Powdery mildew (PM), caused by the biotrophic fungus Podosphaera xanthii, drastically reduces the yield and quality of melon (Cucumis melo L.). Knowledge of the genetic control and high throughput molecular markers linked with resistance against this disease are essential for breeding programs. The bioassay study of the F1 and F2 populations derived from the parents, ‘PMR 5’ (♂) and ‘SCNU1154’ (♀) revealed a monogenic dominant nature of resistance to the devastating race, race 5. Besides, we developed three SNP based high resolution melting markers, PMm-HRM-1, PMm-HRM-2, and PMm-HRM-3, based on the previously identified SNPs on chromosome 12 and validated them using 8 melon lines and 137 F2 populations. Among these, the SNP of marker PMm-HRM-1 causes a missense mutation in the LRR region of MELO3C002393 and we were able to distinguish the resistant vs susceptible genotypes from eight diverse melon accessions and the segregating F2 population with more than 90% genotyping efficiency. The other two markers were based on intergenic SNPs and had more than 80% genotyping efficiency in F2 population. These markers will be helpful to melon breeders to develop melon cultivars resistant to P. xanthii race 5 via marker assisted breeding programs.

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• Developing DNA Markers based on Male-Specific Chromosomal Regions for Selecting Male Plants in Hop (Humulus lupulus)
  Tae hyun Ha, Jae Il Lyu, So Young Yi, Si-Yong Kang
  Plant Breeding and Biotechnology.2024;[Epub]     CrossRef
• Identification of Gene Responsible for Conferring Resistance against Race KN2 of Podosphaera xanthii in Melon
  Sopheak Kheng, San-Ha Choe, Nihar Sahu, Jong-In Park, Hoy-Taek Kim
  International Journal of Molecular Sciences.2024; 25(2): 1134.     CrossRef

Cherry tomatoes are highly nutritious, flavory with a pleasant taste and are becoming increasingly popular to the consumers. The cherry tomato cv. ‘Minichal’ produced some dwarf plants along with normal plants. Lignin, a phenolic biopolymer is the key component of cell walls in plants. Here, we analyzed lignin biosynthesis-related genes in leaves, inflorescences and fruits of dwarf and normal cherry tomato plants by reverse-transcription quantitative PCR (RT-qPCR). Among analyzed genes, SlCCOAOMT1, SlCCOAOMT2, SlCCOAOMT3, SlF5H, and SlCOMT showed significantly higher expressions, in leaf and inflorescence of dwarf plants compared with the normal plants, while SlPAL1 showed a significantly higher expression only in the leaves. On the contrary, SlHCT and SlC3H showed significantly lower expression levels in the leaves and inflorescences of dwarf plants compared with normal ones. The results suggest that SlHCT and SlC3H might have an impact on the dwarf cherry tomato plants.

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• Joint Impacts of Meloidogyne incognita and Soil Nutrition on Solanum lycopersicum var. cerasiforme
  Lei Wang, Xingfu Yan, Zhanhui Tang
  Plant Disease.2024; 108(5): 1252.     CrossRef
• A response of biomass and nutrient allocation to the combined effects of soil nutrient, arbuscular mycorrhizal, and root-knot nematode in cherry tomato
  Lei Wang, Xin Chen, Xingfu Yan, Congli Wang, Pingting Guan, Zhanhui Tang
  Frontiers in Ecology and Evolution.2023;[Epub]     CrossRef
• Nutrients Regulate the Effects of Arbuscular Mycorrhizal Fungi on the Growth and Reproduction of Cherry Tomato
  Lei Wang, Xin Chen, Yeqin Du, Di Zhang, Zhanhui Tang
  Frontiers in Microbiology.2022;[Epub]     CrossRef

Molecular genetic studies of self-incompatibility (SI) are the most accentuating part in the way of advancement of reproductive mechanisms in flowering plants. In the Brassicaceae plants, self-incompatibility has been mapped genetically to a single chromosomal location where several closely linked genes have been identified. Recently, various studies have provided a novel insight into the basis of specificity in the S-receptor kinase (SRK) and S-locus protein 11 or S-locus Cysteine-rich (SP11/SCR) interaction, the nature of the signaling cascade that culminates in the inhibition of ‘self’ pollen, and the physiological and morphological changes that are associated with transitions between the outbreeding and inbreeding modes of mating in the Brassicaceae. In this review, we discuss the current view of the molecular genetic aspects of the self-incompatibility in Brassicaceae.

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• Genetics Behind Sexual Incompatibility in Plants: How Much We Know and What More to Uncover?
  Sukanya Chakraborty, Smritikana Dutta, Malay Das
  Journal of Plant Growth Regulation.2023; 42(11): 7164.     CrossRef
• Assessment of genotypic variation and self-incompatibility in cauliflower (Brassica oleracea var. botrytis) genotypes
  
  International Journal of Biosciences (IJB).2020; : 173.     CrossRef
• Progress on deciphering the molecular aspects of cell-to-cell communication in Brassica self-incompatibility response
  Nidhi Sehgal, Saurabh Singh
  3 Biotech.2018;[Epub]     CrossRef

Cabbage (Brassica oleracea) is a very important vegetable worldwide and biotic stress is a crucial issue for this crop. Enhancement of resistance by exploiting stress resistance-related genes offers the most efficient approach to address this issue. Among the stress resistance-related genes, thaumatin-like proteins (TLPs) play a vital role in enhancement of resistance against stresses. In this study, we identified 12 TLPs from B. oleracea genomic DNA sequencing database, analyzed their sequences and compared with other published pathogenesis-related TLPs, and found a high degree of homology with them. In addition, these genes showed an organ-specific expression, three of which expressed differentially after Pectobacterium carotovorum subsp. carotovorum infection in cabbage plants. Data obtained in this study suggest the probable involvement of TLPs in resistance against soft rot disease of Brassica.

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• Antifungal activity of citral against Fusarium wilt in tomatoes and induction of the upregulation of glucanase, chitinase, and thaumatin-like protein plant defense genes
  Portia Osei-Obeng, Leonard Muriithi Kiirika, Aggrey Bernard Nyende
  Journal of Natural Pesticide Research.2024; 10: 100097.     CrossRef
• Genome-wide comprehensive characterization and expression analysis of TLP gene family revealed its responses to hormonal and abiotic stresses in watermelon (Citrullus lanatus)
  Chet Ram, Shagufta Danish, Mahipal Singh Kesawat, Bhupendra Singh Panwar, Manjusha Verma, Lalit Arya, Sheel Yadav, Vedprakash Sharma
  Gene.2022; 844: 146818.     CrossRef
• Molecular Characterisation of Soybean Osmotins and Their Involvement in Drought Stress Response
  Giulia Ramos Faillace, Paula Bacaicoa Caruso, Luis Fernando Saraiva Macedo Timmers, Débora Favero, Frank Lino Guzman, Ciliana Rechenmacher, Luisa Abruzzi de Oliveira-Busatto, Osmar Norberto de Souza, Christian Bredemeier, Maria Helena Bodanese-Zanettini
  Frontiers in Genetics.2021;[Epub]     CrossRef
• Genome-wide analysis and evolution of plant thaumatin-like proteins: a focus on the origin and diversification of osmotins
  Giulia Ramos Faillace, Andreia Carina Turchetto-Zolet, Frank Lino Guzman, Luisa Abruzzi de Oliveira-Busatto, Maria Helena Bodanese-Zanettini
  Molecular Genetics and Genomics.2019; 294(5): 1137.     CrossRef
• Alfin-like transcription factor family: characterization and expression profiling against stresses in Brassica oleracea
  Md. Abdul Kayum, Jong-In Park, Nasar Uddin Ahmed, Gopal Saha, Mi-Young Chung, Jong-Goo Kang, Ill-Sup Nou
  Acta Physiologiae Plantarum.2016;[Epub]     CrossRef
• cDNA-AFLP analysis reveals the adaptive responses of citrus to long-term boron-toxicity
  Peng Guo, Yi-Ping Qi, Lin-Tong Yang, Xin Ye, Huan-Xin Jiang, Jing-Hao Huang, Li-Song Chen
  BMC Plant Biology.2014;[Epub]     CrossRef