Black soybeans are valued for their rich nutritional content and potential health benefits, attributed to their functional components that enhance antioxidant activity. In this study, we evaluate and compare the isoflavone and anthocyanin content, as well as the antioxidant potential, of seven Korean black soybean genotypes. Isoflavone content ranged from 2,032.8 to 3,536.8 µg/g, with Soman displaying the highest levels of both aglycones and glucosides, indicating notable bioactive potential. In terms of anthocyanins, Danheuk had the highest total content (24,080.6 µg/g), while Soman excelled in Pelargonidin-3- glucoside (Pg3glc). Soman also showed superior antioxidant activity across all measures, including total polyphenol, flavonoid content, as well as radical scavenging abilities (ABTS and DPPH). Strong correlations were found between total flavonoid content, total polyphenol content, genistin, total isoflavone content and antioxidant activity, while correlations with total anthocyanins were relatively weaker. These findings reveal significant genetic variability in isoflavone and anthocyanin content among soybean genotypes, with Soman showing particularly high antioxidant potential, suggesting its value for health-related applications and soybean breeding programs.
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Foxglove aphid (FA),
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Soybean [
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The conventional soybean breeding program by single seed descent method required around 8 to 9 years to develop a cultivar. Through the advancement of breeding techniques, such as marker-assisted selection, required resources could be significantly saved, but the generation advancement step still slows down the entire soybean breeding program time course. In this study, 28 soybean varieties were tested to find the optimal speed breeding conditions for soybeans that could rapidly advance one generation with 2 light sources, 3 light conditions, and 2 planting densities. Plants were kept under short-day conditions (9 hours light/15 hours dark). We optimized the growth conditions for shortening the period of soybean generation progression based on speed breeding. The optimizing conditions are as follows. (1) Irradiation using LED light source for 9 hours, (2) 506 mmol/(m2∙s) of PPFD at 30 cm from the ground, (3) Planting density of 5 cm × 5 cm, (4) temperature of 25℃ ± 2℃ and (5) humidity of 50% ± 10%. If this condition is used, soybeans can be advanced by one generation within an average of 73 days. It is possible to advance five generations a year using only indoor speed-breeding system. Furthermore, if it includes the development of lines in the field, four generation per year, which is advance three generations using indoor speed-breeding system and one generation in the field, is allowed to increase soybean breeding speed with minimum input.
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The number of four-seeded pods is a plant trait that is of great interest in terms of increasing soybean production. The
objective
of this study was to understand the agronomic characteristics of four-seeded pods of FS1159, which contain a significantly higher ratio of four-seeded pods than do other genotypes. FS1159 showed a significantly lower ratio of one- and two-seeded pods and a significantly higher ratio of three- (39.6%) and four- (11.3%) seeded pods than did the four check soybeans. The average values of the traits of FS1159 in this study were: plant height, 58.1 cm; the number of nodes, 15.7; the number of branches, 6.5; and 100-seed weight, 20.3 g. These results indicate that FS1159 can be used as a new genetic resource to explore the traits of four-seeded-pod and improve the soybean yield.
From an agricultural point of view, deep learning models can be used in a variety of way to study the agricultural properties of soybean. Object detection can be performed using image or video data on phenotypic traits of soybean. In this project, a study on the phenotype analysis about soybean seed was conducted by artificial intelligence (AI) based on the YOLOv5 model. In model summary, layers and parameters were calculated as 243 and 7020913, respectively. Means of average precision (mAP)@[0.5: 0.95] was recorded as 0.835, 0.739, 0.785 for each class, and Daewonkong (DW) with yellow seed coat color was calculated as the highest value, and landrace with black seed coat color (NG2) revealed the lowest value. As a result of prediction performance in the confusion matrix, each class of DW, NG2, and inbreeding line with green seed coat color (NGT) showed significant correlation of true positive (TP) in the matrix with the same output value for the input value.
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Germplasm screening is time-consuming, labor-intensive, and expensive. Therefore, it is important to develop the most effective selection method for plant breeding and genetic studies. Herein, experiments were conducted to determine the optimal growth stage and application dose of bentazone based on the response of soybeans. The influence of soybean accessions, application doses (0X, 1X and 2X), and growth stages (VC, V1, V2) on plant injury and growth performance was examined. Leaf injury and growth performance were significantly influenced by accession, dose, and growth stage. There was a substantial difference of 2%-3% and 23%-31% in leaf necrosis between the tolerant and susceptible groups, respectively. The mean leaf necrosis and visual scores for the VC, V1, and V2 stages showed a similar pattern, with a non-significant difference. The effect of accessions on plant height, fresh weight, and dry weight reduction had no significant difference in all growth stages. No significant differences in necrosis were found in the tolerant accessions across bentazone doses, while significant differences were found in the susceptible accessions. The leaf necrosis from 2X bentazone was almost double that of 1X treatment. However, there was no difference in visual scores between the 1X and 2X doses. In addition, the visual scores showed no difference between the 1X and 2X doses within the growth stages. The application of 1X dose bentazone at the V1 stage is suitable to determine tolerant and susceptible soybeans. This result can help determine the genotypes for breeding and genetic studies.
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The soluble sugar content of legume seeds affects the final flavor of the legume and legume products. The purpose of this study was to develop a rapid, simple, and low-cost colorimetric method for high-throughput screening of sucrose content in legume seeds. This colorimetric method was based on the enzymatic reactions of invertase (INV) and glucose oxidase (GOD). Briefly, 20 different soybean and cowpea varieties were used in this study. For sucrose extraction, solvent-to-sample ratios of 10:1 and 5:1 were found to result in optimal absorbance values for determining sucrose content in soybean and cowpea, respectively. The extraction efficiency was also evaluated under various extraction temperatures (25℃ and 50℃) and incubation times (15 minutes, 2 hours, 8 hours, and 24 hours) and the sucrose content was found to increase with increasing temperature and time. Accordingly, the optimal extraction conditions were 24 hours of incubation at 50℃. Under this condition, the GOD/INV method had significant positive correlations (
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Several environmental and genetic factors affect the isoflavone and anthocyanin contents in soybeans. This study aimed to assess the influences of cotyledon color and harvest period on the contents of five major isoflavones and three major anthocyanins in 323 black soybean landraces grown in Korea. In all the soybeans, malonylgenistin, malonyldaidzin and cyanidin-3-
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Lipoxygenase-2 present in soybean seeds is the prime contributor to off-flavour generated during the processing of soy products. Genetic elimination of this undesirable component is important as the heat inactivation not only incurs extra cost but also affects the protein solubility. The present study was aimed at eliminating lipoxygenase-2 from cultivar ‘JS97-52’ through marker assisted introgression of null allele of
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Phytophthora root and stem rot (PRR) of soybean is a severe disease that causes significant economic losses in soybean-growing countries. The causal agent,
objective
s of this review are to summarize published studies conducted on PRR, to suggest future directions of genetic researches and breeding to the target pathogen. This review will discuss the followings; i) a brief introduction to PRR and the causal agent
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We have conducted this study to select soybeans adaptable to North Korea using growing degree days (GDD) as a heuristic tool. For analyzing climatic conditions, we assumed that climatic conditions of Longjing, Donggang, and Yeoncheon are similar to the Eastern Coast Northern Zone, Suyangsan Northern Zone, and Suyangsan Southern Zone in North Korea, respectively. The predicted days to flowering of 100 soybeans grown at Longjing, Donggang, and Yeoncheon were in ranges from 7/17 to 7/30, from 7/16 to 7/28, and from 7/30 to 8/10, respectively, while the last date of flowering for completely harvesting were predicted by July 12th, August 2nd, and August 11th, respectively. The predicted days to flowering using GDD were almost similar to the days investigated in the three fields. These results showed that GDD is highly effective in predicting development rates of soybean, such as days to flowering and days to maturity. Although most of the tested soybeans were difficult to adapt to Longjing, we selected 12, 15, and 16 soybean varieties/lines based on the predicted days to flowering and last date of flowering for completely harvesting and yield of the soybeans grown at Longjing, Donggang and Yeoncheon, respectively, for further analyzing effects of the regional characteristics on agricultural traits of the selected soybeans. The 100-seed weight and yield of the selected soybeans grown at Longjing was significantly low compared with those at the other locations. The results implied that the soybeans would be exposed to freezing temperature before R6 stage and the reduced size would contribute to soybean yield loss. Taken together, we concluded that Suwon212, Iksan13 and ‘Shinpaldalkong2’ which were stable in degree of lodging, 100-seed weight, and yield at Donggang and Yeoncheon could be adaptable to Suyangsan Northern Zone, and Suyangsan Southern Zone in North Korea.
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Soybean [
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AT-hook proteins are known to co-regulate transcription of genes through the modification of chromatin architecture. In plants, many genes encoding AT-hook proteins have been shown to be associated with increased seed yield or delayed senescence. In this study, we produced transgenic soybean plants overexpressing chromatin architecture-controlling
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The present study was conducted to evaluate the biological responses of soybean irradiated by gamma-rays. Four elite Korean soybean cultivars, Kwangankong, Shinpaldal-2, Shinhwakong, and Ilmikong, were irradiated with 100–400 Gy of gamma-rays. All cultivars showed significant reductions in morphological parameters. Seedling emergence rates of Kwangankong, Shinpaldal-2, Shinhwakong, and Ilmikong decreased by 46%, 31%, 44%, and 43% at 400 Gy, respectively. Plant height and fresh weight decreased with increasing dose. The median reduction dose (RD50) for plant height ranged from 184 to 278 Gy with an average of 212 Gy. The optimal dose of gamma irradiation for inducing mutation in the four elite soybean cultivars was in the range 200–300 Gy. Contents of malonaldehyde (MDA) in the gamma-ray irradiated plants were higher than those in the controls. With the exception of Kwangankong, activities of ascorbate peroxidase (APX) in other cultivars decreased at 100 Gy and started to increase significantly at 200 Gy and Shinhwakong cultivar had highest APX value observed at 300 Gy. No significant changes in peroxidase (POD) activity were observed in the Kwangankong, Shinpaldal-2, and Shinhwakong, while, POD activity increased with increasing dose in Ilmikong. In addition, gamma-ray treatments elicited a marked reduction in chlorophyll
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The soybean [
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Heat stress is one of the factors disturb productivity and growth of plants. Many genes including heat shock protein (HSP), heat shock transcription factors (HSF) and chaperones, were identified and characterized in many plants to play role in increased tolerance to abiotic stress. To reveal responsive gene to heat stress, we performed RNA-seq using two Korean soybean varieties under heat stress and normal conditions. The transcripts were analyzed, and we obtained 2,458 genes including 46 co-up regulation and 55 co-down regulated genes in both soybean varieties. We also revealed HSPs, HSFs and chaperones in the differentially expressed genes using BLAST and Pfam analyzation and verified expression changes under heat stress. Finally, we find 68 genes involved in HSP, HSF, chaperones in heat responsive genes associated increasing heat tolerance. As a result, relatively small HSP families were up regulated and continuously expressed in long period heat stress. On the other hand, large molecule HSPs, HSFs and chaperonin did not response to long heat stress. The expression profiling and characterization provide invaluable information to understand heat tolerance of soybean.
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Seed starch content (SSC) is a decisive factor influencing soy food quality. Variation in SSC affects the composition of major components, oil, and protein in soybean seeds. Therefore, understanding G × E interaction of SSC is important to produce soybeans with stable SSC. In the present study, G × E interactions of 17 soybean genotypes having different SSC (0.24–1.48%) and correlation of SSC with crude protein (CP) and crude fat (CF) were investigated. The genotypes were evaluated for SSC and other traits at two planting dates across three locations over two years (2015 and 2016). The genotype × year, genotype × location, and genotype × year × location interactions were found to be significant (
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AT-hook proteins of plant have shown to be involved in growth and development through the modification of chromatin architecture to co-regulate transcription of genes. Recently, many genes encoding AT-hook protein have been identified and their involvement in senescence delay is investigated. In this study, soybean transgenic plants overexpressing chromatin architecture-controlling
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The present study evaluated the biochemical effects of proton beam irradiation in soybean. Seeds of two Korean elite cultivars (Kwangan and Pungsannamul) were irradiated by a 57-MeV proton beam in the range of 50–400 Gy. We measured the contents of malondialdehyde (MDA), antioxidant enzymes, and chlorophyll. MDA contents in proton beam-irradiated plants were higher than those in control plants. The activity of antioxidant enzymes differed between the two cultivars. In Kwangan, ascorbate peroxidase (APX) activity increased by 29% relative to the control at 55 Gy and decreased by 58% at 308 Gy. Superoxide dismutase (SOD) and peroxidase (POD) activities decreased by 47 and 25% relative to the control at 55 Gy and recovered to 87 and 56% of that at 55 Gy with 172 Gy and 117 Gy, respectively. In Pungsannamul, APX and SOD decreased by 32 and 35% relative to the control at 62 Gy, with the highest value observed at 243 Gy. In terms of the chlorophyll content, the two varieties responded similarly to proton beam irradiation, whereas in Kwangan, no significant reduction was observed above 100 Gy when compared with the control. Proton beam irradiation affected chlorophyll b more than chlorophyll a. These results show that the activity of antioxidant enzymes decreased in response to irradiation with approximately 50 Gy proton beams, then increased gradually with increasing doses, followed by a moderate decrease at higher doses. According to correlation with MDA contents and antioxidant enzyme activities, defense system of Pungsannamul was less activated by proton-beam irradiation than that of Kwangan.
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Kazakhstan is an agrarian country with 270 million hectares utilized for animal and crop production. The foremost
objective
of the state agriculture programs in Kazakhstan is to achieve food security of country on the basis of competitive crop production. Demand for soybean as an oil crop and animal feed is steadily growing in Kazakhstan and hence soybeans can be a great attribute for food security in this region. Currently, over 90% of all soybean production is concentrated in one region (Almaty) because the crop is highly sensitive to photoperiod and temperature. The climatological conditions in majority of the region pose difficulties in growing the soybeans. In this review, we discussed the impact of the geographical and environmental conditions in enhancing the soybean cultivation in different parts of Kazakhstan. Additionally, we have taken an account of current status of soybean production and the barriers that may have great influence on the soybean yield. Because soybean is a short-day plant, the main role in its adaptation to areas in Kazakhstan is played by its
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First pod height (FPH) is an agronomic trait for the mechanical harvesting of soybeans with combines. The seed loss could be minimized, if the FPH is higher than the height of the cutter bar in combines. Hence, developing soybeans with high FPH has become one of important breeding goals in current crop improvement programs. The
objective
of this study was to evaluate genetic and environmental variation of FPH in soybean and to analyze the effect of ratio of FPH to plant height (PH) on seed yield. Four genotypes were evaluated across six different environments to analyze environmental variation of agronomic traits including FPH. Three F2 populations were evaluated to analyze genetic variation and relationship between the ratio of FPH to PH and seed yield. The main effects of planting distance, genotype and seeding date were significant for FPH, but FPH is affected more by genetic factors than by environmental factors. The mean heritability value of FPH was 66% across three F2 populations. Seed yield was found to reduce with increase in the FPH/PH ratio. In conclusion, genetic factors have effect more than environments to the variation of FPH. While FPH is higher than cutting height, the smaller ratio can minimize seed yield decrease.
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Plants have adapted to environmental challenges by expressing many plant genes in response to the stresses. Among those genes, CCCH zinc finger proteins are involved in abiotic and biotic stresses. Transgenic soybean plants overexpressing
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Soybean is an economically important leguminous seed crop for feed and food products that are rich in seed protein, oil and saccharides. Also, black soybean has been known as medicinal food. However, Kunitz trypsin inhibitor (KTI) and lectin proteins are a main antinutritional factor in mature black soybean seed. The genetic removal of the KTI and lectin proteins will improve the nutritional value of black soybean seed. The
objective
of this research was to develop a new black soybean lines with double recessive allele for both KTI and lectin protein. A total of 179 F2 seeds were obtained from the cross of ‘Gaechuck#1’ (
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Beta-carotene, a defense chemical, is synthesized by the carotenoid biosynthesis pathway. In the present study, a transgenic soybean line, with a single copy insertion of
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Soybean cyst [SCN,
objective
of this research was to evaluate these germplasm accessions for resistance to RKN and RN. The evaluation for RKN resistance was conducted in RKN infested field plantings after potatoes near Charleston, MO in 2006 and 2007. The evaluation for RN resistance was performed in a greenhouse at Fayetteville, AR, in 2007. Out of these accessions, 64 PIs were identified with high or moderate resistance to RKN. Of these 64 lines, 24 accessions showed good resistance to both RKN and RN. These new sources of resistance to multiple nematodes will be valuable materials for soybean breeding programs to develop new resistant cultivars that can overcome yield losses caused by one or more of these nematode species.
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Bacterial leaf pustule (BLP) caused by
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Soybean [
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Soybeans have a long history as a nutritious hay and silage crop. Early research extensively investigated forage yield, adaptability to various maturity zones, and nutritional values. Evaluation and breeding with diverse soybean accessions continued to optimize soybean forage yield and quality. There is still interest by breeders in developing more desirable forage soybeans, depending on market demand, and the existing interests of crop and livestock producers. In this review, we provide an update compiled from recent publications on the use and development of soybean as a forage crop.
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