15 14 105 3 5 32 63 47
IR83222-F8-156 5 21 19 13 101 3 4 34 68 51
Jinbu 4 11 13 14 123 3 1 79 81 80
Jungan 4 12 18 17 92 4 6 46 71 58
Sobaeg 4 12 24 14 134 4 6 40 51 46
Attey 8 - - - - - - - - -
Paro Dumbja 8 - - - - - - - - -
Thimphu Dumbja 7 - - - - - - - - -
Zakha 8 20 33 17 84 5 5 59 45 52
Amaroo 5 11 36 14 85 6 8 18 56 37
Millin 8 16 34 18 108 7 8 25 38 31

z)LD - leaf discoloration, HD - heading delay, CR - culm reduction, PN - number of panicle per plant, NSP - number of spikelet per panicle, PE - panicle exsertion, PAM - phenotypic acceptability at maturing stage, SFC - spikelet fertility in cold-water irrigation plot

y)SFG - spikelet fertility in controlled cool-air environment greenhouse

x)M - mean value of SFC and SFG

Table 4
Agronomic traits of collected cold-tolerant rice accessions under natural conditions at Chuncheon, Republic of Korea.
Table 4
Rice accessions DTHz) (M.D) CL (cm) PL (cm) PN NSP Fer (%)
Ji Sheng 202 7.25 78 21 14 145 96
Tong Jing 29 7.24 80 22 13 123 97
Chang Bai 9 7.23 77 19 11 139 95
Giza 177 8.8 74 19 13 84 95
Giza 178 8.17 66 24 15 137 94
Sakha 101 9.6 54 20 13 129 87
Avangard 8.5 102 23 10 87 97
Istiqbol 8.7 114 23 7 140 88
Mustaqillik 8.11 99 23 11 71 95
Severny 7.24 87 17 10 182 95
Kuban 3 7.18 90 21 10 130 96
Liman 7.25 83 17 11 164 83
SR30084-F8-14 8.25 86 23 10 107 96
SR30084-F8-156 8.9 86 21 10 88 90
Jinbu 7.30 79 19 15 108 98
Jungan 8.13 87 20 14 84 96
Sobaeg 7.29 75 21 12 107 96
Attey - - - - - -
Paro Dumbja - - - - - -
Thimphu Dumbja - - - - - -
Zakha 8.30 114 28 13 75 97
Amaroo 8.25 81 20 11 97 94
Millin 8.7 74 24 13 99 81

z)DTH - days to heading, CL - culm length, PL - panicle length, PN - number of panicle per plant, NSP - number of spikelet per panicle, Fer - spikelet fertility

Table 5
SSR markers associated with spikelet fertility for cold tolerance.
Table 5
Traits Linked SSR marker Chr. F-value No. of alleles
SFCz RM3475 1 7.01** 7
(%) RM5764 2 9.47** 2
RM481 7 7.63** 10
SFGy RM256 8 18.56** 2
(%) RM447 8 10.62** 3
RM1026 9 10.14** 4
RM590 10 10.16** 3
RM512 12 10.34** 3

z)SFC - spikelet fertility in cold-water irrigation plot

y)SFG - spikelet fertility in controlled cool-environment greenhouse

**- significant at 0.01 probabilities

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SSR Analysis of Genetic Diversity and Cold Tolerance in Temperate Rice Germplasm
Plant Breed. Biotech.. 2013;1(2):103-110.   Published online June 30, 2013
DOI: https://doi.org/10.9787/PBB.2013.1.2.103
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SSR Analysis of Genetic Diversity and Cold Tolerance in Temperate Rice Germplasm
Plant Breed. Biotech.. 2013;1(2):103-110.   Published online June 30, 2013
DOI: https://doi.org/10.9787/PBB.2013.1.2.103
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SSR Analysis of Genetic Diversity and Cold Tolerance in Temperate Rice Germplasm
Image
Fig. 1 Dendrogram of 29 collected rice cultivars for cold tolerance, clustered by similarity coefficients using 86 SSR markers.

Fig. 1

SSR Analysis of Genetic Diversity and Cold Tolerance in Temperate Rice Germplasm

List of rice cultivars from eight countries used in this study.

Rice cultivars Subspecies Origin
Jisheng 202 japonica China
Tongjing 29 japonica China
Changbai 9 japonica China
Giza 177 japonica Egypt
Giza 178 indica Egypt
Sakha 101 japonica Egypt
Avangard japonica Uzbekistan
Istiqbol japonica Uzbekistan
Mustaqillik japonica Uzbekistan
Severny japonica Russia
Kuban 3 japonica Russia
Liman japonica Russia
IR83222-F8-14 japonica IRRI
IR83222-F8-156 japonica IRRI
Jinbu japonica Korea
Jungan japonica Korea
Sobaeg japonica Korea
Attey indica Bhutan
Paro Dumbja japonica Bhutan
Thimphu Dumbja japonica Bhutan
Zakha indica Bhutan
Amaroo japonica Australia
Millin indica Australia
Total: 23 cultivars 8 countries

Differences in morpho-agronomic traits of 23 rice cultivars under cold stress condition.

Traits Mean ± SD Range
Leaf discoloration (1–9) 5 ± 1.51 3~8
Heading delay (days) 12 ± 6.16 5~23
Culm reduction (%) 22 ± 9.14 12~45
Number of panicle per plant 14 ± 2.53 10~18
Number of spikelet per panicle 114 ± 29.39 76~177
Panicle exsertion (1–9) 4 ± 1.56 1~7
PA-maturityz) (1–9) 6 ± 1.95 1~8
SFCy) (%) 38 ± 19.91 10~79
SFGx) (%) 59 ± 14.33 21~81

z)PA-maturity - phenotypic acceptability at maturing stage

y)SFC - spikelet fertility in cold-water irrigation plot

x)SFG - spikelet fertility in controlled cool-air environment greenhouse

Genotypic differences in agronomic traits of collected rice germplasm under cold-water irrigation and controlled air/water conditions. All phenotypic data are mean values from 2009 and 2010 data.

Rice cultivars Cold-water irrigationz)
LD (1–9) HD (days) CR (%) PN NSP PE (1–9) PAM (1–9) SFC (%) SFGy) (%) Mx) (%)
Jisheng 202 6 8 18 11 152 4 7 34 53 44
Tongjing 29 4 7 20 17 121 1 8 27 61 44
Changbai 9 5 9 15 13 130 3 7 17 52 35
Giza 177 4 20 27 14 77 6 7 12 76 44
Giza 178 5 23 45 16 96 7 7 40 21 31
Sakha 101 3 17 22 17 76 7 9 10 54 32
Avangard 5 8 14 11 106 4 3 63 77 70
Istiqbol 5 7 16 10 144 3 5 58 57 58
Mustaqillik 5 5 15 11 82 4 3 69 72 70
Severny 6 6 12 10 163 3 5 45 52 49
Kuban 3 6 7 23 15 127 4 8 12 67 40
Liman 6 6 12 12 177 4 7 36 55 46
IR83222-F8-14 4 22 15 14 105 3 5 32 63 47
IR83222-F8-156 5 21 19 13 101 3 4 34 68 51
Jinbu 4 11 13 14 123 3 1 79 81 80
Jungan 4 12 18 17 92 4 6 46 71 58
Sobaeg 4 12 24 14 134 4 6 40 51 46
Attey 8 - - - - - - - - -
Paro Dumbja 8 - - - - - - - - -
Thimphu Dumbja 7 - - - - - - - - -
Zakha 8 20 33 17 84 5 5 59 45 52
Amaroo 5 11 36 14 85 6 8 18 56 37
Millin 8 16 34 18 108 7 8 25 38 31

z)LD - leaf discoloration, HD - heading delay, CR - culm reduction, PN - number of panicle per plant, NSP - number of spikelet per panicle, PE - panicle exsertion, PAM - phenotypic acceptability at maturing stage, SFC - spikelet fertility in cold-water irrigation plot

y)SFG - spikelet fertility in controlled cool-air environment greenhouse

x)M - mean value of SFC and SFG

Agronomic traits of collected cold-tolerant rice accessions under natural conditions at Chuncheon, Republic of Korea.

Rice accessions DTHz) (M.D) CL (cm) PL (cm) PN NSP Fer (%)
Ji Sheng 202 7.25 78 21 14 145 96
Tong Jing 29 7.24 80 22 13 123 97
Chang Bai 9 7.23 77 19 11 139 95
Giza 177 8.8 74 19 13 84 95
Giza 178 8.17 66 24 15 137 94
Sakha 101 9.6 54 20 13 129 87
Avangard 8.5 102 23 10 87 97
Istiqbol 8.7 114 23 7 140 88
Mustaqillik 8.11 99 23 11 71 95
Severny 7.24 87 17 10 182 95
Kuban 3 7.18 90 21 10 130 96
Liman 7.25 83 17 11 164 83
SR30084-F8-14 8.25 86 23 10 107 96
SR30084-F8-156 8.9 86 21 10 88 90
Jinbu 7.30 79 19 15 108 98
Jungan 8.13 87 20 14 84 96
Sobaeg 7.29 75 21 12 107 96
Attey - - - - - -
Paro Dumbja - - - - - -
Thimphu Dumbja - - - - - -
Zakha 8.30 114 28 13 75 97
Amaroo 8.25 81 20 11 97 94
Millin 8.7 74 24 13 99 81

z)DTH - days to heading, CL - culm length, PL - panicle length, PN - number of panicle per plant, NSP - number of spikelet per panicle, Fer - spikelet fertility

SSR markers associated with spikelet fertility for cold tolerance.

Traits Linked SSR marker Chr. F-value No. of alleles
SFCz RM3475 1 7.01** 7
(%) RM5764 2 9.47** 2
RM481 7 7.63** 10
SFGy RM256 8 18.56** 2
(%) RM447 8 10.62** 3
RM1026 9 10.14** 4
RM590 10 10.16** 3
RM512 12 10.34** 3

z)SFC - spikelet fertility in cold-water irrigation plot

y)SFG - spikelet fertility in controlled cool-environment greenhouse

**- significant at 0.01 probabilities

Table 1 List of rice cultivars from eight countries used in this study.
Table 2 Differences in morpho-agronomic traits of 23 rice cultivars under cold stress condition.

PA-maturity - phenotypic acceptability at maturing stage

SFC - spikelet fertility in cold-water irrigation plot

SFG - spikelet fertility in controlled cool-air environment greenhouse

Table 3 Genotypic differences in agronomic traits of collected rice germplasm under cold-water irrigation and controlled air/water conditions. All phenotypic data are mean values from 2009 and 2010 data.

LD - leaf discoloration, HD - heading delay, CR - culm reduction, PN - number of panicle per plant, NSP - number of spikelet per panicle, PE - panicle exsertion, PAM - phenotypic acceptability at maturing stage, SFC - spikelet fertility in cold-water irrigation plot

SFG - spikelet fertility in controlled cool-air environment greenhouse

M - mean value of SFC and SFG

Table 4 Agronomic traits of collected cold-tolerant rice accessions under natural conditions at Chuncheon, Republic of Korea.

DTH - days to heading, CL - culm length, PL - panicle length, PN - number of panicle per plant, NSP - number of spikelet per panicle, Fer - spikelet fertility

Table 5 SSR markers associated with spikelet fertility for cold tolerance.

SFC - spikelet fertility in cold-water irrigation plot

SFG - spikelet fertility in controlled cool-environment greenhouse

- significant at 0.01 probabilities

Table 1

Table 2

Table 3

Table 4

Table 5

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